Optical discrimination of type I and type III collagen through second order susceptibility imaging

Hsueh, Chiu-Mei; Tseng, Te-Yu; Lin, Hung‐Ming; Lee, Sheng-Lin; Huang, Ya-Der; Dong, Chongying

doi:10.1016/j.ijleo.2018.05.039

Cited by 2 publications

(5 citation statements)

References 11 publications

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“…Moreover, in other areas, it was found that the time‐lapse variations of collagen II production were not uniform. This result is similar to the previous study demonstrating the distinct differentiation rate of cells in two‐dimensional cultures .…”

Section: Resultssupporting

confidence: 92%

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Dynamic observation and quantification of type I/II collagen in chondrogenesis of mesenchymal stem cells by second‐order susceptibility microscopy

Hsueh

Lin

Tseng

et al. 2018

Journal of Biophotonics

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Second-order susceptibility (SOS) microscopy is used to image and characterize chondrogenesis in cultured human mesenchymal stem cells. SOS analysis shows that the SOS tensor ratios can be used to characterize type I and II collagens in living tissues and that both collagen types are produced at the onset of chondrogenesis. Time-lapse analysis shows a modulation of extracellular matrix results in a higher rate in increase of type II collagen, as compared to type I collagen. With time, type II collagen content stabilizes at the composition of 70% of total collagen content. SOS microscopy can be used to continuously and noninvasively monitor the production of collagens I and II. With additional development, this technique can be developed into an effective quality control tool for monitoring extracellular matrix production in engineered tissues.

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Section: Resultssupporting

confidence: 92%

“…Specifically, χ zzz / χ zxx are 1.4 for type I collagen and 1.2 for type II collagen. Moreover, χ xzx / χ zxx are 0.7 and 0.4 for type I and II collagens, respectively . Representative profiles of SOS tensor rations for type I collagen (rat tail tendon) and II collagen (rat trachea) are shown in Figure .…”

Section: Methodsmentioning

confidence: 99%

Dynamic observation and quantification of type I/II collagen in chondrogenesis of mesenchymal stem cells by second‐order susceptibility microscopy

Hsueh

Lin

Tseng

et al. 2018

Journal of Biophotonics

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“…[91]. Recently, Dong et al has demonstrated the optical discrimination of type I and type III in rat skin while Rajesh et al has shown the detection capability of type I and type II collagen in osteoarthritic human cartilage [90,140]. The ovary is a highly dynamic tissue whose ECM composition is changing continuously.…”

Section: Susceptibility Based Shg Microscopymentioning

confidence: 99%

“…In general, susceptibility based SHG microscopy can quantitatively discriminate several harmonophores (e.g., collagen type I, II, III, muscles etc.) and thus could emerge as a powerful tool for detecting early-stage ECM modification at molecular level with high sensitivity, and can contribute in understanding various associated pathophysiological process [130,133,139,140].…”

Section: Susceptibility Based Shg Microscopymentioning

confidence: 99%

“…SHG images of tissue are acquired by the rotation of linearly polarized light a. A new label-free contrast mechanism to detect the morphological and structural changes and 3D molecular orientation b. Differentiate the type of SHG molecular sources (selective imaging) e.g., collagen type I and collagen type-II and, can provide their relative quantification c. Allow quantitative detection of a change in anisotropy d. Allow determination of methylene and peptide pitch angle in collagen that is beyond the limit of optical resolution Same as SHG [90,135,140] Stokes vector based SHG Sort SHG into four different polarization components and represent them as a Stokes vector a. A contrast mechanism to characterize the polarization properties of SHG, molecular structure, and orientation b.…”

Section: Shg-circular Dichroism (Cd)mentioning

confidence: 99%

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Label-Free Non-linear Multimodal Optical Microscopy—Basics, Development, and Applications

et al. 2019

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Non-linear optical (NLO) microscopy has proven to be a powerful tool especially for tissue imaging with sub-cellular resolution, high penetration depth, endogenous contrast specificity, pinhole-less optical sectioning capability. In this review, we discuss label-free non-linear optical microscopes including the two-photon fluorescence (TPF), fluorescence lifetime imaging microscopy (FLIM), polarization-resolved second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) techniques with various samples. The non-linear signals are generated from collagen in tissue (SHG), amylopectin from starch granules (SHG), sarcomere structure of fresh muscle (SHG), elastin in skin (TPF), nicotinamide adenine dinucleotide (NADH) in cells (TPF), and lipid droplets in cells (CARS). Again, the non-linear signals are very specific to the molecular structure of the sample and its relative orientation to the polarization of the incident light. Thus, polarization-resolved non-linear optical microscopy provides high image contrast and quantitative estimate of sample orientation. An overview of the advancements on polarization-resolved SHG microscopy including Stokes vector based polarimetry, circular dichroism, and susceptibility are also presented in this review article. The working principles and corresponding implements of above-mentioned microscopy techniques are elucidated. The potential of time-resolved TPF lifetime imaging microscopy (TP-FLIM) is explored by imaging endogenous fluorescence of NAD(P)H, a key coenzyme in cellular metabolic processes. We also discuss single laser source time-resolved multimodal CARS-FLIM microscopy using time-correlated single-photon counting (TCSPC) in combination with continuum generation from photonic crystal fiber (PCF). Using examples, we demonstrate that the multimodal NLO microscopy is a powerful tool to assess the molecular specificity with high resolution.

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Optical discrimination of type I and type III collagen through second order susceptibility imaging

Cited by 2 publications

References 11 publications

Dynamic observation and quantification of type I/II collagen in chondrogenesis of mesenchymal stem cells by second‐order susceptibility microscopy

Dynamic observation and quantification of type I/II collagen in chondrogenesis of mesenchymal stem cells by second‐order susceptibility microscopy

Label-Free Non-linear Multimodal Optical Microscopy—Basics, Development, and Applications

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