Optical control of calcium-regulated exocytosis

Izquierdo-Serra, Mercè; Trauner, Dirk; Llobet, Artur; Gorostiza, Pau

doi:10.1016/j.bbagen.2012.11.003

Cited by 15 publications

(19 citation statements)

References 41 publications

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“…However, the large, step-function photocurrents provided by MAG and the calcium permeability of GluK2 40 make LiGluR-MAG more attractive to trigger calcium-regulated processes 39,41 including astrocyte activation 38 (see also Figure S7 in the Supporting Information). In cultured astrocytes expressing LiGluR-MAG (Figures 8), two-photon excitation at 820 nm triggered bistable currents (Figure 8c).…”

Section: Resultsmentioning

confidence: 99%

Two-Photon Neuronal and Astrocytic Stimulation with Azobenzene-Based Photoswitches

et al. 2014

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Synthetic photochromic compounds can be designed to control a variety of proteins and their biochemical functions in living cells, but the high spatiotemporal precision and tissue penetration of two-photon stimulation have never been investigated in these molecules. Here we demonstrate two-photon excitation of azobenzene-based protein switches and versatile strategies to enhance their photochemical responses. This enables new applications to control the activation of neurons and astrocytes with cellular and subcellular resolution.

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Section: Resultsmentioning

confidence: 99%

Two-Photon Neuronal and Astrocytic Stimulation with Azobenzene-Based Photoswitches

et al. 2014

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“…11 Even in cases where labeling, photoswitching, or the ligand efficacy remain submaximal, LiGluR constitutes a powerful tool for in vivo studies, as low-affinity kainate receptors like GluK2 are not fully activated in many physiological conditions either (see SI). LiGluR has been used to evoke patterns of action potentials in neurons, 12 reproducibly inject calcium into glial cells and chromaffin cells to evoke transmitter release, 13,14 excite specific cells for neural circuit analysis in vivo , 15 and restore a retinal light response and visual behavior to mice blinded by photoreceptor cell degeneration. 16 …”

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confidence: 99%

A Red-Shifted, Fast-Relaxing Azobenzene Photoswitch for Visible Light Control of an Ionotropic Glutamate Receptor

et al. 2013

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The use of azobenzene photoswitches has become a dependable method for rapid and exact modulation of biological processes and material science systems. The requirement of ultraviolet light for azobenzene isomerization is not ideal for biological systems due to poor tissue penetration and potentially damaging effects. While modified azobenzene cores with a red-shifted cis-to-trans isomerization have been previously described, they have not yet been incorporated into a powerful method to control protein function: the photoswitchable tethered ligand (PTL) approach. We report the synthesis and characterization of a red-shifted PTL, L-MAG0460, for the light-gated ionotropic glutamate receptor LiGluR. In cultured mammalian cells, the LiGluR +L-MAG0460 system is activated rapidly by illumination with 400–520 nm light to generate a large ionic current. The current rapidly turns off in the dark as the PTL relaxes thermally back to the trans configuration. The visible light excitation and single-wavelength behavior considerably simplify use and should improve utilization in tissue.

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“…Another example is the exclusive activation of postsynaptic receptors, which allowed Kauwe et al to discover a novel plasticity mechanism that is induced post-synaptically and expressed pre-synaptically via a retrograde signal at the fly neuromuscular junction (Figure 4d) [37]. LiGluR has also been used to control Ca 2+ -influx into astrocytes to trigger gliotransmission (Figure 4c) [38], or to trigger exocytosis from neuroendocrine cells [39]. In addition, spatially-targeted 2-photon uncaging of glutamate has been applied at synapses in vivo in mice demonstrating the applicability of chemical based approaches in the mammalian brain (Figure 4e) [7].…”

Section: Optical Control Of Glutamate Receptors With Temporal Resolutmentioning

confidence: 99%

Controlling ionotropic and metabotropic glutamate receptors with light: principles and potential

Reiner

Levitz

Isacoff

2015

Current Opinion in Pharmacology

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Light offers unique advantages for studying and manipulating biomolecules and the cellular processes that they control. Optical control of ionotropic and metabotropic glutamate receptors has garnered significant interest, since these receptors are central to signaling at neuronal synapses and only optical approaches provide the spatial and temporal resolution required to directly probe receptor function in cells and tissue. Following the classical method of glutamate photo-uncaging, recently developed methods have added other forms of remote control, including those with high molecular specificity and genetic targeting. These tools open the door to the direct optical control of synaptic transmission and plasticity, as well as the probing of native receptor function in intact neural circuits.

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Optical control of calcium-regulated exocytosis

Cited by 15 publications

References 41 publications

Two-Photon Neuronal and Astrocytic Stimulation with Azobenzene-Based Photoswitches

Two-Photon Neuronal and Astrocytic Stimulation with Azobenzene-Based Photoswitches

A Red-Shifted, Fast-Relaxing Azobenzene Photoswitch for Visible Light Control of an Ionotropic Glutamate Receptor

Controlling ionotropic and metabotropic glutamate receptors with light: principles and potential

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