On-site applicable diagnostic fluorescent probe for fire blight bacteria

Jung, Yuna; Jin, Ji Hye; Jung, Minji; Hwang, In Sun; Vu, Nguyen Trung; Park, Yoon Kyung; Park, Ki Ho; Oh, Chang‐Sik; Kim, Dokyoung

doi:10.1016/j.isci.2023.106557

Cited by 3 publications

(3 citation statements)

References 52 publications

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“…For the information of NMR and Mass spectroscopy of each compound, please refer to Jung et al. 1 Synthesis of Compound 1. Flame dry the 10 mL round bottom flask with the stirring bar.…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“…We recently reported the fluorescent probe B-1 that can visualize E. amylovora in plants or objects infected with fire blight. 1 B-1 emits negligible fluorescence with an excitation wavelength of 551 nm. However, the fluorescence intensity of B-1, especially at 686 nm, increases remarkably and emits red fluorescence under the presence of E. amylovora .…”

Section: Before You Beginmentioning

confidence: 99%

“…0.1 in OD 600 and 0.55 in Mcfarland turbidity refers to 2 × 10 8 colony-forming unit (CFU)/mL. 1 Optional: bacterial stock preparation …”

Section: Before You Beginmentioning

confidence: 99%

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Protocol for diagnosing Erwinia amylovora infection using a fluorescent probe

Jin

Kim

2023

STAR Protocols

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“…For the information of NMR and Mass spectroscopy of each compound, please refer to Jung et al. 1 Synthesis of Compound 1. Flame dry the 10 mL round bottom flask with the stirring bar.…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

Section: Before You Beginmentioning

confidence: 99%

See 1 more Smart Citation

Protocol for diagnosing Erwinia amylovora infection using a fluorescent probe

Jin

Kim

2023

STAR Protocols

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Multicolorimetric Sensor Array Based on Silver Metallization of Gold Nanorods for Discriminating Dopaminergic Agents

Koohsar,

Orouji,

Hormozi-Nezhad

2024

ACS Chem. Neurosci.

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Single-Cell Detection of Erwinia amylovora Using Bio-Functionalized SIS Sensor

Lee,

Oh,

Kwon

et al. 2023

Sensors

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Herein, we developed a bio-functionalized solution-immersed silicon (SIS) sensor at the single-cell level to identify Erwinia amylovora (E. amylovora), a highly infectious bacterial pathogen responsible for fire blight, which is notorious for its rapid spread and destructive impact on apple and pear orchards. This method allows for ultra-sensitive measurements without pre-amplification or labeling compared to conventional methods. To detect a single cell of E. amylovora, we used Lipopolysaccharide Transporter E (LptE), which is involved in the assembly of lipopolysaccharide (LPS) at the surface of the outer membrane of E. amylovora, as a capture agent. We confirmed that LptE interacts with E. amylovora via LPS through in-house ELISA analysis, then used it to construct the sensor chip by immobilizing the capture molecule on the sensor surface modified with 3′-Aminopropyl triethoxysilane (APTES) and glutaraldehyde (GA). The LptE-based SIS sensor exhibited the sensitive and specific detection of the target bacterial cell in real time. The dose–response curve shows a linearity (R2 > 0.992) with wide dynamic ranges from 1 to 107 cells/mL for the target bacterial pathogen. The sensor showed the value change (dΨ) of approximately 0.008° for growing overlayer thickness induced from a single-cell E. amylovora, while no change in the control bacterial cell (Bacillus subtilis) was observed, or negligible change, if any. Furthermore, the bacterial sensor demonstrated a potential for the continuous detection of E. amylovora through simple surface regeneration, enabling its reusability. Taken together, our system has the potential to be applied in fields where early symptoms are not observed and where single-cell or ultra-sensitive detection is required, such as plant bacterial pathogen detection, foodborne pathogen monitoring and analysis, and pathogenic microbial diagnosis.

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On-site applicable diagnostic fluorescent probe for fire blight bacteria

Cited by 3 publications

References 52 publications

Protocol for diagnosing Erwinia amylovora infection using a fluorescent probe

Protocol for diagnosing Erwinia amylovora infection using a fluorescent probe

Multicolorimetric Sensor Array Based on Silver Metallization of Gold Nanorods for Discriminating Dopaminergic Agents

Single-Cell Detection of Erwinia amylovora Using Bio-Functionalized SIS Sensor

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