One-pot synthesized DNA-templated Ag/Pt bimetallic nanoclusters as peroxidase mimics for colorimetric detection of thrombin

Zheng, Cheng; Zheng, Aixian; Liao, Bo; Zhang, Xiaolong; He, Yu; Li, Juan; Yang, Huang‐Hao

doi:10.1039/c4cc05339g

Cited by 118 publications

(46 citation statements)

References 40 publications

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“…The intrinsic fluorescence of metal NCs, which has been discussed in Section , can be readily used as an optical signal to sense the interactions of metal NCs with the biological species of interests. A variety of configurations exist to use the optical properties of metal NCs, ranging from colorimetric change, direct observation of fluorescence color change by naked eyes upon UV illumination, fluorescence enhancement (‘turn‐on’ assays) or fluorescence attenuation (‘turn‐off’ assays) . In this section, we will discuss the sensing mechanisms in different configurations, with a focus on identifying the prevailing recognition elements for biological sensing.…”

Section: Desirable Features Of Metal Ncs For Theranostic Applicationsmentioning

confidence: 99%

Rational Design of Biomolecular Templates for Synthesizing Multifunctional Noble Metal Nanoclusters toward Personalized Theranostic Applications

Mok

Loh

et al. 2016

Adv Healthcare Materials

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thiolate-protected, [ 3 ] protein/peptide-templated, [ 4 ] and DNA-templated [ 5 ] ones. The last two types of metal NCs use biomolecules as templates to direct the synthesis (thus termed as biotemplated metal NCs), which afford more attractive features such as good aqueous solubility, superb biocompatibility, and versatile surface chemistries, [ 6 ] in addition to their ultrasmall size and superior photoluminescent optical properties. These desirable features render biotemplated metal NCs as promising candidates for various biomedical applications like imaging, [ 7 ] sensing, [ 7b,8 ] delivery [ 9 ] and therapeutics. [ 10 ] Theranostics is a revolutionary treatment strategy proposed by Funkhouser in 1998, which combines accurate diagnosis and targeted therapeutics. [ 11 ] From the list of individal biomedical applications mentioned earlier, a combination of sensing and imaging modalities would be fundamental for disease diagnosis. [ 12 ] In the same vein, image-guided targetedtherapy allows for real-time in vivo monitoring of the pharmacokinetics and pharmacodynamics of the therapeutic agent to enhance treatment effi ciency. [ 13 ] Through the ingenious combination of selective modalities, a highly potent and personalized theranostic agent could be developed. In particular, NC-based systems are poised to be the next versatile theranostic platform. This is because the intrinsic photoluminescence of the noble metal core can be harnessed for fl uorescence imaging, and tailor-made to exhibit unique optical properties for therapy, such as the generation of singlet oxygen [ 14 ] to substitute organicbased photosensitizers for possible photodynamic cancer treatments. In addition to the metal core, the biomolecular ligand shell surrounding the metal NC core can be easily functionalized with biorecognition moieties for sensing and targeted delivery. Due to the large surface to volume ratio of metal NCs, it can also serve as a reservoir sink to load multiple drugs/ genes and be modifi ed with sensing/imaging agents to imbue both therapeutic and diagnostic functions.The selective marriage of respective modalities to form theranostic biotemplated metal NCs will have a far-reaching impact on the future of medicine and healthcare. There are large amounts of work that use randomly picked natural biomolecules as templates to synthesize metal NCs for biomedical

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Section: Desirable Features Of Metal Ncs For Theranostic Applicationsmentioning

confidence: 99%

Rational Design of Biomolecular Templates for Synthesizing Multifunctional Noble Metal Nanoclusters toward Personalized Theranostic Applications

Mok

Loh

et al. 2016

Adv Healthcare Materials

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“…TEM image showed that the size of the DNA−Au/Pt NCs was about 2 to 4 nm (Figure ), which was in agreement with previous report by Zheng 2014. The difference in size was probably due to the different lengths of oligonucleotide template . Also, the size distribution measured by DLS revealed that approximately over 60% of particles are between 5 to 7 nm.…”

Section: Resultsmentioning

confidence: 99%

“…Su et al., with help of peroxidase property of Au@Pt nanoparticles designed a colorimetric biosensor for tracing Escherichia coli O157:H7 . Conjugation of peroxidase mimic nanostructure with bio‐receptor not only decreased catalytic activity but also diminish binding affinity of bio‐receptor . Nowadays DNA has been attractive for its potency as a scaffold for synthesis of nanoclusters .…”

Section: Introductionmentioning

confidence: 99%

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New Colorimetric DNA Sensor for Detection of Campylobacter jejuni in Milk Sample Based on Peroxidase‐Like Activity of Gold/Platinium Nanocluster

et al. 2019

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A new colorimetric DNA sensor for trace Campylobacter jejuni DNA genome in food samples was investigated. 30 mer DNA probe‐based on hipo gene of Campylobacter jejuni and 12 cytosine nucleotide in tail without any chemical modification was used as the sensing element. 12 cytosine nucleotide utilized as a scaffold for stabilization of Au/Pt nanoclusters. Peroxidase like activity of Au/Pt nanocluster probe with the addition of bacterial target DNA and hybridization process decreased. The experiment showed that step by step increase in bacterial target DNA gradually decreased peroxidase activity, which was linear. Limit of detection for this bacterial DNA sensor was 20 pM and superiority of this DNA sensor for detection of Campylobacter jejuni DNA in comparison with other target DNA was significant. The method was successfully applied to the analysis of bacterial target DNA in milk samples, with results similar to those obtained by the PCR methodology as comparative method.

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“…Enzyme-like nanomaterials (nanozymes) [1,2] based on metallic [3][4][5], metal oxide [6][7][8][9][10], bimetallic nanoparticles (NPs) and nanoclusters (NCs) [11][12][13][14], carbon nanomaterials [15][16][17], and hybrid nanocomposites [18][19][20][21][22][23][24][25], have recently received a great deal of attention for their biochemical applications. Enzyme-mimicking molecules (e.g., metal complexes, hemin and other porphyrins, cyclodextrins, and supramolecules) also have been reported to have many advantages over natural enzymes [26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Self-templated formation of aptamer-functionalized copper oxide nanorods with intrinsic peroxidase catalytic activity for protein and tumor cell detection

Harroun

Lien

et al. 2016

Sensors and Actuators B: Chemical

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One-pot synthesized DNA-templated Ag/Pt bimetallic nanoclusters as peroxidase mimics for colorimetric detection of thrombin

Cited by 118 publications

References 40 publications

Rational Design of Biomolecular Templates for Synthesizing Multifunctional Noble Metal Nanoclusters toward Personalized Theranostic Applications

Rational Design of Biomolecular Templates for Synthesizing Multifunctional Noble Metal Nanoclusters toward Personalized Theranostic Applications

New Colorimetric DNA Sensor for Detection of Campylobacter jejuni in Milk Sample Based on Peroxidase‐Like Activity of Gold/Platinium Nanocluster

Self-templated formation of aptamer-functionalized copper oxide nanorods with intrinsic peroxidase catalytic activity for protein and tumor cell detection

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