Optical absorbance-based rapid test for the detection of sickle cell trait and sickle cell disease at the point-of-care

Srinivasan, Rajesh; Christo, V R Eugene; Kunnath, Radhika Nambannor; Katare, Prateek; Venukumar, Aravind; Nambison, Nisanth K.M.; Gorthi, Sai Siva

doi:10.1016/j.saa.2022.121394

Cited by 3 publications

(3 citation statements)

References 24 publications

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“…Alkaline electrophoresis is an analytical tool that has been used to distinguish thalassemia and sickle cell anemia at pH 8.4. First, a hemolysate is prepared from the red blood cells; then, it is added to a cellulose strip and run-in buffer at a constant voltage in an electrophoresis chamber (33).…”

Section: Peripheral Blood Smearmentioning

confidence: 99%

Evaluation of Current Clinical Research Spectrum of Sickle Cell Anemia to Approach New Innovative Developmental Techniques for Enhancing Sudanese Health Systems

Salih

Saad

Eldouch³

et al. 2022

Preprint

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Background Sickle cell Anemia and/or Disease (SCA/SCD) is an autosomal recessive disease leading to abnormal hemoglobin with global prevalence in general and tribal burden in sub-Saharan Africa particularly. Methods Recent patients’ assessments were done using routine diagnostic protocols for SCA monitoring and manifestations. Insight of the physicians towards innovative techniques at the molecular level were done to enhance medical performance of the disease investigations. The online questionnaire showed the response and acceptance levels of the physicians to introduced innovative techniques in integration with current clinical and laboratory’s spectrums. Data interpretation and statistical analysis were done using IBM-SPSS ver 25 and MS. Excel 2019. Results Clinical manifestation showed painful crises, (Hand and food syndrome) and enuresis were observed in more than half of the patients, while stroke observed only in 5.5%. A strong association was observed in males regarding Hb vs RBC and MCV vs HbA2.However. females show an association between MCV vs HbA2 only. SCA current spectrum include CBC, BF and Solubility Sickling Test showed significant results in Hemoglobin P < 0.05 and Strong association was observed between Hb vs RBC, MCV vs HbA2 for males and MCV vs HbA2 for female only. Polymerase Chain Reaction (PCR) was introduced to detect inherited polymorphic traits in infants and parents beside the flow cytometry gave a detailed informatic image for the up normal blood cells shape and amounts comparing with normal ones. The high response of the online questionnaire (100%) showed the attitude of doctors and related staff accepting innovative techniques for enhancing current health system. Conclusions Although the current clinical and laboratory diagnostic protocols are effective for diagnosing SCA, the follow-up and treatment but the approach of innovative techniques is highly demanded for detecting un-seen symptoms and hidden polymorphic traits, beside monitoring all patients with including other family members.

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Section: Peripheral Blood Smearmentioning

confidence: 99%

Evaluation of Current Clinical Research Spectrum of Sickle Cell Anemia to Approach New Innovative Developmental Techniques for Enhancing Sudanese Health Systems

Salih

Saad

Eldouch³

et al. 2022

Preprint

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“…It shows high diagnostic accuracy when the electrophoresis chip is imaged with the help of a Gazelle reader 18 . SickleCERT is an absorption‐based test and needs a customized spectrophotometer 19 . Table S1 shows a comparison between the existing POC tests.…”

Section: Introductionmentioning

confidence: 99%

“… 18 SickleCERT is an absorption‐based test and needs a customized spectrophotometer. 19 Table S1 shows a comparison between the existing POC tests. Due to the magnitude of the population that needs screening in affected LMICs, 4 , 5 we need to supplement existing tests with other POC technologies.…”

Section: Introductionmentioning

confidence: 99%

Differential sensitivity to hypoxia enables shape‐based classification of sickle cell disease and trait blood samples at point of care

D'Costa,

Sharma,

Manna

et al. 2023

Bioengineering & Transla Med

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Red blood cells (RBCs) become sickle‐shaped and stiff under hypoxia as a consequence of hemoglobin (Hb) polymerization in sickle cell anemia. Distinguishing between sickle cell disease and trait is crucial during the diagnosis of sickle cell disease. While genetic analysis or high‐performance liquid chromatography (HPLC) can accurately differentiate between these two genotypes, these tests are unsuitable for field use. Here, we report a novel microscopy‐based diagnostic test called ShapeDx™ to distinguish between disease and trait blood in less than 1 h. This is achieved by mixing an unknown blood sample with low and high concentrations of a chemical oxygen scavenger and thereby subjecting the blood to slow and fast hypoxia, respectively. The different rates of Hb polymerization resulting from slow and fast hypoxia lead to two distinct RBC shape distributions in the same blood sample, which allows us to identify it as healthy, trait, or disease. The controlled hypoxic environment necessary for differential Hb polymerization is generated using an imaging microchamber, which also reduces the sickling time of trait blood from several hours to just 30 min. In a single‐blinded proof‐of‐concept study conducted on a small cohort of clinical samples, the results of the ShapeDx™ test were 100% concordant with HPLC results. Additionally, our field studies have demonstrated that ShapeDx™ is the first reported microscopy test capable of distinguishing between sickle cell disease and trait samples in resource‐limited settings with the same accuracy as a gold standard test.

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Microfluidic Point-of-Care Diagnostics for Multi-Disease Detection Using Optical Techniques: A Review

Ahmadsaidulu

Banik

Kumar³

et al. 2024

IEEE Trans.on Nanobioscience

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Optical absorbance-based rapid test for the detection of sickle cell trait and sickle cell disease at the point-of-care

Cited by 3 publications

References 24 publications

Evaluation of Current Clinical Research Spectrum of Sickle Cell Anemia to Approach New Innovative Developmental Techniques for Enhancing Sudanese Health Systems

Evaluation of Current Clinical Research Spectrum of Sickle Cell Anemia to Approach New Innovative Developmental Techniques for Enhancing Sudanese Health Systems

Differential sensitivity to hypoxia enables shape‐based classification of sickle cell disease and trait blood samples at point of care

Microfluidic Point-of-Care Diagnostics for Multi-Disease Detection Using Optical Techniques: A Review

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