On-off-on fluorescent sensor for glutathione based on bifunctional vanadium oxide quantum dots induced spontaneous formation of MnO2 nanosheets

Jia, Pei; Hou, Jinjie; Yang, Kairong; Wang, Li

doi:10.1007/s00604-021-04958-z

Cited by 14 publications

(7 citation statements)

References 33 publications

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“…Moreover, applications where sub-micromolar GSH concentrations need to be determined can be covered by this chlorin-based fluorescent probe, with a LOD

for TCPC:Hg 2+ 1:2 as low as 40 nM. This value, susceptible to further reduction by reducing the TCPC:Hg 2+ ratio, is significantly lower than those found in most of the existing literature using fluorescent probes [ 32 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 ] and very similar to those reporting the lowest values [ 53 , 67 , 75 ]. These results are summarized in Table S1 .…”

Section: Resultsmentioning

confidence: 99%

Selective Determination of Glutathione Using a Highly Emissive Fluorescent Probe Based on a Pyrrolidine-Fused Chlorin

et al. 2023

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We report the use of a carboxylated pyrrolidine-fused chlorin (TCPC) as a fluorescent probe for the determination of glutathione (GSH) in 7.4 pH phosphate buffer. TCPC is a very stable, highly emissive molecule that has been easily obtained from meso-tetrakis(4-methoxycarbonylphenyl) porphyrin (TCPP) through a 1,3-dipolar cycloaddition approach. First, we describe the coordination of TCPC with Hg(II) ions and the corresponding spectral changes, mainly characterized by a strong quenching of the chlorin emission band. Then, the TCPC-Hg2+ complex exhibits a significant fluorescence turn-on in the presence of low concentrations of the target analyte GSH. The efficacy of the sensing molecule was tested by using different TCPC:Hg2+ concentration ratios (1:2, 1:5 and 1:10) that gave rise to sigmoidal response curves in all cases with modulating detection limits, being the lowest 40 nM. The experiments were carried out under physiological conditions and the selectivity of the system was demonstrated against a number of potential interferents, including cysteine. Furthermore, the TCPC macrocycle did not showed a significant fluorescent quenching in the presence of other metal ions.

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“…Moreover, applications where sub-micromolar GSH concentrations need to be determined can be covered by this chlorin-based fluorescent probe, with a LOD

Section: Resultsmentioning

confidence: 99%

Selective Determination of Glutathione Using a Highly Emissive Fluorescent Probe Based on a Pyrrolidine-Fused Chlorin

et al. 2023

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“…33 The peak at 989 cm −1 was the V�O stretching band, 34 while 885 and 520 cm −1 , respectively, corresponded to the vibration modes of V−O−V and VO 2 . 35 The microscopic morphological characteristics of the VNSs were obtained by atomic force microscopy with a surface roughness of 10.7 nm (Figure S4A). A thermogravimetric study demonstrates that VNSs have acceptable thermal stability as well (Figure S4B).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Double-Enzyme Active Vanadium Nanospheres-Mediated Ratiometric Multicolor Immunosensors for Sensitive Detection of the T-2 Toxin

Cao

et al. 2023

Anal. Chem.

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Owing to its high throughput, simplicity, and rapidity, enzyme-linked immunosorbent assay (ELISA) has attracted much attention in the field of immunoassays. However, the traditional ELISA usually affords a single signal readout and the labeling ability of the enzyme used is poor, resulting in low accuracy and a limited detection range. Herein, a vanadium nanospheres (VNSs)-mediated competitive ratio nanozymes-linked immunosorbent assay (VNSs-RNLISA) was created for the sensitive detection of the T-2 toxin (T-2). As the key to the biosensor, the VNSs with superoxide dismutase-like and peroxidase-like dual-enzyme mimetic activities were synthesized by a one-step hydrothermal method, which oxidized 1,1-diphenyl-2-picryl-hydrazyl fading and catalyzed 3,3′,5,5′-tetramethylbenzidine (TMB) color development. Therefore, T-2 could not only be qualitatively measured with the naked eye but also be quantitatively evaluated by monitoring the ratio of absorbance at 450 and 517 nm wavelengths. Moreover, the characterization of a VNSs-labeled antibody probe showed strong dual-enzymatic activity, excellent stability, and high affinity with T-2 [the affinity constant (k a) was approximately 1.36 × 108 M–1], which can significantly improve the detection sensitivity. The limit of detection of VNSs-RNLISA was 0.021 ng/mL, which was approximately 27-fold more sensitive than the single signal nanozymes-linked immunosorbent assay (0.561 ng/mL). Besides, the change in the ratio of absorbance (Δ450/Δ517) decreased linearly in a range of 0.22–13.17 ng/mL, outperforming the detection range of a single-mode nano-enzyme-linked immunosorbent assay using TMB by a factor of 1.6 times. Furthermore, the VNSs-RNLISA was successfully used to identify T-2 in maize and oat samples, with recoveries ranging from 84.216 to 125.371%. Overall, this tactic offered a promising platform for the quick detection of T-2 in food and might broaden the application range of the enzyme-linked immunosorbent assay.

