On the Total Hemolymph (Blood) Cell Counts of Insects II. Neuroptera, Coleoptera, Lepidoptera and Hymenoptera

Tauber, Oscar E.; Yeager, J. Franklin

doi:10.1093/aesa/29.1.112

Cited by 30 publications

(10 citation statements)

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“…Variation in cell count arises depending on method of studying haemocytes, examination of fixed and unfixed haemocytes ( Rosenberger and Jones, 1960 ) and age difference ( Gilliam and Shimanuki, 1967 ). Total haemocyte counts (THC) ranging from 15,000 to 275,000 cells/mm 3 were observed in Gryllus assimilis and 15000–60000 cells/mm 3 in Periplaneta ( Tauber and Yeager, 1934 , Tauber and Yeager, 1935 , Tauber and Yeager, 1936 ) which were later counted as 26050–42250 cells/mm 3 in males and 29000–46600 cells/mm 3 in females of Gryllus bimaculatus ( Mahmood and Yousuf, 1985 ). Gupta and Sutherland (1968) later counted THC in P. americana from 7996 to 27796 cells/mm 3 .…”

Section: Discussionmentioning

confidence: 99%

Toxicity of some insecticides to the haemocytes of giant honeybee, Apis dorsata F. under laboratory conditions

Perveen

Ahmad

2017

Saudi Journal of Biological Sciences

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Quantitative studies concerning total and differential haemocyte counts and abnormalities were performed under laboratory conditions for larvae, pupae and adults collected from a wild colony. Haemolymph samples were observed immediately, thirty and sixty minutes after field recommended concentration exposure of five different insecticides. Total haemocyte counts were significantly higher for larvae and pupae but less for adult bees, however, differential haemocyte counts insignificantly different. Exposure of insecticides showed variable response for tested insecticides with immediate increased change with ethofenprox, diafenthiuron and imidacloprid but decreased for all tested insecticides after sixty minutes. For differential haemocyte counts, plasmatocytes and granulocytes increased with exposure of insecticides. Immune response of haemocytes against insecticides showed different degrees of abnormalities like agglutination, denucleation and cell shape distortion. Such studies may help in possible identification of insect defense mechanisms against their exposure to external hazards for instance insecticide exposure.

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Section: Discussionmentioning

confidence: 99%

Toxicity of some insecticides to the haemocytes of giant honeybee, Apis dorsata F. under laboratory conditions

Perveen

Ahmad

2017

Saudi Journal of Biological Sciences

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“…Des dicomptes diffkrentiels d6taillCs des cellules de I'htmolymphe des Noctuidae et des Pyralididae ont t t t publiCs par Yeager (1945) et Arnold (1952) tandis que plusieurs travaux se sont attardCs B la simple description des types htmocytaires de diffkrentes familles (Akai et Sato 1973;Lai-Fook 1973;Neuwirth 1973;Arnold et Sohi 1974). Plusieurs auteurs, dont Jones (1959), ont donne les descriptions des htmocytes et ont effectut le comptage diffkrentiel; trts souvent, seul le comptage de un ou deux des stades post-embryonnaires de l'insecte est effectui (Tauber et Yeager 1936;Rosenberger et Jones 1960;Stephens 1963;Shrivastava et Richards 1965;Dahlman 1972). Les reactions histochimiques des differents types cellulaires ont &ti ttudites par Asshurst et Richards (1964) ainsi que par tous ceux qui se sont specialement attardCs aux descriptions cellulaires.…”

Section: Introductionunclassified

ÉTUDE DU COMPLEXE HÉMOCYTAIRE POSTEMBRYONNAIRE DE LAMBDINA FISCELLARIA FISCELLARIA (LEPIDOPTERA: GEOMETRIDAE)

Boiteau

Perron

1977

Can Entomol

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ResumeCan. Enr. 109: 269-276 (1977) Les auteurs ont etudit les htmocytes de Lambdinafiscellariaflscellaria (Guen.) maintenus en suspension ou enrobts dans l'tpon. Cinq types difftrents de cellules sanguines ont kt6 dtcrits.Ce sont les prohemocytes, les plasmatocytes, les htmocytes granulaires, les cellules i sphtrules et les oenocytoi'des. La faible importance numerique des prohtmocytes et des oenocytoi'des, la baisse significative du nombre des plasmatocytes pendant les derniers stades larvaires ainsi que la hausse correspondante des htmocytes granulaires et des cellules ? i sphirules sont discuttes au regard de la litttrature. Les comptages htmocytaire totaux ont tgalement t t t effectuts. AbstractHernocytes of Lambdina fiscellariafiscellaria (Guen.) were examined as suspended cells or as slides from a culot. Five types of hemocytes were identified: prohemocytes, plasmatocytes, granular hemocytes, spherule cells, and oenocytoids. The low numbers of prohemocytes and oenocytoids, the significant decrease in the number of plasmatocytes in last larval stages, and the corresponding increase in granular and spherule cells have been compared with existent literature. Total hemocyte counts were also made.

