Ontogeny of B cells expressing IgM in embryonic and larval tissues of the American grass frog,Rana pipiens

Zettergren, Leslie D.

doi:10.1002/(sici)1097-010x(20000601)286:7<736::aid-jez8>3.0.co;2-g

Cited by 3 publications

(1 citation statement)

References 45 publications

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“…Spadefoots are among the fastest developing anuran species due to their association with ephemeral breeding sites [51] , [52] . Zettergren [53] reported cells synthesizing immunoglobulins (Ig) during embryogenesis and B lymphocytes circulating in pre-metamorphic L. pipiens at the onset of feeding. Leukocyte mobilization and anti-FV3 IgY antibody production have been reported as immune responses to ranavirus infection in X. laevis [54] , [55] .…”

Section: Discussionmentioning

confidence: 99%

Development and Disease: How Susceptibility to an Emerging Pathogen Changes through Anuran Development

et al. 2011

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Ranaviruses have caused die-offs of amphibians across the globe. In North America, these pathogens cause more amphibian mortality events than any other pathogen. Field observations suggest that ranavirus epizootics in amphibian communities are common during metamorphosis, presumably due to changes in immune function. However, few controlled studies have compared the relative susceptibility of amphibians to ranaviruses across life stages. Our objectives were to measure differences in mortality and infection prevalence following exposure to ranavirus at four developmental stages and determine whether the differences were consistent among seven anuran species. Based on previous studies, we hypothesized that susceptibility to ranavirus would be greatest at metamorphosis. Our results did not support this hypothesis, as four of the species were most susceptible to ranavirus during the larval or hatchling stages. The embryo stage had the lowest susceptibility among species probably due to the protective membranous layers of the egg. Our results indicate that generalizations should be made cautiously about patterns of susceptibility to ranaviruses among amphibian developmental stages and species. Further, if early developmental stages of amphibians are susceptible to ranaviruses, the impact of ranavirus epizootic events may be greater than realized due to the greater difficulty of detecting morbid hatchlings and larvae compared to metamorphs.

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Section: Discussionmentioning

confidence: 99%

Development and Disease: How Susceptibility to an Emerging Pathogen Changes through Anuran Development

et al. 2011

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Do immune system changes at metamorphosis predict vulnerability to chytridiomycosis? An update

Humphries

Lanctôt

Robert

et al. 2022

Developmental & Comparative Immunology

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Role of the Mesonephros as a Transient Haematopoietic Organ in the Bovine Embryo

Kritzenberger¹,

Wrobel

2010

Anat Histol Embryol

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During vertebrate embryogenesis, haematopoietic stem cells (HSC) arise in the aorta-gonads-mesonephros (AGM) region. In the present study, we examined serial sections of 22-34 days post-insemination (dpi) bovine embryos, a species with a well-developed and functional mesonephros. We describe the temporo-spatial distribution of presumptive c-kit positive HSC and the occurrence of haematopoietic foci in the mesonephros and fetal liver using specific antibodies directed against haematopoietic cell markers and conventional electron microscopy. In the mesonephros, presumptive HSC were found at 23-24 dpi in the blood stream, in the endothelial lining of the filtering capillaries and in the septal stroma of the cranial part of the mesonephros, the mesonephric giant corpuscle (MGC), suggesting a colonalization via the blood stream from the haematopoietic clusters of the dorsal aorta. From 25 to 30 dpi, presumptive HSC predominate in the septal stroma of the MGC, were they first expand but then decline and disappear following 32 dpi. In parallel, we found ongoing erythropoiesis and myelopoiesis starting in the MGC at 24 dpi and extending during the complete observation period. In the embryonic liver, colonization with presumptive c-kit positive HSC occurs slightly later, at 25 dpi. Active formation of blood cells in the liver increases following 30 dpi. In conclusion, the mesonephros of bovine embryos, in particular its MGC, functions as a primitive haematopoietic organ, temporarily intercalated between extraembryonic erythropoiesis and haematopoiesis within in the fetal liver, thus recapitulating for a short period a phylogenetically old site of blood formation.

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Ontogeny of B cells expressing IgM in embryonic and larval tissues of the American grass frog,Rana pipiens

Cited by 3 publications

References 45 publications

Development and Disease: How Susceptibility to an Emerging Pathogen Changes through Anuran Development

Development and Disease: How Susceptibility to an Emerging Pathogen Changes through Anuran Development

Do immune system changes at metamorphosis predict vulnerability to chytridiomycosis? An update

Role of the Mesonephros as a Transient Haematopoietic Organ in the Bovine Embryo

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