Operation Everest III (Comex'97): the effect of simulated severe hypobaric hypoxia on lipid peroxidation and antioxidant defence systems in human blood at rest and after maximal exercise

Joanny, P; Steinberg, Johannes; Robach, Paul; Richalet, Jean‐Paul; Gortan, Claude; Gardette, B; Jammes, Yves

doi:10.1016/s0300-9572(00)00373-7

Cited by 114 publications

(84 citation statements)

References 32 publications

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“…In contrast, the maximal exercise performed under hypoxia induced a significant increase in plasma MDA ( þ 56%) and AOPP ( þ 44%). Cellular generation of ROS under hypoxia was reported previously in animals at rest (Hitka et al, 2003), and in humans at high altitude (Simon-Schnass, 1996;Jefferson et al, 2004) and in hypobaric chambers (Joanny et al, 2001).…”

Section: Effects Of Maximal Exercise Under Normobaric Hypoxia and Normentioning

confidence: 55%

“…In resting conditions, oxidative stress markers have been detected in humans under hypobaric hypoxia (Bailey et al, 2001a;Jefferson et al, 2004), at simulated altitude in hypobaric chambers (Joanny et al, 2001) and after breathing gas mixture reduced in O 2 content (Wing et al, 2003). Firstly, the increase in catecholamine production under hypoxic conditions (Mazzeo et al, 1998) could be responsible for oxidative stress.…”

Section: Kàmentioning

confidence: 99%

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Effects of exercise and training in hypoxia on antioxidant/pro-oxidant balance

Pialoux¹,

Mounier²,

Ponsot

et al. 2006

Eur J Clin Nutr

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Objective: The aim was to investigate the effects of acute exercise under hypoxic condition and the repetition of such exercise in a 'living low-training high' training on the antioxidant/prooxidant balance. Design: Randomized, repeated measures design. Setting: Faculté de Médecine, Clermont-Ferrand, France. Subjects: Fourteen runners were randomly divided into two groups. A 6-week endurance training protocol integrated two running sessions per week at the second ventilatory threshold into the usual training. Intervention: A 6-week endurance training protocol integrated two running sessions per week at the second ventilatory threshold into the usual training. The first hypoxic group (HG, n ¼ 8) carried out these sessions under hypoxia (3000 m simulated altitude) and the second normoxic group (NG, n ¼ 6) in normoxia. In control period, the runners were submitted to two incremental cycling tests performed in normoxia and under hypoxia (simulated altitude of 3000 m). Plasma levels of advanced oxidation protein products (AOPP), malondialdehydes (MDA) and lipid oxidizability, ferric-reducing antioxidant power (FRAP), lipid-soluble antioxidants (a-tocopherol and b-carotene) normalized for triacyglycerols and cholesterol were measured before and after the two incremental tests and at rest before and after training. Results: No significant changes of MDA and AOPP level were observed after normoxic exercise, whereas hypoxic exercise induced a 56% rise of MDA and a 44% rise of AOPP. Plasma level of MDA and arterial oxygen hemoglobin desaturations after the acute both exercises were highly correlated (r ¼ 0.73). a-Tocopherol normalized for cholesterol and triacyglycerols increased only after hypoxic exercise (10-12%, Po0.01). After training, FRAP resting values (À21%, Po0.05) and a-tocopherol/ triacyglycerols ratio (À24%, Po0.05) were diminished for HG, whereas NG values remained unchanged. Conclusions: Intense exercise and hypoxia exposure may have a cumulative effect on oxidative stress. As a consequence, the repetition of such exercise characterizing the 'living low-training high' model has weakened the antioxidant capacities of the athletes. Sponsorship: International Olympic Committee and the Direction Régionale de la Jeunesse et des Sports de la Région Auvergne.

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Section: Effects Of Maximal Exercise Under Normobaric Hypoxia and Normentioning

confidence: 55%

Section: Kàmentioning

confidence: 99%

Effects of exercise and training in hypoxia on antioxidant/pro-oxidant balance

Pialoux¹,

Mounier²,

Ponsot

et al. 2006

Eur J Clin Nutr

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“…It is known that acute exercise in hypoxia (Araneda et al 2005;Bailey et al 2001;Joanny et al 2001;Moller et al 2001;Pialoux et al 2006) and normoxia (Bailey et al 2001;Bailey et al 2004) increases oxidative stress compared to resting conditions. On the other hand, regular intermittent hypoxic training was leading to attenuated increases in lipid peroxidation and reduced plasma malondialdehyde due to mobilization of α-tocopherol compared with normoxic training (Bailey et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Moderate altitude but not additional endurance training increases markers of oxidative stress in exhaled breath condensate

