Olive-mill wastewaters: a promising substrate for microbial lipase production

D’Annibale, Alessandro; Sermanni, Giovanni Giovannozzi; Federici, Federico; Petruccioli, Maurizio

doi:10.1016/j.biortech.2005.09.001

Cited by 145 publications

(105 citation statements)

References 35 publications

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“…The chemical composition of 3POMWW is complex due to the water from the milled olives (vegetation water) and the soft tissues from the olive fruit. Typical composition of three-phase olive mill wastewaters is: pH 5.04, COD 43.0 g L -1 (COD: Chemical Oxigen Demand), total sugars 17.4 g L -1 , total phenols 2.5 g L -1 and lipids 0.75 g L -1 (D'Annibale et al, 2006).…”

Section: Waste Quantities and Characteristicsmentioning

confidence: 99%

“…The 3POMWW have also been studied as a source of natural pigments (anthocyanins) and different exopolysaccharides, and as a growth medium for algae (Ramos-Cormenzana et al, 1995). 3POMWW have been used as a growth media for the microbial production of extra-cellular lipase (D'Annibale et al, 2006) and for composting with olive leaves (Michailides et al, 2011).…”

Section: Three-phase Olive Mill Wastewater (3pomww)mentioning

confidence: 99%

See 1 more Smart Citation

Olive Oil Mill Waste Treatment:Improving the Sustainability of the Olive Oil Industry with Anaerobic Digestion Technology

Rincón¹,

Fermoso²,

Borja³

2012

Olive Oil - Constituents, Quality, Health Properties and Bioconversions

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Section: Waste Quantities and Characteristicsmentioning

confidence: 99%

Section: Three-phase Olive Mill Wastewater (3pomww)mentioning

confidence: 99%

Olive Oil Mill Waste Treatment:Improving the Sustainability of the Olive Oil Industry with Anaerobic Digestion Technology

Rincón¹,

Fermoso²,

Borja³

2012

Olive Oil - Constituents, Quality, Health Properties and Bioconversions

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“…Few studies reported that oil cakes as the best solid substrate-cum-inducer for the production of lipase by SSF [14] [15]. D'Annibale et al [16] reported that olive mill waste water as a growth medium for lipase production, which showed the highest lipase activity of 9.23 U/ml. Brozzoli et al [17] studied the lipase production in bench-top reactor using the olive mill waste water medium and obtained the maximum production as 20.4 U/ml.…”

Section: Discussionmentioning

confidence: 99%

Optimization of Parameters for the Production of Lipase from Pseudomonas sp. BUP6 by Solid State Fermentation

Faisal¹,

Hareesh²,

Priji³

et al. 2014

AER

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Solid-state fermentation (SSF) holds tremendous potentials for the production of industrially significant enzymes. The present study describes the production of lipase by a novel rumen bacterium, Pseudomonas sp. strain BUP6 on agro-industrial residues. Pseudomonas sp. strain BUP6 showed higher lipase production when grown in Basal salt medium (BSM) supplemented with oil cakes. Initially, five different oil cakes (obtained after extracting oil from coconut, groundnut, cotton seed, gingelly or soybean) were screened to find out the most suitable substrate-cum-inducer for the production of lipase. Among them, groundnut cake supported the maximum production of lipase (107.44 U/gds). Box-Behnken Design (BBD), followed by response surface methodology (RSM) was employed to optimize the culture parameters for maximizing the production of lipase. Using the software Minitab 14, four different parameters like temperature, pH, moisture content and incubation time were selected for the statistical optimization, which resulted in 0.7 fold increase (i.e., 180.75 U/gds) in production of lipase under the optimum culture conditions (temperature 28˚C, pH 5.9, moisture 33% and incubation 2 d). Thus, this study signifies the importance of SSF for the production of industrially-significant lipase using agro-industrial residues as solid support.* Corresponding author. P. A. Faisal et al.126

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“…From the industrial point of view these are considered very important, due to their great production potential on a large scale and to the capacity of deterioration of microorganisms (KIRK et al, 2002). Fungal lipases are among the most important commercial enzymes and the Rhizopus species are well-known lipolytic microorganisms (D ANNIBALE et al, 2005;YANG et al, 2005). This work reports on the isolation, characterization, and some biochemical aspects of the lipase secreted by Rhizopus sp.…”

Section: Introductionmentioning

confidence: 99%

Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.

Koblitz

Pastore

2006

Ciênc. agrotec.

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The present study had as a goal to purify and characterize the lipolytic fraction secreted by a strain of Rhizopus sp. Only 3 steps of purification were necessary to achieve SDS-PAGE homogeneity. One band with 37.5 KDa molecular mass and with 1446 U/ mg specific activity was obtained. The purified fraction presented 2 lipase isoforms; both showed optimum activity at 50ºC, and were stable between 6.5 and 7.5 pH values and at temperatures below 50ºC and also kept their activity in hexane. The lipase was inactivated by Hg +2 and by n-bromosuccinimide and activated by Na + .Index terms: Lipase, Rhizopus sp., purification, biochemical characterization. RESUMOO presente trabalho teve por objetivo purificar e caracterizar a fração lipolítica secretada por uma linhagem de Rhizopus sp. Apenas 3 etapas de purificação foram necessárias para alcançar homogeneidade em eletroforese de proteína desnaturada. Uma única banda com massa molecular de 37,5 KDa e atividade específica de 1446U/mg foi obtida. A fração purificada apresentou 2 isoformas de lipase, ambas com temperatura ótima de atividade igual a 50ºC, mantiveram-se estáveis entre valores de pH entre 6,5 e 7,5 e a temperaturas inferiores a 50ºC e mantiveram sua atividade na presença de hexano. A fração lipolítica foi inativada por Hg + e por nbromosuccinimida e ativada por Na + .Termos para indexação: Lipase, Rhizopus sp., purificação, caracterização bioquímica.

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Olive-mill wastewaters: a promising substrate for microbial lipase production

Cited by 145 publications

References 35 publications

Olive Oil Mill Waste Treatment:Improving the Sustainability of the Olive Oil Industry with Anaerobic Digestion Technology

Olive Oil Mill Waste Treatment:Improving the Sustainability of the Olive Oil Industry with Anaerobic Digestion Technology

Optimization of Parameters for the Production of Lipase from Pseudomonas sp. BUP6 by Solid State Fermentation

Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.

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