On Total Hemolymph (Blood) Cell Counts of Insects I. Orthoptera, Odonata, Hemiptera, and Homoptera

Tauber, Oscar E.; Yeager, J. Franklin

doi:10.1093/aesa/28.2.229

Cited by 52 publications

(16 citation statements)

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“…At least 3 drops from each insect were counted and 5 replicates for different insect stages at different time intervals. The above method was proposed by Tauber and Yeager (1935). The number of haemocytes per cubic millimeter was calculated according to the equation of Jones (1962):…”

Section: Total Haemocyte Counts (Thcs)mentioning

confidence: 99%

Qualitative and Quantitative Changes of Haemocytes from Honeybee, Apis mellifera (L.) (Hymenoptera: Apidae) After Intrahaemocoelic Injection with Bacillus thuringiensis

Barakat¹,

Moselhy²,

Shouaib³

et al. 2017

GJBS

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The present work was carried out aiming to characterize the haemocytes from different developmental stages of honeybee, Apis mellifera (L.) after experimental injection of Bacillus thuringiensis (B.t.) into the haemocoel. Six haemocyte types were recognized in pupae: prohaemocytes (PRs), granulocytes (GRs), oenocytoides (OEs), spindle-shaped cells (SPLs), plasmatocytes (PLs), and adipohaemocytes (ADs). Eight types were found in larvae; in addition to the previous mentioned haemocyte types, spherulocytes (SPs) and eosinophil cells (EOs), while ten types were recognized in the adult stage; the previous mentioned eight types plus micronucleocytes (MIs) and macronucleocytes (MAs). Due to bacterial injection, certain pathological consequences were observed in the defined haemocytes such as vacuolization in the cytoplasm, distortion of the cell membrane and pycnosis in the nuclei. Moreover, the differential and total haemocyte counts were found to increased or decreased depending on the developmental stage and the time of haemolymph collection after bacterial injection. Other observed cellular responses against bacterial injection were phagocytosis and nodule formation.

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Section: Total Haemocyte Counts (Thcs)mentioning

confidence: 99%

Qualitative and Quantitative Changes of Haemocytes from Honeybee, Apis mellifera (L.) (Hymenoptera: Apidae) After Intrahaemocoelic Injection with Bacillus thuringiensis

Barakat¹,

Moselhy²,

Shouaib³

et al. 2017

GJBS

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“…Samples were centrifuged at 20000rpm for 10 min. The supernatant was used to estimate Aspartate transaminase (AST) and Alanine transaminase (ALT) according to 18 whereas Protein carbonyl content by 19 . For haemocyte estimation, haemolymph was drawn into a Thoma white blood cell pipette up to 0.5 mark and diluted up to the 11 mark with Tauber-yeager fluid 20 .…”

Section: Methodsmentioning

confidence: 99%

Untitled

2016

IJPSR

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Bacterial diseases are highly virulent and cause significant crop loss in Anthereae mylittadrury (Daba TV).Therefore an attempt has been made to evaluate the efficient concentration of sodium hypochlorite in controlling bacterial infection through studies on mortality, Total haemocytes, Protein carbonyl content, Alanine transaminase, Aspartate transaminase in the fifth in star larvae. Results reveal that supplementation of 0.05% sodium hypochlorite concentration had significantly reduced the mortality rate due to bacterial infections. In comparison with the control (14788±123), the haemocyte count in 0.05% sodium hypochlorite supplemented larvae were recorded low as14125±146 whereas 0.1% and 0.01%concentration supplementation resulted in an increase of 15025±158 and 14965±185 haemocytes. In comparison with the control results also show that protein carbonyl content was doubled with 0.05% supplementation whereas an increase of 33.3% and 16.6% was recorded with 0.01% and 0.1% respectively. Alanine aminotransferase had shown significant increase of 138.7% with 0.05% sodium hypochlorite supplementation followed by 39% increase with 0.01% concentration in its activity. Supplementation with 0.1% did not show much variation in Alanine aminotransferase from the control. Aspartate aminotransferase activity levels found increased by 106.2% in fifth in star larvae supplemented with 0.05% sodium hypochlorite whereas 30.2% and 21.75% increase was recorded with 0.01%, 0.1% respectively. Based on the results obtained from present study 0.05% sodium hypochlorite is found efficient in controlling bacterial infections in Anthereae mylitta drury (Daba TV).

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“…The number of haemocytes in the haemolymph of unfixed insects is always lower in comparison to that in the haemolymph of a fixed insect (Tauber & Yeager 1935). The reason for such variation is the adhesion and coagulation of the haemocytes at the site of withdrawal.…”

Section: Total Haemocyte Count (Thc)mentioning

confidence: 97%

“…Furthermore, the THC variations reported may be dependent on different techniques. Tauber and Yeager (1934) initiated an investigation of THC in insects belonging to Orthoptera, Odonata, Hemiptera and Homoptera (Tauber & Yeager 1935) as well as Neuroptera, Coleoptera, Lepidoptera and Hymenoptera (Tauber & Yeager 1936). According to them, the THC in the nymphs of hemimetabolous insects was lower compared to those of the respective adults.…”

Section: Total Haemocyte Count (Thc)mentioning

confidence: 99%

Review of haemocyte count, response to chemicals, phagocytosis, encapsulation and metamorphosis in insects

Siddiqui

Al‐Khalifa

2013

Italian Journal of Zoology

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On Total Hemolymph (Blood) Cell Counts of Insects I. Orthoptera, Odonata, Hemiptera, and Homoptera

Cited by 52 publications

References 0 publications

Qualitative and Quantitative Changes of Haemocytes from Honeybee, Apis mellifera (L.) (Hymenoptera: Apidae) After Intrahaemocoelic Injection with Bacillus thuringiensis

Qualitative and Quantitative Changes of Haemocytes from Honeybee, Apis mellifera (L.) (Hymenoptera: Apidae) After Intrahaemocoelic Injection with Bacillus thuringiensis

Untitled

Review of haemocyte count, response to chemicals, phagocytosis, encapsulation and metamorphosis in insects

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