Optical 4D oxygen mapping of microperfused tissue models with tunable in vivo-like 3D oxygen microenvironments

Abstract

Sufficient and controllable oxygen supply is essential for in vitro 3D cell and tissue culture at high cell densities, which calls for volumetric in situ oxygen analysis methods to quantitatively...

Oxygen-releasing biomaterials for regenerative medicine

“…104 Hopefully, monitoring the oxygen distribution can help clarify this issue and provide a reliable reference for oxygen supply to engineered tissues. Recently, Larsen and his co-workers attempted to build dynamic microperfused tissue models with optical 4D oxygen mapping capability by combining a microfluidic device and an embedded hydrogel complex that is dispersed in commercially available oxygen-sensing microbeads CPOx-50-PtP and Hep G2 human hepatocyte carcinoma cells 99 (Fig. 4a).…”

Fluorescent nano- and microparticles for sensing cellular microenvironment: past, present and future applications

“…8c ). 240 Finally, hybrid systems, including pH-fluorescent sensors, were developed by combining different technologies to probe 3D cell growth and tissue regeneration. 241 Capsules based on SNARF-1 were included in a 3D additive-manufactured scaffold with controlled geometry and porosity by providing a real-time detection of the acidification of human mesenchymal stromal cells.…”

Physiological oxygen measurements in vitro-Schrödinger’s cat in 3D cell biology

“…A similar system, capable of increasing the throughput by automation and therefore, to perform microphysiological measurements in real-time over extended cultivation periods, was introduced by Eggert et al (2021) . A phosphorescence lifetime-based system is able to measure the dissolved oxygen concentration even within 3D cellular constructs by incorporating oxygen-sensitive microbeads ( Weyand et al, 2015 ; Wesseler et al, 2022 ). However, as discussed above, these measurement techniques only deliver spot-like measurement data, may destroy the tissue architecture within the piercing channel with the associated adverse effects on the cells, and are not able to characterize the cells’ metabolism further.…”