On the nature of antibiotic binding sites in ribosomes

Mitochondria and Risk for Deafness

“…The 1555 position occurs in a region homologous to that which is known to be involved in aminoglycoside binding to the bacterial rRNA (Cundliffe, 1987;Hornig, Woolley, & Luhrmann, 1987;Moazed & Noller, 1987). This lends support to the notion that the hypersensitivity trait is the direct result of altered, and presumably increased, binding of aminoglycosides to the mitochondrial ribosome.…”

Mitochondria and Risk for Deafness

“…The 1555 position occurs in a region homologous to that which is known to be involved in aminoglycoside binding to the bacterial rRNA (Cundliffe, 1987;Hornig, Woolley, & Luhrmann, 1987;Moazed & Noller, 1987). This lends support to the notion that the hypersensitivity trait is the direct result of altered, and presumably increased, binding of aminoglycosides to the mitochondrial ribosome.…”

Affinity labeling of the virginiamycin S binding site on the bacterial ribosome

“…In our case, the virginiamycin S moieties respectively susceptible or unsusceptible to noninactivating modifications were previously identified. Involvement of the picolinyl ring (Figure 1) in fixation to ribosomes was supported by the spectrofluorometric studies of the virginiamycin S binding reaction to 50S (Parfait et al, 1978; Moureau et al, 1983;Di Giambattista et al, 1984, 1987 and by the fact that all known reported modifications of this part of the molecule abolished its antibiotic action. By contrast, the 4-oxopipecolinic acid component, which is replaced by some amino acids (aspartic acid in vernamycin C…”

Thiostrepton-induced gene expression in Streptomyces lividans

“…Thiostrepton recognizes and binds to a specific rRNA sequence in the absence of proteins (7,8 (Fig. 6).…”

Mitochondria and Risk for Deafness

“…The 1555 position occurs in a region homologous to that which is known to be involved in aminoglycoside binding to the bacterial rRNA (Cundliffe, 1987;Hornig, Woolley, & Luhrmann, 1987;Moazed & Noller, 1987). This lends support to the notion that the hypersensitivity trait is the direct result of altered, and presumably increased, binding of aminoglycosides to the mitochondrial ribosome.…”

Affinity labeling of the virginiamycin S binding site on the bacterial ribosome

“…In our case, the virginiamycin S moieties respectively susceptible or unsusceptible to noninactivating modifications were previously identified. Involvement of the picolinyl ring (Figure 1) in fixation to ribosomes was supported by the spectrofluorometric studies of the virginiamycin S binding reaction to 50S (Parfait et al, 1978; Moureau et al, 1983;Di Giambattista et al, 1984, 1987 and by the fact that all known reported modifications of this part of the molecule abolished its antibiotic action. By contrast, the 4-oxopipecolinic acid component, which is replaced by some amino acids (aspartic acid in vernamycin C…”

Thiostrepton-induced gene expression in Streptomyces lividans

“…Thiostrepton recognizes and binds to a specific rRNA sequence in the absence of proteins (7,8 (Fig. 6).…”

Mitochondria and Risk for Deafness

“…The 1555 position occurs in a region homologous to that which is known to be involved in aminoglycoside binding to the bacterial rRNA (Cundliffe, 1987;Hornig, Woolley, & Luhrmann, 1987;Moazed & Noller, 1987). This lends support to the notion that the hypersensitivity trait is the direct result of altered, and presumably increased, binding of aminoglycosides to the mitochondrial ribosome.…”

Affinity labeling of the virginiamycin S binding site on the bacterial ribosome

“…In our case, the virginiamycin S moieties respectively susceptible or unsusceptible to noninactivating modifications were previously identified. Involvement of the picolinyl ring (Figure 1) in fixation to ribosomes was supported by the spectrofluorometric studies of the virginiamycin S binding reaction to 50S (Parfait et al, 1978; Moureau et al, 1983;Di Giambattista et al, 1984, 1987 and by the fact that all known reported modifications of this part of the molecule abolished its antibiotic action. By contrast, the 4-oxopipecolinic acid component, which is replaced by some amino acids (aspartic acid in vernamycin C…”

Thiostrepton-induced gene expression in Streptomyces lividans

“…Thiostrepton recognizes and binds to a specific rRNA sequence in the absence of proteins (7,8 (Fig. 6).…”