On the interaction of alginate-based core-shell nanocarriers with keratinocytes in vitro

Nguyen, Hoang Truc Phuong; Allard-Vannier, Émilie; Gaillard, Cédric; Eddaoudi, Imane; Miloudi, Lynda; Soucé, Martin; Chourpa, Igor; Munnier, Emilie

doi:10.1016/j.colsurfb.2016.02.055

Cited by 14 publications

(12 citation statements)

References 48 publications

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“…Our findings are in well agreement with the recent study, where very similar structures, however without addition of Transcutol HP demonstrated also nonsignificant toxicity on human keratinocytes (HaCaT) cell line even at very high concentration [49]. In our test, we used Eb concentrations 0.01, 0.1 and 1 μg/ml, respectively because in this particular concentration range, inhibitory effects of Eb-NCS 3 on Candida spp.…”

Section: Discussionsupporting

confidence: 80%

Topical Delivery of Ebselen Encapsulated in Biopolymeric Nanocapsules: Drug Repurposing Enhanced Antifungal Activity

Jaromin

Żarnowski

Pįętka-Ottlik

et al. 2018

Nanomedicine (Lond.)

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Aim: Ebselen (Eb) is an example of a repurposed drug with poor aqueous solubility which requires sophisticated delivery system such as nanoencapsulation in nanocapsules for topical application. Materials & methods: Eb-nanocapsules were examined for morphology, activity against Candida spp., cytotoxicity and skin permeation. Results: Eb-nanocapsules were active against skin-infecting Candida tropicalis, Candida albicans and Candida parapsilosis yeasts (minimal inhibitory concentration values were about 4-, 2-and 1.25-times lower vs free Eb, respectively) and able to suppress induced lipid oxidation in the oil/water emulsion. Moreover, demonstrated minimal toxicity in normal human dermal fibroblast cell line, whereas ex vivo skin permeation studies showed no transdermal passage and strong interactions with stratum corneum. Conclusion: Eb-nanocapsules represent a promising, safe and complementary alternative to the treatment of cutaneous candidiasis.

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Section: Discussionsupporting

confidence: 80%

Topical Delivery of Ebselen Encapsulated in Biopolymeric Nanocapsules: Drug Repurposing Enhanced Antifungal Activity

Jaromin

Żarnowski

Pįętka-Ottlik

et al. 2018

Nanomedicine (Lond.)

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“…However, the molecular specificity of the analysis enables comparative studies between different formulations such as gels or creams, with similar sensitivities. Notably, nowadays different encapsulating protocols are being optimized with the aim of enhancing ACI and API penetration through the Stratum corneum in order to increase the concentrations in the epidermis . Developing efficient and specific multivariate methods will support the transfer of confocal Raman spectroscopic imaging as reference technique in industrial routines.…”

Section: Resultsmentioning

confidence: 99%

Confocal Raman spectroscopic imaging for in vitro monitoring of active ingredient penetration and distribution in reconstructed human epidermis model

Miloudi

Bonnier

Tfayli³

et al. 2017

Journal of Biophotonics

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Topically applied active cosmetic ingredients (ACI) or active pharmaceutical ingredients (API) efficacy is directly related to their efficiency of penetration in the skin. In vitro reconstructed human epidermis surrogate models offer in vivo like skin samples for transdermal studies. Using Delipidol®, an ACI currently used in the cosmetics industry, the capabilities to deliver accurate distribution maps and penetration profiles of this molecule by means of confocal Raman spectroscopic imaging have been demonstrated. Using a non-negative constrained least squares (NCLS) approach, contribution of specific molecules can be estimated at each point of spectral maps in order to deliver semi-quantitative heat maps representing the ACI levels in the different skin layers. The concentration profiles obtained are approximately single exponential for all 3 time points evaluated, with a consistent decay constant, which is independent of the sublayer structure. Notably, however, there is no significant penetration into the lower basal layers until a critical concentration is built up, after 3 hours. Combination of Raman confocal imaging with spectral unmixing methods such as NCLS is demonstrated to be a relevant approach for in vitro biological evaluation of cosmetic and pharmaceutical active ingredients and could easily be implemented as a screening tool for industrial use.

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“…After 24 h or 48h at 37 °C, non-adherent microorganisms were removed by aspiration. Wells were then washed with PBS and observed under classical inverted optical microscope (IX51® inverted microscope, Olympus) for visual inspection prior to biofilm quantification using a previously described metabolic assay based on the reduction of a tetrazolium salt (XTT) [26,27]. Briefly, 300 mg/L XTT and 0.13 mM menadione were added to 200 μL of PBS in each well.…”

Section: Anti-biofilm Growthmentioning

confidence: 99%

Spirulina platensis sustainable lipid extracts in alginate-based nanocarriers: An algal approach against biofilms

et al. 2019

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On the interaction of alginate-based core-shell nanocarriers with keratinocytes in vitro

Cited by 14 publications

References 48 publications

Topical Delivery of Ebselen Encapsulated in Biopolymeric Nanocapsules: Drug Repurposing Enhanced Antifungal Activity

Topical Delivery of Ebselen Encapsulated in Biopolymeric Nanocapsules: Drug Repurposing Enhanced Antifungal Activity

Confocal Raman spectroscopic imaging for in vitro monitoring of active ingredient penetration and distribution in reconstructed human epidermis model

Spirulina platensis sustainable lipid extracts in alginate-based nanocarriers: An algal approach against biofilms

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