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“…The THC/mm 3 was calculated as described by Jones 68 . G. mellonella larvae were pierced on the first hind leg using a sterile needle, and 10 μL of hemolymph was withdrawn and mixed with 900 μL of Tauber-Yeager solution 69 . A drop of the diluted hemolymph was applied to a Neubauer hemocytometer.…”

Section: Methodsmentioning

confidence: 99%

Effects of Selenium and Vitamin E on Enzymatic, Biochemical, and Immunological Biomarkers in Galleria mellonella L

Coşkun

Kayış

Gulsu

et al. 2020

Sci Rep

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To understand the effects of micronutrients have particular biological functions that are involved mainly in the antioxidant system, which has essential implications for the development of diseases, this study investigated how vitamin E, selenium, and their combination affect lipid, protein, carbohydrate, and malondialdehyde (MDA) content; antioxidant enzyme (catalase [CAT], superoxide dismutase [SOD], glutathione-S-transferase [GST]) activity; and the total hemocyte count (THC) in larvae of Galleria mellonella L. fed different diets. Diet 1 (100 µg of selenium) significantly decreased carbohydrate and lipid content. Diets 2 (100 µg of vitamin E), 3 (100 µg of selenium and vitamin E each), and 5 (Tween 80) did not significantly affect protein and carbohydrate content. Diet 2 significantly increased the lipid content compared to diet 4 (control). Diet 1 increased CAT, SOD, and GST activity and MDA content (highest at 27.64 nmol/mg protein). Diet 2 significantly decreased SOD activity and MDA content compared to other diets. Diet 1 significantly decreased the THC compared to other diets. These results suggested that selenium changes oxidative stress parameters, energy reserves, and THC in G. mellonella. These changes could be a physiological adaptation against selenium-induced oxidative stress. Vitamin E could play a protective role in selenium toxicity. Trace elements play a significant role in many biochemical and physiological processes and therefore are essential for living organisms 1. Selenium (symbol Se), a trace element that is a major component of glutathione peroxidase, plays an important role in intracellular defense against reactive oxygen species (ROS), in addition to being essential for growth and development 2. However, high selenium levels can be toxic 3,4. The utility and toxicity of selenium depend on its chemical form and concentration; in its inorganic form (selenates, selenides, and selenite), selenium is more toxic and less useful compared to its organic forms (selenomethionine and selenocysteine). Excessive selenium levels have cytotoxic, genotoxic, and carcinogenetic effects due to ROS accumulation and increased oxidative stress 4,5. Cells use antioxidants to neutralize the harmful effects of ROS. Many of these antioxidants decrease DNA damage, decrease protein denaturation or loss, and prevent lipid peroxidation 6,7. In insects, ascorbate peroxidase, superoxide dismutase (SOD), catalase (CAT), peroxidase, glutathione-S-transferase (GST), ascorbic acid, and vitamin E are the most important components of the antioxidant defense mechanism 6. SOD is involved in the conversion of the superoxide radical (O 2 −) to H 2 O 2 , and the H 2 O 2 is eliminated by CAT and ascorbate peroxidase. GST is involved in eliminating H 2 O 2 from cells and lipid peroxidation agents 7,8 and also decreases the effects of toxins by binding xenobiotics to glutathione (GSH: γ-glutamyl-cysteinyl-glycine). GST is a member of the multifunctional phase II biotransformation multigene enzyme family that catalyzes the conjuga...

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On the Total Hemolymph (Blood) Cell Counts of Insects II. Neuroptera, Coleoptera, Lepidoptera and Hymenoptera

Cited by 30 publications

References 0 publications

Toxicity of some insecticides to the haemocytes of giant honeybee, Apis dorsata F. under laboratory conditions

Toxicity of some insecticides to the haemocytes of giant honeybee, Apis dorsata F. under laboratory conditions

ÉTUDE DU COMPLEXE HÉMOCYTAIRE POSTEMBRYONNAIRE DE LAMBDINA FISCELLARIA FISCELLARIA (LEPIDOPTERA: GEOMETRIDAE)

Effects of Selenium and Vitamin E on Enzymatic, Biochemical, and Immunological Biomarkers in Galleria mellonella L

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