et al. 2009

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Oxidative stress occurs at altitude, and physical exertion might enhance this stress. In the present study, we investigated the combined effects of exercise and moderate altitude on redox balance in ten endurance exercising biathletes, and five sedentary volunteers during a 6 week stay at 2800 m. As a marker for oxidative stress, hydrogen peroxide (H 2 O 2 ) was analyzed by the biosensor measuring system Ecocheck TM , and 8-iso prostaglandin F2α (8-iso PGF2α) was determined by enzyme immunoassay in exhaled breath condensate (EBC). To determine the whole blood antioxidative capacity, we measured reduced glutathione (GSH) enzymatically using Ellman's reagent. Exercising athletes and sedentary volunteers showed increased levels of oxidative markers at moderate altitude, contrary to our expectations, there was no difference between both groups. Therefore, all subjects' data were pooled to examine the oxidative stress response exclusively due to altitude exposure. H 2 O 2 levels increased at altitude and remained elevated for 3 days after returning to sea level (p ≤ 0.05). On the other hand, 8-iso PGF2α levels showed a tendency to increase at altitude, but declined immediately after returning to sea level (p ≤ 0.001).Hypoxic exposure during the first day at altitude resulted in elevated GSH levels (p ≤ 0.05), that decreased during prolonged sojourn at altitude (p ≤ 0.001). In conclusion, a stay at moderate altitude for up to 6 weeks increases markers of oxidative stress in EBC independent of additional endurance training. Notably, this oxidative stress is still detectable 3 days upon return to sea level.

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“…On other hand, periods of hypoxia could also cause an oxidative stress (Joanny et al, 2001;Bailey et al, 2001a;Wing et al, 2003). We previously demonstrated (unpublished data) that a 3-h exposure to hypoxia, at rest, at a simulated altitude of 3000 m induced an increase in oxidized lipids (malondialdehydes, MDA) and in advanced oxidation proteins products (AOPP) in elite endurance athletes known to have strong enzymatic antioxidant defenses (Robertson et al, 1991;Urso and Clarkson, 2003).…”

Section: Introductionmentioning

confidence: 96%

Effects of the ‘live high–train low’ method on prooxidant/antioxidant balance on elite athletes

Pialoux¹,

Mounier²,

Rock

et al. 2008

Eur J Clin Nutr

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Background/Objectives: We previously demonstrated that acute exposure to hypoxia (3 h at 3000 m) increased oxidative stress markers. Thus, by using the 'living high-training low' (LHTL) method, we further hypothesized that intermittent hypoxia associated with endurance training alters the prooxidant/antioxidant balance. Subjects/Methods: Twelve elite athletes from the Athletic French Federation were subjected to 18-day endurance training. They were divided into two groups: one group (control group) trained at 1200 m and lived in hypoxia (2500-3000 m simulated altitude) and the second group trained and lived at 1200 m. The subjects performed an acute hypoxic test (10 min at 4800 m) before and immediately after the training. Plasma levels of advanced oxidation protein products (AOPP), malondialdehydes (MDA), ferric-reducing antioxidant power (FRAP), lipid-soluble antioxidants normalized for triacylglycerols, and cholesterol and retinol were measured before and after the 4800 m tests. Results: After the training, MDA and AOPP concentrations were decreased in response to the 4800 m test only for the control group. Eighteen days of LHTL induced a significant decrease of all antioxidant markers (FRAP, P ¼ 0.01; a-tocopherol, P ¼ 0.04; b-carotene, P ¼ 0.01 and lycopene, P ¼ 0.02) for the runners. This imbalance between antioxidant and prooxidant might result from insufficient intakes in vitamins A and E. Conclusions: The LHTL model characterized by the association of aerobic exercises and intermittent resting hypoxia exposures decreased the antioxidant status whereas the normoxic endurance training induced preconditioning mechanisms in response to the 4800 m test.

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Operation Everest III (Comex'97): the effect of simulated severe hypobaric hypoxia on lipid peroxidation and antioxidant defence systems in human blood at rest and after maximal exercise

Cited by 114 publications

References 32 publications

Effects of exercise and training in hypoxia on antioxidant/pro-oxidant balance

Effects of exercise and training in hypoxia on antioxidant/pro-oxidant balance

Moderate altitude but not additional endurance training increases markers of oxidative stress in exhaled breath condensate

Effects of the ‘live high–train low’ method on prooxidant/antioxidant balance on elite athletes

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