On the Preparation, Characterization, and Enzymatic Activity of Fungal Protease−Gold Colloid Bioconjugates

Gole, Anand; Dash, Chandravanu; Soman, Chinmay; Sainkar, S. R.; Rao, M. Narayana; Sastry, Murali

doi:10.1021/bc0001241

Cited by 136 publications

(88 citation statements)

References 31 publications

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“…Although the linkage of sulphur-bearing groups to GNPs and also linking different ligands to them are very appealing, practical and useful [11][12][13][14][15], the amine groups binding to GNPs can be considered in the same level. In fact, the linkage of GNPs to alkyl amine can facilitate the transfer of GNPs to organic solvents [16], and also the strong linkage of amino groups in amino acids and proteins to GNPs surface are related to the medical application of these hybrid materials [17,18].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of capped gold nanoparticles by using various amino acids

Zare

Khoshnevisan

Barkhi

et al. 2013

Journal of Experimental Nanoscience

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The production of gold nanoparticles (GNPs) by amino acid is one of the most attractive and interesting subjects in nanobiotechnology. In this study, amino acids have been utilised as a reducing agent and also an agent for capping GNPs. The GNPs were prepared using a reduction solution containing gold cations with optimum concentration of gold salt (5 mM), and also functionalised by glutamic acid, phenylalanine and tryptophan with optimum concentration of amino acids (25 mM). The optimum condition of gold solution and amino acids were achieved by ultraviolet-visible spectroscopy. The size of nanoparticles was obtained 5-20, 10-20 and 20-30 nm, respectively, by transmission electron microscopy and dynamic light scattering techniques. The results obtained from experimental and quantum calculations confirm that amino acids have strong bond while they have anion binding. Moreover, the free carboxylic groups of capped GNPs are one of the suitable and capable beads for binding biological agents. As a result, the medical applications of amino acids and proteins can be used as a practical method due to the strong interaction of peripheral amine groups with nanoparticles.

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Section: Introductionmentioning

confidence: 99%

Fabrication of capped gold nanoparticles by using various amino acids

Zare

Khoshnevisan

Barkhi

et al. 2013

Journal of Experimental Nanoscience

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“…[29][30][31][32][33][34][35][36][37] Furthermore, NP-protein conjugates are used in many applications from sensing [38][39][40][41] and nanobiotechnology. [22,42] A number of experimental methods has been utilised in studying protein structures in solution [43] including circular dichroism, [44][45][46] Fourier transform infrared spectroscopy, [47][48][49] Raman spectroscopy/SERS [50,51] and fluorescence spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

The Influence of Surface Composition of Nanoparticles on their Interactions with Serum Albumin

et al. 2010

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Interactions between differently functionalised silver and gold nanoparticles (NPs) as well as polystyrene nanoparticles with bovine serum albumin (BSA) are studied using circular dichroism (CD) spectroscopy. It is found that the addition of NPs to the protein solution destroys part of the helical secondary structure of the protein as a result of surface adsorption. From the loss of free protein and hence the extent of their structural change adsorption equilibrium constants are derived. The results reveal that citrate‐coated gold and silver NPs exhibit much stronger interactions with BSA than polymeric or polymer‐coated metallic NPs. It is therefore concluded that for the particles considered, the influence of surface composition on the interaction behaviour dominates that of the core.

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“…40,41 Previous studies have proposed that protein-nanoparticle interactions can occur either through free amine groups or cysteine residues in proteins and via electrostatic attraction of negatively charged carboxylate groups in some enzymes. 37,42 The two bands observed at 1,380 cm -1 and 1,030 cm -1 can be assigned to the C-N stretching vibrations of the aromatic and aliphatic amines, respectively.…”

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confidence: 99%

Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells

Zhang¹,

Choi²,

Han³

et al. 2015

IJN

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Background Silver nanoparticles (AgNPs) possess unique physical, chemical, and biological properties. AgNPs have been increasingly used as anticancer, antiangiogenic, and antibacterial agents for the treatment of bacterial infections in open wounds as well as in ointments, bandages, and wound dressings. The present study aimed to investigate the effects of two different sizes of AgNPs (10 nm and 20 nm) in male somatic Leydig (TM3) and Sertoli (TM4) cells and spermatogonial stem cells (SSCs). Methods Here, we demonstrate a green and simple method for the synthesis of AgNPs using Bacillus cereus culture supernatants. The synthesized AgNPs were characterized using ultraviolet and visible absorption spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and transmission electron microscopy (TEM). The toxicity of the synthesized AgNPs was evaluated by the effects on cell viability, metabolic activity, oxidative stress, apoptosis, and expression of genes encoding steroidogenic and tight junction proteins. Results AgNPs inhibited the viability and proliferation of TM3 and TM4 cells in a dose- and size-dependent manner by damaging cell membranes and inducing the generation of reactive oxygen species, which in turn affected SSC growth on TM3 and TM4 as feeder cells. Small AgNPs (10 nm) were more cytotoxic than medium-sized nanoparticles (20 nm). TEM revealed the presence of AgNPs in the cell cytoplasm and nucleus, and detected mitochondrial damage and enhanced formation of autosomes and autolysosomes in the AgNP-treated cells. Flow cytometry analysis using Annexin V/propidium iodide staining showed massive cell death by apoptosis or necrosis. Real-time polymerase chain reaction and western blot analyses indicated that in TM3 and TM4 cells, AgNPs activated the p53, p38, and pErk1/2 signaling pathways and significantly downregulated the expression of genes related to testosterone synthesis (TM3) and tight junctions (TM4). Furthermore, the exposure of TM3 and TM4 cells to AgNPs inhibited proliferation and self-renewal of SSCs. Conclusion Our results suggest that AgNPs exhibit size-dependent nanoreprotoxicity in male somatic cells and SSCs, strongly suggesting that applications of AgNPs in commercial products must be carefully evaluated. Further studies of AgNPs-induced nanoreprotoxicity in animal models are required.

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On the Preparation, Characterization, and Enzymatic Activity of Fungal Protease−Gold Colloid Bioconjugates

Cited by 136 publications

References 31 publications

Fabrication of capped gold nanoparticles by using various amino acids

Fabrication of capped gold nanoparticles by using various amino acids

The Influence of Surface Composition of Nanoparticles on their Interactions with Serum Albumin

Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells

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