One-step purification and oriented attachment of protein A on silica and graphene oxide nanoparticles using sortase-mediated immobilization

Qafari, Seyed Mehdi; Ahmadian, Gholamreza; Mohammadi, Mehdi

doi:10.1039/c7ra12128h

Cited by 12 publications

(17 citation statements)

References 46 publications

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“…A potential solution to overcome this problem, which has been developed in our lab, is the insertion of a sortase recognition motif (LPXTG) into different parts of VLP that are likely to be exposed on the particle surface. In this way, it is possible to retain the Tumor therapy [213] Rotavirus [66] Doxorubicin (DOX) Tumor therapy [214] Cowpea mosaic virus (CPMV) Doxorubicin (DOX) Tumor therapy [215] Cucumber mosaic virus (CMV) Doxorubicin (Dox) Tumor therapy [216] Hepatitis B virus (HBV) siRNA Tumor therapy [217] Polyomavirus Methotrexate (MTX) Tumor therapy [218] Filamentous bacteriophages (fd or M13) Chloramphenicol Antimicrobial drug [219] Bacteriophage Qβ Azithromycin/clarithromycin Antimicrobial drug [220] integrity of the VLP and make it possible to conjugate any protein on the VLP surface [221][222][223]. Other application for changing the external surface of VLPs is delivering the drug to a specific cell or tissue to treat a specific disease.…”

Section: Discussionmentioning

confidence: 99%

Virus-like particles: preparation, immunogenicity and their roles as nanovaccines and drug nanocarriers

et al. 2021

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Virus-like particles (VLPs) are virus-derived structures made up of one or more different molecules with the ability to self-assemble, mimicking the form and size of a virus particle but lacking the genetic material so they are not capable of infecting the host cell. Expression and self-assembly of the viral structural proteins can take place in various living or cell-free expression systems after which the viral structures can be assembled and reconstructed. VLPs are gaining in popularity in the field of preventive medicine and to date, a wide range of VLP-based candidate vaccines have been developed for immunization against various infectious agents, the latest of which is the vaccine against SARS-CoV-2, the efficacy of which is being evaluated. VLPs are highly immunogenic and are able to elicit both the antibody- and cell-mediated immune responses by pathways different from those elicited by conventional inactivated viral vaccines. However, there are still many challenges to this surface display system that need to be addressed in the future. VLPs that are classified as subunit vaccines are subdivided into enveloped and non- enveloped subtypes both of which are discussed in this review article. VLPs have also recently received attention for their successful applications in targeted drug delivery and for use in gene therapy. The development of more effective and targeted forms of VLP by modification of the surface of the particles in such a way that they can be introduced into specific cells or tissues or increase their half-life in the host is likely to expand their use in the future. Recent advances in the production and fabrication of VLPs including the exploration of different types of expression systems for their development, as well as their applications as vaccines in the prevention of infectious diseases and cancers resulting from their interaction with, and mechanism of activation of, the humoral and cellular immune systems are discussed in this review.

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Section: Discussionmentioning

confidence: 99%

Virus-like particles: preparation, immunogenicity and their roles as nanovaccines and drug nanocarriers

et al. 2021

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“…In this study, PA was immobilized not only at the fiber surface but in the 3D hydrogel shell-layer of PVA, which has the advantage that the diffusion length between PA and the antibody is lower than the 2D surface and the antibody could access PA from any direction readily [ 36 ]. To improve the accessibility of the antibody to PA, various site-specific immobilization methods, such as cysteine–maleimide reaction [ 37 ], peptide-tag conjugation [ 38 ], click-chemistry [ 39 ], and enzyme-mediated immobilization [ 40 ], would be applicable. In addition, the antibody used here was a wild type, but various recombinant PAs which are commercially available to improve their performance are also applicable.…”

Section: Discussionmentioning

confidence: 99%

One-Step Surface Immobilization of Protein A on Hydrogel Nanofibers by Core-Shell Electrospinning for Capturing Antibodies

