On-ratio PDMS bonding for multilayer microfluidic device fabrication

Lai, Andre; Altemose, Nicolas; White, Jonathan A.; Streets, Aaron

doi:10.1101/450544

Cited by 3 publications

(2 citation statements)

References 25 publications

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“…Devices were fabricated by multilayer soft lithography ( Unger et al, 2000 ). On-ratio 10:1 base:crosslinker RTV615A PDMS (Momentive Performance Materials, Inc., Waterford, NY) was used for both layers, and layer bonding was performed by partial curing, followed by alignment, then full curing ( Lai et al, 2019 ). To prevent PDMS adhesion to the molds, the molds were silanized by exposure to trichloromethlysilane (Sigma-Aldrich, St. Louis, MO) vapor under vacuum for 20 min.…”

Section: Methodsmentioning

confidence: 99%

μDamID: A Microfluidic Approach for Joint Imaging and Sequencing of Protein-DNA Interactions in Single Cells

et al. 2020

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Section: Methodsmentioning

confidence: 99%

μDamID: A Microfluidic Approach for Joint Imaging and Sequencing of Protein-DNA Interactions in Single Cells

et al. 2020

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“…The process of fabricating the microlens grating array is shown in Figure 3, and there are four main steps: PDMS material preparation process, primary replication process, anti-adhesive treatment process, and secondary replication process. The PDMS material preparation is the basis for all other processes, different pre-polymer to curing agent ratios significantly affect the refractive index, reflectivity and dispersion of PDMS polymers 40 ; Primary replication process is to fabricate the microlens array elastic mode; Anti-adhesive treatment process is to coat the microlens array elastic mode with antiadhesive coating, using molecular vapor deposition (MVD)；Secondary replication is done by using the microlens array elastic inversion and diffraction grating with anti-adhesion treatment as a template for the finished microlens grating array.…”

Section: Fabricationmentioning

confidence: 99%

Fabrication of a micro lens array-grating for a miniature spectrometer

Shan¹,

Liu²,

Li³

et al. 2022

Optical Design and Testing XII

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For the demand of line chromatic confocal displacement measurement, a new multi-channel compact spectrometer needs to be developed. In this paper, a microlens array-grating structure, in which one side is microlens array and the other side is blazed gating, is proposed for a miniature spectrometer. The microlens array-grating structure can be pressed in the form of "sandwich" by soft lithography using PDMS material. The micro lens array is a 10*10 mm square, consisting of 45*45 microlens elements, each with a diameter of 220 μm, and the outer diameters of the elements are closely connected with each other. The blazed grating is an equal period grating with a grating line density of 600 lines /mm and a blazed angle of 8°37’. By optimizing the design parameters, the image resolution of the spectrometer based on the grating-microlens array is within 1 nm in the wide band of 400 nm~700 nm. Different microlens grating surface elements have performance consistency with a standard deviation of about 0.5 nm, which can realize the function of arrayed micro-spectrometer.

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μDamID: a microfluidic approach for imaging and sequencing protein-DNA interactions in single cells

Altemose¹,

Maslan²,

Lai³

et al. 2019

Preprint

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1 2 Genome regulation depends on carefully programmed protein-DNA interactions that maintain or 3 alter gene expression states, often by influencing chromatin organization. Most studies of these 4 interactions to date have relied on bulk methods, which in many systems cannot capture the 5 dynamic single-cell nature of these interactions as they modulate cell states. One method allowing 6 for sensitive single-cell mapping of protein-DNA interactions is DNA adenine methyltransferase 7 identification (DamID), which records a protein's DNA-binding history by methylating adenine 8 bases in its vicinity, then selectively amplifies and sequences these methylated regions. These 9interaction sites can also be visualized using fluorescent proteins that bind to methyladenines. Here 10 we combine these imaging and sequencing technologies in an integrated microfluidic platform 11 (µDamID) that enables single-cell isolation, imaging, and sorting, followed by DamID. We apply 12 this system to generate paired single-cell imaging and sequencing data from a human cell line, in 13 which we map and validate interactions between DNA and nuclear lamina proteins, providing a 14 measure of 3D chromatin organization and broad gene regulation patterns. µDamID provides the 15 unique ability to compare paired imaging and sequencing data for each cell and between cells, 16 enabling the joint analysis of the nuclear localization, sequence identity, and variability of protein-17

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On-ratio PDMS bonding for multilayer microfluidic device fabrication

Cited by 3 publications

References 25 publications

μDamID: A Microfluidic Approach for Joint Imaging and Sequencing of Protein-DNA Interactions in Single Cells

μDamID: A Microfluidic Approach for Joint Imaging and Sequencing of Protein-DNA Interactions in Single Cells

Fabrication of a micro lens array-grating for a miniature spectrometer

μDamID: a microfluidic approach for imaging and sequencing protein-DNA interactions in single cells

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