On the possibility of direct triplet state excitation of indole

Chavez, Jose; Ceresa, Luca; Kitchner, Emma; Kimball, Joseph; Shtoyko, Tanya; Fudala, Rafał; Borejdo, Julian; Gryczyński, Zygmunt; Gryczyński, Ignacy

doi:10.1016/j.jphotobiol.2020.111897

Cited by 13 publications

(10 citation statements)

References 24 publications

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“…Recently our research group, as well as others, investigated room temperature phosphorescence (RTP) from indole derivatives embedded in poly (vinyl alcohol) (PVA) films [1][2][3][4][5]. Interestingly, we were able to excite triplet states of these compounds directly with blue light.…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, we were able to excite triplet states of these compounds directly with blue light. An excitation with long-wavelength light avoids the involvement of the singlet excited state and results in high values of phosphorescence anisotropies [3,4]. Many studies have been performed on RTP of proteins utilizing the tryptophan (TRP) chromophore [6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

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Direct excitation of tryptophan phosphorescence. A new method for triplet states investigation

Chavez¹,

Ceresa²,

Reeks³

et al. 2022

Methods Appl. Fluoresc.

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We studied room temperature phosphorescence of tryptophan (TRP) embedded in poly (vinyl alcohol) films. With UV (285 nm) excitation, the phosphorescence spectrum of tryptophan appears at about 460 nm. We also observed the TRP phosphorescence with blue light excitation at 410 nm, well outside of the S0→S1 absorption. This excitation reaches the triplet state of tryptophan directly without the involvement of the singlet excited state. The phosphorescence lifetime of tryptophan is in the sub-millisecond range. The long-wavelength direct excitation to the triplet state results in high phosphorescence anisotropy which can be useful in macromolecule dynamics study via time-resolved phosphorescence.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Direct excitation of tryptophan phosphorescence. A new method for triplet states investigation

Chavez¹,

Ceresa²,

Reeks³

et al. 2022

Methods Appl. Fluoresc.

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“…Furthermore, the close-lying electronic excited states of indole indicate the importance of vibronic coupling in the excited state properties of the indole chromophore. Due to its interesting excited state properties, indole has been extensively studied both experimentally and theoretically [3,[81][82][83][84][85][86].…”

Section: Vibronic Spectroscopy 311 Vibronic Spectra Of Indole Using T...mentioning

confidence: 99%

High-resolution electronic and vibrational spectroscopy of small-to-medium sized molecules with ab initio potential energy surface

Ray

Koner²,

Mondal³

2023

Electron. Struct.

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Electronic and vibrational spectroscopic studies of molecules are of crucial importance to characterizing a molecule and detecting the molecular species in different environments. In this review article, we summarized some important theoretical methods to calculate high-resolution electronic spectra and ro-vibrational states for small molecular systems with the inclusion of vibronic and ro-vibrational couplings, respectively. We have also reviewed a number of theoretical studies exploring some interesting organic chromophores like indole, isoalloxazine, transition metal trifluoride CoF3 and NiF3, and molecular ions like protonated rare gases and azido ions. These studies involve the calculation of spectroscopic features based on analytical potential energy surfaces constructed using high-level ab initio energies. The topology of the potential energy surfaces has been explored for these selected systems. The vibronic spectra and ro-vibrational states calculated using various theoretical methods and their comparison to available experimental results are reported in this review.

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“…It is important to notice that in the range up to 500 nm, PVA presents some residual absorbance [4,23]. So, when considering the transmittance of the BQ film it is important to consider excitation beam attenuation and emission intensity attenuation due to the PVA absorbance as well.…”

Section: Thin Layer Samplesmentioning

confidence: 99%

“…Over the last few decades, many experimental adaptations have been proposed and tested to realize such a configuration. We have been developing various configurations for front-face excitation over the last 40 years [4,[22][23][24][25][26]. As discussed in detail by us [4], many experimental designs are convenient but frequently wrong.…”

Section: Introductionmentioning

confidence: 99%

On the origin and correction for inner filter effects in fluorescence. Part II: secondary inner filter effect -the proper use of front-face configuration for highly absorbing and scattering samples

Ceresa

Kimball

Chavez

et al. 2021

Methods Appl. Fluoresc.

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Fluorescence is an established technology for studying molecular processes and molecular interactions. More recently fluorescence became a leading method for detection, sensing, medical diagnostics, biotechnology, imaging, DNA analysis, and gene expression. Consequently, precise and accurate measurements in various conditions have become more critical for proper result interpretations. Previously, in Part 1, we discussed inner filter effect type I, which is a consequence of the instrumental geometrical sensitivity factor and absorption of the excitation. In this part, we analyze inner filter effect type II and discuss the practical consequences for fluorescence measurements in samples of high optical density (absorbance/scattering). We consider both the standard square and front-face experimental configurations, discuss experimental approaches to limit/mitigate the effect and discuss methods for correcting and interpreting experimental results.

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On the possibility of direct triplet state excitation of indole

Cited by 13 publications

References 24 publications

Direct excitation of tryptophan phosphorescence. A new method for triplet states investigation

Direct excitation of tryptophan phosphorescence. A new method for triplet states investigation

High-resolution electronic and vibrational spectroscopy of small-to-medium sized molecules with ab initio potential energy surface

On the origin and correction for inner filter effects in fluorescence. Part II: secondary inner filter effect -the proper use of front-face configuration for highly absorbing and scattering samples

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