One-step generation of a targeted knock-in calf using the CRISPR-Cas9 system in bovine zygotes

Owen, Joseph R.; Hennig, Sadie L.; McNabb, Bret R.; Mansour, Tamer; Smith, Justin M.; Lin, Jason; Young, Amy; Trott, Josephine F.; Murray, James D.; Delany, Mary E.; Ross, Pablo J.; Eenennaam, Alison L. Van

doi:10.1186/s12864-021-07418-3

Cited by 20 publications

(9 citation statements)

References 40 publications

(54 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, this approach has its own shortcomings. It lacks full control over the time and extent of editing and can generate complex mosaic genotypes [ 36 , 37 ], which to a certain extent can be addressed by screening embryos prior to transfer [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Holstein Friesian dairy cattle edited for diluted coat color as a potential adaptation to climate change

et al. 2021

View full text Add to dashboard Cite

Background High-producing Holstein Friesian dairy cattle have a characteristic black and white coat, often with large proportions of black. Compared to a light coat color, black absorbs more solar radiation which is a contributing factor to heat stress in cattle. To better adapt dairy cattle to rapidly warming climates, we aimed to lighten their coat color by genome editing. Results Using gRNA/Cas9-mediated editing, we introduced a three bp deletion in the pre-melanosomal protein 17 gene (PMEL) proposed as causative variant for the semi-dominant color dilution phenotype observed in Galloway and Highland cattle. Calves generated from cells with homozygous edits revealed a strong color dilution effect. Instead of the characteristic black and white markings of control calves generated from unedited cells, the edited calves displayed a novel grey and white coat pattern. Conclusion This, for the first time, verified the causative nature of the PMEL mutation for diluting the black coat color in cattle. Although only one of the calves was healthy at birth and later succumbed to a naval infection, the study showed the feasibility of generating such edited animals with the possibility to dissect the effects of the introgressed edit and other interfering allelic variants that might exist in individual cattle and accurately determine the impact of only the three bp change.

show abstract

Section: Discussionmentioning

confidence: 99%

Holstein Friesian dairy cattle edited for diluted coat color as a potential adaptation to climate change

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The recent increase in CRISPR/Cas9 use has led to the production of specific gene-targeted cattle [ 62 64 ]. Even in cattle, germline transmission of the specific gene mutation was proven.…”

Section: Genomic Selectionmentioning

confidence: 99%

Outlook on genome editing application to cattle

Gim,

Jang

2024

J Vet Sci

View full text Add to dashboard Cite

In livestock industry, there is growing interest in methods to increase the production efficiency of livestock to address food shortages, given the increasing global population. With the advancements in gene engineering technology, it is a valuable tool and has been intensively utilized in research specifically focused on human disease. In historically, this technology has been used with livestock to create human disease models or to produce recombinant proteins from their byproducts. However, in recent years, utilizing gene editing technology, cattle with identified genes related to productivity can be edited, thereby enhancing productivity in response to climate change or specific disease instead of producing recombinant proteins. Furthermore, with the advancement in the efficiency of gene editing, it has become possible to edit multiple genes simultaneously. This cattle breed improvement has been achieved by discovering the genes through the comprehensive analysis of the entire genome of cattle. The cattle industry has been able to address gene bottlenecks that were previously impossible through conventional breeding systems. This review concludes that gene editing is necessary to expand the cattle industry, improving productivity in the future. Additionally, the enhancement of cattle through gene editing is expected to contribute to addressing environmental challenges associated with the cattle industry. Further research and development in gene editing, coupled with genomic analysis technologies, will significantly contribute to solving issues that conventional breeding systems have not been able to address.

show abstract

“…From these gene editing results in cattle, it has been demonstrated that the MSTN mutation is transmitted to the next generation through the germline, laying the foundation for the mass production of MSTN mutation cattle ( 52 ). On the other hand, for only males to be born, an in vitro fertilized bovine embryo, in which the SRY gene was inserted into chromosome 17 using CRISPR/Cas9, was produced, and a report was published in 2021 that a calf was successfully born ( 56 ).…”

Section: Application Of Genome-engineered Livestock ( Fig 3...mentioning

confidence: 99%

Current status and future of gene engineering in livestock

Kwon,

Gim,

Yum

et al. 2024

BMB Rep

View full text Add to dashboard Cite

The application of gene engineering in livestock is necessary for various reasons, such as increasing productivity and producing disease resistance and biomedicine models. Overall, gene engineering provides benefits to the agricultural and research aspects, and humans. In particular, productivity can be increased by producing livestock with enhanced growth and improved feed conversion efficiency. In addition, the application of the disease resistance models prevents the spread of infectious diseases, which reduces the need for treatment, such as the use of antibiotics; consequently, it promotes the overall health of the herd and reduces unexpected economic losses. The application of biomedicine could be a valuable tool for understanding specific livestock diseases and improving human welfare through the development and testing of new vaccines, research on human physiology, such as human metabolism or immune response, and research and development of xenotransplantation models. Gene engineering technology has been evolving, from random, time-consuming, and laborious methods to specific, time-saving, convenient, and stable methods. This paper reviews the overall trend of genetic engineering technologies development and their application for efficient production of genetically engineered livestock, and provides examples of technologies approved by the United States (US) Food and Drug Administration (FDA) for application in humans.

show abstract

One-step generation of a targeted knock-in calf using the CRISPR-Cas9 system in bovine zygotes

Cited by 20 publications

References 40 publications

Holstein Friesian dairy cattle edited for diluted coat color as a potential adaptation to climate change

Holstein Friesian dairy cattle edited for diluted coat color as a potential adaptation to climate change

Outlook on genome editing application to cattle

Current status and future of gene engineering in livestock

Contact Info

Product

Resources

About