Naganuma

Moriyama

Suye

et al. 2021

IJMS

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Nanofibers (NFs) are potential candidates as filter materials for affinity separation owing to their high liquid permeability based on their high porosity. Multiple and complex processes were conventionally performed to immobilize proteins for modifying NF surfaces. A simple method must be developed to immobilize proteins without impairing their biological activity. Herein, we succeeded in fabricating NFs with a core of cellulose acetate and a shell of hydrophilic polyvinyl alcohol immobilized with staphylococcal recombinant protein A by a one-step process based on core-shell electrospinning. A total of 12.9 mg/cm3 of antibody was captured in the fiber shell through high affinity with protein A immobilized in an aqueous environment of the hydrogel. The maximum adsorption site and dissociation constant evaluated by the Langmuir model were 87.8 µg and 1.37 µmol/L, respectively. The fiber sheet withstood triplicate use. Thus, our NF exhibited high potential as a material for membrane chromatography.

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“…The purified SpA fraction derived from high water/low acetonitrile solvent elution is the correctly folded form of SpA which retains the desired bioactivity. [13,37] Gel filtration Expensive [38] Polyhistidine affinity tag (His-tag) Affects expression levels. [12,37] Affinity chromatography The reagents used in the affinity chromatography technique are and have lower binding capacity.…”

Section: Per Aqueous Liquid Chromatography (Palc)mentioning

confidence: 99%

“…Given that SpA is the protein of choice for antibody purification with high purity and efficiency, which is an important factor to reduce time and cost; therefore, it can be used in industrial application of antibody-based anticancer drugs [37]. Recently, SpA was attached covalently on the surface of graphene oxide nanoparticles using sortase-mediated immobilization with some modifications and improvements and the immobilized SpA was able to efficiently purify IgG from the serum [38]. SpA can also be immobilized covalently on a polymeric support using enzymatic methods.…”

Section: Immobilization Of Spa On Solid Supportsmentioning

confidence: 99%

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A comprehensive review on staphylococcal protein A (SpA): Its production and applications

Rigi

Ghaedmohammadi

Ahmadian

2019

Biotech and App Biochem

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The Staphylococcus aureus protein A (SpA) can be obtained through the culture of wild-type S. aureus and also as a recombinant protein in safe bacterial hosts. Several methods have been used to purify SpA among which ion-exchange chromatography, affinity chromatography, gel filtration, and per aqueous liquid chromatography (PALC) are common. SpA has a wide range of biochemical, biotechnological, and medical applications and is most commonly used in test methods such as immunoprecipitation, enzyme-linked immunosorbent assay, and Western blotting. SpA has also been widely utilized in pharmaceutical applications to bind to immune complexes and serum immunoglobulins. SpA also directly binds to the B-cells preventing initiation of infectious diseases as well as having a role in the development of various autoimmune diseases. This review considers different applications of SpA in biotechnology and its novel clinical application for effective treatment of autoimmune diseases. It also discusses various strategies for expression and purification of the SpA including types of column chromatography that are commonly used in protein purification and developing SpA surface display technologies. Finally, this review highlights the potential and novel applications of SpA immobilization, SpA typing, protein engineering for further development of immunological and biochemical research, and also application of SpA as a diagnostic biosensor.

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One-step purification and oriented attachment of protein A on silica and graphene oxide nanoparticles using sortase-mediated immobilization

Abstract: One-step purification and oriented immobilization of protein A on functionalized carriers.

Cited by 12 publications

References 46 publications

Virus-like particles: preparation, immunogenicity and their roles as nanovaccines and drug nanocarriers

Virus-like particles: preparation, immunogenicity and their roles as nanovaccines and drug nanocarriers

One-Step Surface Immobilization of Protein A on Hydrogel Nanofibers by Core-Shell Electrospinning for Capturing Antibodies

A comprehensive review on staphylococcal protein A (SpA): Its production and applications

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