One-step synthesis of gradient gadolinium ironhexacyanoferrate nanoparticles: a new particle design easily combining MRI contrast and photothermal therapy

Li, Yichen; Li, Carissa H.; Talham, Daniel R.

doi:10.1039/c4nr06481j

Cited by 41 publications

(32 citation statements)

References 41 publications

(68 reference statements)

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“…13 nm AuNPs were fabricated through the reduction of HAuCl 4 with citrates 32 . In brief, HAuCl 4 •3H 2 O (39.4 mg) was added into a round-bottom flask containing 100 mL deionized water.…”

Section: Methodsmentioning

confidence: 99%

Nanozyme-assisted sensitive profiling of exosomal proteins for rapid cancer diagnosis

Sun

et al. 2020

Theranostics

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The proteins expressed on exosomes have emerged as promising liquid-biopsy biomarkers for cancer diagnosis. However, molecular profiling of exosomal proteins remains technically challenging. Herein, we report a nanozyme-assisted immunosorbent assay (NAISA) that enables sensitive and rapid multiplex profiling of exosomal proteins. This NAISA system is based on the installation of peroxidase-like nanozymes onto the phospholipid membranes of exosomes, thus avoiding the need for post-labelling detection antibodies. The exosomal proteins are determined by a sensitive nanozyme-catalyzed colorimetric assay less than 3 h, without the need for multi-step incubation and washing operations. Using NAISA to profile exosomal proteins from different cell lines and clinical samples, we reveal that tumor-associated exosomal proteins can serve as promising biomarkers for accurate cancer diagnosis in a cooperative detection pattern. Methods: Exosomes were engineered with DSPE-PEG-SH through hydrophobic interaction, and then were assembled with gold nanoparticles (2 nm) to produce Exo@Au nanozyme. The proteins on Exo@Au could be selectively captured by their specific antibodies seeded into a 96-well plate. The immobilized Exo@Au shows peroxidase-like activity to perform colorimetric assays by reaction with 3,3′,5,5′-tetramethylbenzidine (TMB) and H 2 O 2 . The protein levels of exosomes were recorded on a microplate reader. Results: The NAISA platform is capable of profiling multiple exosomal proteins from both cancer cell lines and clinical samples. The expression levels of exosomal proteins, such as CD63, CEA, GPC-3, PD-L1 and HER2, were used to classify different cancer cell lines. Moreover, the protein profiles have been applied to differentiate healthy donors, hepatitis B patients, and hepatic cell carcinoma (HCC) patients with high accuracy. Conclusion: The NAISA nanozyme was allowed to rapidly profile multiple exosomal proteins and could have great promise for early HCC diagnosis and identification of other cancer types.

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Section: Methodsmentioning

confidence: 99%

Nanozyme-assisted sensitive profiling of exosomal proteins for rapid cancer diagnosis

Sun

et al. 2020

Theranostics

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“…Another strategy for enhancing photothermally active PBnp with magnetic properties is the doping of the nanoparticles with the well-known MRI positive contrast agent gadolinium [ 69 ]. potassium/gadolinium hexacyanoferrate is simply synthesized mixing a Gd 3+ salt with Fe 3+ chloride, thus partially substituting iron(III) ions with gadolinium.…”

Section: Photothermal Properties Of Pbnp and Their Biomedical Usementioning

confidence: 99%

Prussian Blue Nanoparticles as a Versatile Photothermal Tool

2018

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Prussian blue (PB) is a coordination polymer studied since the early 18th century, historically known as a pigment. PB can be prepared in colloidal form with a straightforward synthesis. It has a strong charge-transfer absorption centered at ~700 nm, with a large tail in the Near-IR range. Irradiation of this band results in thermal relaxation and can be exploited to generate a local hyperthermia by irradiating in the so-called bio-transparent Near-IR window. PB nanoparticles are fully biocompatible (PB has already been approved by FDA) and biodegradable, this making them ideal candidates for in vivo use. While papers based on the imaging, drug-delivery and absorbing properties of PB nanoparticles have appeared and have been reviewed in the past decades, a very recent interest is flourishing with the use of PB nanoparticles as photothermal agents in biomedical applications. This review summarizes the syntheses and the optical features of PB nanoparticles in relation to their photothermal use and describes the state of the art of PB nanoparticles as photothermal agents, also in combination with diagnostic techniques.

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“…For example, gadolinium ions embedded in Prussian blue nanoparticles with a concentration gradient were demonstrated to facilitate proton relaxivity and magnetic resonance imaging contrast. 5 Similarly, a core of (Rb 0.5 Co[Fe(CN) 6 ] 0.8 ·H 2 O, RbCoFe) and a shell of (R b0.2 Ni[Cr(CN) 6 ] 0.7 ·H 2 O, RbNiCr) were successfully designed as photostrictive/piezo-magnetic heterostructures due to confinement effects. 6 In addition, the concentration gradient or core/shell structure was usually proposed to inhibit the mechanical degradation of core materials and improve the electrochemical cyclability of cathodes.…”

Section: Introductionmentioning

confidence: 99%

How Does Ion Exchange Construct Binary Hexacyanoferrate? A Case Study

2022

ACS Omega

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In this work, using electrochemical active Fe as an ion-exchange element (attack side) and the Na x MnFe(CN) 6 slurry with a high solid content (MnHCF) as a template (defensive side), a series of binary hexacyanoferrates are prepared via a simple Mn/Fe ion-exchange process, in which Na x FeFe(CN) 6 (FeHCF) and solid solution Na x (FeMn)Fe(CN) 6 are concentrated on the shell and the core, respectively. The proportions of the two structures are mainly controlled by the competition between the ion-exchange rate in the bulk material and dissolution-reprecipitation rate. Slowing down the attacking rate, such as the use of a chelating agent complexed with the attacker Fe, is advantageous to form a thermodynamically metastable state with homogeneous distribution of elements since the diffusion of Fe 2+ in the solid MnHCF is relatively fast. The shell FeHCF could be adjusted by the dissolution-reprecipitation rate, which is driven by the solubility difference. Adding the chelating agent in the defensive side will promote the dissolution of MnHCF and reprecipitation of FeHCF on the surface. Meanwhile, with the increase of Fe sources, the thickness of the shell FeHCF increases, and correspondingly the content of solid solution decreased due to FeHCF is more stable than solid solutions in thermodynamics. Finally, such a design principle in this case study could also be generalized to other ion-exchange processes. Considering the difference of two components in solubility, the larger difference can make the core/shell structure more clear due to the enhancement of dissolution–reprecipitation route.

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One-step synthesis of gradient gadolinium ironhexacyanoferrate nanoparticles: a new particle design easily combining MRI contrast and photothermal therapy

Cited by 41 publications

References 41 publications

Nanozyme-assisted sensitive profiling of exosomal proteins for rapid cancer diagnosis

Nanozyme-assisted sensitive profiling of exosomal proteins for rapid cancer diagnosis

Prussian Blue Nanoparticles as a Versatile Photothermal Tool

How Does Ion Exchange Construct Binary Hexacyanoferrate? A Case Study

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