Oocyte development and ovarian maturation of the black triggerfish, Melichthys niger(Actinopterygii: Balistidae) in São Pedro e São Paulo Archipelago, Brazil

Abstract

The oogenesis is a key stage in the reproductive development of an organism, which can be best understood from histological analysis of ovaries in different maturity stages. In order to provide information on the reproductive biology of the black triggerfish, M. niger, in particular on its oogenesis process, this study aimed at identifying and characterizing the oocyte development stages and its organization within the different stages of ovarian maturation based on specimens from São Pedro e São Paulo Archipelago. In this present report, a number of 294 ovaries were histologically analyzed. It was verified that they are composed of ovigerous lamellae containing oocytes at different development stages. Five different stages of oogenesis were identified: young cells, with an average size of 12.9 ìm; previtellogenic oocytes (perinucleolar), with an average size of 53.5 ìm; cortical-alveoli oocytes with an average size of 83.1 ìm; vitellogenic oocytes, with an average size of 160.4 ìm and mature oocytes, with an average size of 289.8 ìm. In addition to the germ cells, some somatic structures were also identified, such as: ovarian wall, follicular cells and blood vessels. Based on the type and number of oocytes observed, four stages of ovarian maturation were identified: early maturation, represented by only 2.2% of the sample; middle maturation, represented by 9.9%; mature, represented by 44.2% and resting, represented by 43.9%. The identification of five oocyte development stages in the ovarians from M. niger, suggested that the specie follows a pattern similar to that described for other marine fish.A oogênese é um estágio chave no desenvolvimento reprodutivo de um organismo, o qual pode ser melhor compreendido a partir de análises histológicas dos ovários em diferentes estágios de maturidade. A fim de fornecer informações sobre a biologia reprodutiva do cangulo-preto, M. niger, em especial sobre o seu processo de oogênese, este estudo teve como objetivo identificar e caracterizar as fases do desenvolvimento ovocitário e sua organização dentro dos diferentes estágios de maturação ovariana, baseado em espécimes do Arquipélago de São Pedro e São Paulo. No presente estudo, um número de 294 ovários foram analisados histologicamente. Foi verificado que eles são constituídos por lamelas ovígeras contendo ovócitos em diferentes estágios de desenvolvimento. Foram identificados cinco diferentes estágios da oogênese: células jovens, com tamanho médio de 12,9 ìm; ovócitos pré-vitelogênicos (perinucleolares), com tamanho médio de 53,5 ìm; ovócitos alvéolo-corticais, com tamanho médio de 83,1 ìm; ovócitos vitelogênicos, com tamanho médio de 160,4 ìm e ovócitos maduros, com tamanho médio de 289,8 ìm. Além das células germinativas, algumas estruturas somáticas também foram identificadas, tais como: parede do ovário, células foliculares e vasos sanguíneos. Baseado no tipo e número de ovócitos observados, quatro estágios de maturação ovariana foram identificados: início de maturação, representado por apenas 2,2% da amostra; média...

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“…The cortical alveoli stage was observed in the ovaries of P. stridens at the end of the previtellogenic stage; nevertheless, this structure in other species may appear only in the late previtellogenic stage (Cardenas, Chávez, González, Espinosa, & Jiménez-garcía, 2008) or only in later stages of the oocyte development (Tricas and Hiramoto, 1989). The vitellogenic stage of P. stridens, as in the majority of teleosts, was marked by a significant increase in the oocyte sizes, mainly by the inclusion of lipid droplets in cell cytoplasm, a process that begins in the previous stage (II, III and IV) by the exogenous accumulation of proteins ( Branco et al, 2013). The final oocyte development stage was identified in the present work and was characterized by mature oocytes.…”

“…www.trjfas.org ISSN 1303-2712DOI: 10.4194/1303-2712 was also reported by Robillard, Reiss, and Jones (2008) and Branco et al (2013). In the majority of teleosts, the mature oocyte development stage is featured by a rapid increase in follicular volume, depending on the hydration and accumulation of macromolecules (Murua and Saborido-Rey, 2003).…”

“…However, P. stridens has batch spawning during the spawning, and the ovaries have an asynchrony pattern. It appears that the increase in weight and ovarian volume is related to the vitellogenic stages in the first oocyte development (Branco, Viana, Félix, Véras, & Hazin, 2013). The cortical alveoli stage was observed in the ovaries of P. stridens at the end of the previtellogenic stage; nevertheless, this structure in other species may appear only in the late previtellogenic stage (Cardenas, Chávez, González, Espinosa, & Jiménez-garcía, 2008) or only in later stages of the oocyte development (Tricas and Hiramoto, 1989).…”

Gonad development and reproductive cycle of the white-spotted rabbitfish Siganus canaliculatus (Park, 1797) in Palompon, Leyte, Eastern Visayas, Philippines

“…Field determination was based on the external characteristics of the gonads (Ntiba & Jaccarini, 1990). Staging of histological slides was done through LW Scientific (Lawrenceville, GA) compound light microscope based on the appearance of the most advanced cells (West, 1990), applying the criteria described by Yamamoto (1965;in Wassef & Hady, 2001) and other related information from the literature (Branco, Viana, Felix, Veras, & Hazin, 2013;Marzouqi, Jayabalan, Al-Nahdi, & Al-Anbory, 2011). This specific study covered the last year of a larger project conducted from 2009 to 2012.…”

Reproductive strategies of the marine fishes from the southwest Atlantic Ocean: an application of histological and image processing techniques

“…The inner ARAUJO; CHELLAPPA, 2002;ANDRADE et al, 2003;FAVARO et al, 2005;FAVARO et al, 2007;SILVA et al, 2005;BESSA et al, 2007;ARAÚJO et al, 2008;ALBIERI;ARAÚJO, 2010;FÁVARO, 2010;MARQUES;FERREIRA, 2011;FÁVARO, 2011;FERNANDES et al, 2012;CASSACA et al, 2012;BRANCO et al, 2013;BEZERRA et al, 2013;DIAS, 2013;CONDINI et al, 2014;LEMOS et al, 2014;CHELLAPPA, 2014). Thus, the application of histological methods are still scarce, and some of these authors have…”