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“…This “switch-on” platform has a response in the range of 5–50 μM and recorded a high LOD of 2.96 μM (Figure e). Recently, Jia et al synthesized bifunctional vanadium oxide quantum dots (VO x QDs) with strong fluorescence and redox ability via cryogenic-mediated liquid-phase exfoliation and then employed them to construct an “on–off–on” fluorescence sensor for GSH . VO x QDs exhibited blue emission at 410 nm and also played the role of reductant to spontaneously induce the formation of MnO 2 NSs from KMnO 4 .…”

Section: Sensing Applicationsmentioning

confidence: 99%

“…Recently, Jia et al synthesized bifunctional vanadium oxide quantum dots (VO x QDs) with strong fluorescence and redox ability via cryogenic-mediated liquid-phase exfoliation and then employed them to construct an "on−off−on" fluorescence sensor for GSH. 195 VO x QDs exhibited blue emission at 410 nm and also played the role of reductant to spontaneously induce the formation of MnO 2 NSs from KMnO 4 . MnO 2 NSs could quench the fluorescence of VO x QDs, and the quenching process was reversed in the presence of GSH (Figure 38f).…”

Section: Sensing Applicationsmentioning

confidence: 99%

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Review of 2D MnO₂ Nanosheets as FRET-Based Nanodot Fluorescence Quenchers in Chemosensing Applications

Hiremath

Banerjee

Chatterjee

2022

ACS Appl. Nano Mater.

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In the past decade, a variety of MnO 2 nanostructures have been used in chemosensing applications because of their unique redox properties, structural diversity, and broad absorption band, which makes them good energy acceptors. The most common strategy in Forster (or fluorescence) resonance energy transfer (FRET)-based chemosensing assays is to use low-dimensional fluorescent dots like carbon-based dots, metal dots, and even fluorescent dyes to form donor−acceptor pairs with 2D MnO 2 nanosheets (NSs). This review is a collection of the current library on fluorescent nanodots and MnO 2 NSs-based FRET conjugates as sensing platforms with a focus on highlighting the strategies involved in the detection of various analytes. Commonly, an analyte-triggered redox reaction results in the decomposition of MnO 2 NSs, leading to the release of fluorescent nanodots in solution for the turn-on detection of various reducing species. We intend to elaborately discuss the systematic upgradation of detection strategies involving fluorescent dots@2D MnO 2 NSs. Recently, a cascade of redox reactions was judiciously employed to target several bioanalytes involving a fluorescent dots@MnO 2 NSs FRET conjugate in a selective and sensitive manner. These sensing assays are categorized by the use of different fluorescent nanodots [carbon dots (CDs) and graphene quantum dots (GQDs), polymer dots, metal dots, etc.] with 2D MnO 2 NSs as FRET pairs in different sensing applications. Further, the analytes were categorized as reducing species, oxidants, enzymes, microbes, etc. The focus was paid to the sensing efficiency of the analytical tool. As a part of the study, a brief account of the synthesis of nanodots and MnO 2 NSs, their properties, and the FRET mechanism is also included. In a few cases, the sensing mechanism follows an inner filter effect without a prerequisite of the distance between the fluorophore and quencher units in the sensing of analytes, which are also covered in this collection. The available literature indicates a bright future for these FRET-based contemporary sensing strategies as environmentally benign, cost-effective, label-free techniques, which are discussed in the end.

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On-off-on fluorescent sensor for glutathione based on bifunctional vanadium oxide quantum dots induced spontaneous formation of MnO2 nanosheets

Cited by 14 publications

References 33 publications

Selective Determination of Glutathione Using a Highly Emissive Fluorescent Probe Based on a Pyrrolidine-Fused Chlorin

Selective Determination of Glutathione Using a Highly Emissive Fluorescent Probe Based on a Pyrrolidine-Fused Chlorin

Double-Enzyme Active Vanadium Nanospheres-Mediated Ratiometric Multicolor Immunosensors for Sensitive Detection of the T-2 Toxin

Review of 2D MnO₂ Nanosheets as FRET-Based Nanodot Fluorescence Quenchers in Chemosensing Applications

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On-off-on fluorescent sensor for glutathione based on bifunctional vanadium oxide quantum dots induced spontaneous formation of MnO2 nanosheets

Cited by 14 publications

References 33 publications

Selective Determination of Glutathione Using a Highly Emissive Fluorescent Probe Based on a Pyrrolidine-Fused Chlorin

Selective Determination of Glutathione Using a Highly Emissive Fluorescent Probe Based on a Pyrrolidine-Fused Chlorin

Double-Enzyme Active Vanadium Nanospheres-Mediated Ratiometric Multicolor Immunosensors for Sensitive Detection of the T-2 Toxin

Review of 2D MnO2 Nanosheets as FRET-Based Nanodot Fluorescence Quenchers in Chemosensing Applications

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Review of 2D MnO₂ Nanosheets as FRET-Based Nanodot Fluorescence Quenchers in Chemosensing Applications