On the assessment of angiogenesis: its time to change (go further) from an estimate to a measurement

Grizzi, Fabio; Chiriva‐Internati, Maurizio; Yiu, Daniel

doi:10.5603/fm.a2019.0096

Cited by 1 publication

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“…Anti-angiogenic therapy, has become one of the most important means in treating malignant tumors by blocking the supply of oxygen and nutrients of tumors (Folkman, 1971 ; Grizzi et al., 2020 ). In the present study, we found that endothelial cells could not only form vessels to supply oxygen and nutrients for HCC but could also envelop tumor cells to protect them from blood flow shear stress and immune attack ( Figure 6(A) ).…”

Section: Discussionmentioning

confidence: 99%

Targeting of the COX-2/PGE2 axis enhances the antitumor activity of T7 peptide in vitro and in vivo

et al. 2021

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T7 peptide is considered as an antiangiogenic polypeptide. The presents study aimed to further detect the antiangiogenic mechanisms of T7 peptide and determine whether combining T7 peptide and meloxicam (COX-2/PGE2 specific inhibitor) could offer a better therapy to combat hepatocellular carcinoma (HCC). T7 peptide suppressed the proliferation, migration, tube formation, and promoted the apoptosis of endothelial cells under both normoxic and hypoxic conditions via integrin α3β1 and αvβ3 pathways. Cell proliferation, migration, apoptosis, or tube formation ability were detected, and the expression of integrin-associated regulatory proteins was detected. The anti-tumor activity of T7 peptide, meloxicam, and their combination were evaluated in HCC tumor models established in mice. T7 peptide suppressed the proliferation, migration, tube formation, and promoted the apoptosis of endothelial cells under both normoxic and hypoxic conditions via integrin α3β1 and αvβ3 pathways. Meloxicam enhanced the activity of T7 peptide under hypoxic condition. T7 peptide partly inhibited COX-2 expression via integrin α3β1 not αvβ3-dependent pathways under hypoxic condition. T7 peptide regulated apoptosis associated protein through MAPK-dependent and -independent pathways under hypoxic condition. The MAPK pathway was activated by the COX-2/PGE2 axis under hypoxic condition. The combination of T7 and meloxicam showed a stronger anti-tumor effect against HCC tumors in mice. The data highlight that meloxicam enhanced the antiangiogenic activity of T7 peptide in vitro and in vivo .

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Section: Discussionmentioning

confidence: 99%