On the interaction of non-thermal atmospheric pressure plasma with tissues

Kalghatgi, Sameer; Kelly, Crystal; Cerchar, Ekaterina; Sensenig, Rachel; Brooks, Ari D.; Fridman, Alexander; Morss-Clyne, A.; Azizkhan‐Clifford, Jane; Friedman, Gary D.

doi:10.1109/ppc.2009.5386317

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…24,28 To test the role of these ROS in non-thermal plasma-induced apoptosis, we pre-incubated melanoma cells for 1 h in 2.25 mM NAC, an intracellular scavenger of ROS, followed by treatment at a dose of 5 and 15 J/cm 2 . Apoptotic activity was assayed 24 h post-plasma treatment using Annexin-v/PI staining.…”

Section: Resultsmentioning

confidence: 99%

“…Both charged and neutral particles can lead to additional ROS production in the treated fluid. 24,28 Direct effects of non-thermal plasma treatment could include local heating caused by plasma streamers or UV radiation. Non-thermal atmospheric pressure DBD plasma is able to produce a large concentration of ROS in the extracellular media during treatment.…”

Section: Discussionmentioning

confidence: 99%

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RETRACTED ARTICLE: Non-thermal Plasma Induces Apoptosis in Melanoma Cells via Production of Intracellular Reactive Oxygen Species

et al. 2010

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Non-thermal atmospheric pressure dielectric barrier discharge (DBD) plasma may provide a novel approach to treat malignancies via induction of apoptosis. The purpose of this study was to evaluate the potential of DBD plasma to induce apoptosis in melanoma cells. Melanoma cells were exposed to plasma at doses that did not induce necrosis, and cell viability and apoptotic activity were evaluated by Trypan blue exclusion test, Annexin-V/PI staining, caspase-3 cleavage, and TUNEL® analysis. Trypan blue staining revealed that non-thermal plasma treatment significantly decreased the viability of cells in a dose-dependent manner 3 and 24 h after plasma treatment. Annexin-V/PI staining revealed a significant increase in apoptosis in plasma-treated cells at 24, 48, and 72 h post-treatment (p<0.001). Caspase-3 cleavage was observed 48 h post-plasma treatment at a dose of 15 J/cm2. TUNEL® analysis of plasma-treated cells demonstrated an increase in apoptosis at 48 and 72 h post-treatment (p<0.001) at a dose of 15 J/cm2. Pre-treatment with N-acetyl-L-cysteine (NAC), an intracellular reactive oxygen species (ROS) scavenger, significantly decreased apoptosis in plasma-treated cells at 5 and 15 J/cm2. Plasma treatment induces apoptosis in melanoma cells through a pathway that appears to be dependent on production of intracellular ROS. DBD plasma production of intracellular ROS leads to dose-dependent DNA damage in melanoma cells, detected by γ-H2AX, which was completely abrogated by pre-treating cells with ROS scavenger, NAC. Plasma-induced DNA damage in turn may lead to the observed plasma-induced apoptosis. Since plasma is non-thermal, it may be used to selectively treat malignancies.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Non-thermal Plasma Induces Apoptosis in Melanoma Cells via Production of Intracellular Reactive Oxygen Species

et al. 2010

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“…It has been demonstrated that proliferation of endothelial cells can be increased significantly through treatment by NTGP in air. Viable endothelial cell (PAEC) number was enhanced by a low dose floating electrode, dielectric barrier discharge (FE‐DBD) plasma treatment 105–107. These cells showed greater viable cell number than controls for up to 30 s of plasma treatment.…”

Section: How Can Ntgps Address These Unmet Needs?mentioning

confidence: 99%

Gas Plasma: Medical Uses and Developments in Wound Care

Lloyd

Friedman

Jafri

et al. 2010

Plasma Processes & Polymers

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Non‐thermal gas plasmas (NTGPs) are a promising emergent medical technology.a Unlike thermal plasmas, they generate a complex room temperature mix of reactive species which interact with tissues. The characterization of candidate plasmas and their interaction with tissues has shown that they produce a variety of broadly dose dependent effects ranging from fibroblast proliferation to angiogenesis and bacterial destruction. These findings, supported by recent experiments using skin models, suggest that NTGP's could potentially play an important role in both decontaminating acute and chronic wounds and accelerating healing. However, important issues over toxicological and environmental safety remain unanswered and a number of regulatory and technical hurdles will need to be overcome before a NTGP medical device is released.

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