Olfactory Signal Transduction in the Mouse Septal Organ

Ma, Minghong; Grosmaître, Xavier; Iwema, Carrie; Baker, Harriet; Greer, Charles A.; Shepherd, Gordon M.

doi:10.1523/jneurosci.23-01-00317.2003

Cited by 96 publications

(59 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1 A). Because its size is ϳ1% of the MOE, and most neurons in this area express G olf (Ma et al, 2003), we hypothesized that the septal organ expresses a subset of the G-protein-coupled olfactory receptor genes from the same superfamily as the MOE. We first took a standard cDNA cloning approach to identify candidate receptor genes using degenerate primers based on the conserved regions of the mouse olfactory receptors (Zhang and Firestein, 2002).…”

Section: Resultsmentioning

confidence: 99%

“…Two male and two female groups of C57BL/6 mice (8 -16 weeks, five animals in each group) were deeply anesthetized and decapitated. The septal organ was harvested under a microscope, where it was readily separated from the MOE to avoid any contamination from the latter (Ma et al, 2003), and immersed in RNAlater solution immediately. Total RNAs were isolated using the RNeasy Mini kit with an oncolumn DNase digestion step to eliminate genomic DNA contamination.…”

Section: Methodsmentioning

confidence: 99%

“…The septal organ is a small patch of olfactory neuroepithelium at the ventral base of the nasal septum found in many mammals (Rodolfo-Masera, 1943;Adams and McFarland, 1971;Kratzing, 1984). Because most of the sensory neurons in this area express olfactory-specific G-protein (G olf ) (Ma et al, 2003), we hypothesized that the septal organ, similar to the MOE, expresses G-protein-coupled olfactory receptors from the same superfamily (Kaluza et al, 2004). A major goal of this study was to identify a relatively complete list of receptor genes (from a pool of ϳ1400) in the septal organ.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular Organization of the Olfactory Septal Organ

Tian¹,

Ma²

2004

J. Neurosci.

View full text Add to dashboard Cite

The septal organ in the mammalian nose is a distinct chemosensory organ sitting in the air path. To gain insights into its organization and function, we analyzed the chemoreceptors expressed in this area. By combining cDNA cloning, Affymetrix (Santa Clara, CA) genechips covering all the mouse olfactory receptor genes, and in situ hybridization, we achieved a relatively complete expression profile of the olfactory receptor genes in the septal organ. The majority of the septal neurons express only a few receptor genes in varying patterns, with the top one in ϳ50% of the cells and the top eight together in ϳ93% of the cells. We demonstrated that a single neuron expresses only one receptor by a thorough combination of all the major septal receptor genes in double-labeling studies. These septal receptor genes do not form a single subfamily. Instead, these genes are distributed on a few major branches of the phylogenetic tree covering all the mouse olfactory receptors. Most of these genes are also concentrated in certain areas within the most ventral zone of the main olfactory epithelium, although their expression patterns do not match those in the septal organ. In contrary to the previous view of random distribution, our results indicate that certain olfactory receptors form "hot spots" in the nose.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Organization of the Olfactory Septal Organ

Tian¹,

Ma²

2004

J. Neurosci.

View full text Add to dashboard Cite

show abstract

“…The SO axons target the main OB (Ma et al, 2003;Tian and Ma, 2004) while the sensory neurons of the VNO target the AOB. Zone 4 and the SO project axons to similar locations (Astic and Saucier, 1988;Astic et al, 1987;Astic and Saucier, 1986;Giannetti et al, 1992;Saucier and Astic, 1986) and appear developmentally related (Astic and Saucier, 1988;Giannetti et al, 1995).…”

Section: Zonal Expression Of α-Internexinmentioning

confidence: 99%

Cytoskeletal organization of the developing mouse olfactory nerve layer

Akins

Greer

2005

J of Comparative Neurology

Self Cite

View full text Add to dashboard Cite

Olfactory sensory neuron (OSN) axonal extension and targeting occurs within the olfactory nerve layer (ONL) of the olfactory bulb (OB). The ONL can be differentiated into sublaminae: the outer (ONLo), where axons broadly target regions of the OB in tight fascicles, and inner (ONLi), where axons perform final targeting in loosely organized fascicles. During perinatal development cadherin-2 and its binding partner, gamma-catenin, are preferentially expressed by OSN axons in the ONLo versus the ONLi. Given the expression of these cytoskeletal associated molecules, we hypothesized that cytoskeletal elements of OSN axons may be differentially expressed across the ONL. We therefore examined cytoskeletal organization of OSN axons in the ONL, focusing on the day of birth (P0). We show that microfilaments, microtubules, and the intermediate filament (IF) vimentin are homogeneously expressed across the ONL at P0. In contrast, the IFs peripherin and alpha-internexin are preferentially localized to the ONLo at P0, with alpha-internexin expressed by a restricted subset of OSNs. We also show that OSN axons in the ONLo are significantly smaller than those in the ONLi. The data demonstrate that as OSN axons begin to exit the ONLo and target a specific region of the OB there is a down-regulation of cytoskeletal elements and bound extracellular adhesion molecules. The increase in axon diameter may reflect additional mechanisms involved in glomerular targeting or the formation of the large terminal boutons of OSN axons within glomeruli.

show abstract

“…The sense of smell plays an essential role for finding and evaluating food, avoiding predators and locating suitable mating partners. In most mammals, the olfactory system consists of at least four anatomically and functionally distinct peripheral subsystems: the main olfactory epithelium 1,2 , the Grueneberg ganglion 3,4 , the septal organ of Masera 5,6 and the vomeronasal organ. The VNO comprises the peripheral sensory structure of the accessory olfactory system (AOS), which plays a major role in detecting chemical cues that convey information about identity, gender, social rank and sexual state [7][8][9][10] .…”

Section: Introductionmentioning

confidence: 99%

In-depth Physiological Analysis of Defined Cell Populations in Acute Tissue Slices of the Mouse Vomeronasal Organ

Ackels

Drose

Spehr

2016

JoVE

View full text Add to dashboard Cite

In most mammals, the vomeronasal organ (VNO) is a chemosensory structure that detects both hetero-and conspecific social cues. Vomeronasal sensory neurons (VSNs) express a specific type of G protein-coupled receptor (GPCR) from at least three different chemoreceptor gene families allowing sensitive and specific detection of chemosensory cues. These families comprise the V1r and V2r gene families as well as the formyl peptide receptor (FPR)-related sequence (Fpr-rs) family of putative chemoreceptor genes. In order to understand the physiology of vomeronasal receptor-ligand interactions and downstream signaling, it is essential to identify the biophysical properties inherent to each specific class of VSNs.The physiological approach described here allows identification and in-depth analysis of a defined population of sensory neurons using a transgenic mouse line (Fpr-rs3-i-Venus). The use of this protocol, however, is not restricted to this specific line and thus can easily be extended to other genetically modified lines or wild type animals.

show abstract

Olfactory Signal Transduction in the Mouse Septal Organ

Cited by 96 publications

References 59 publications

Molecular Organization of the Olfactory Septal Organ

Molecular Organization of the Olfactory Septal Organ

Cytoskeletal organization of the developing mouse olfactory nerve layer

In-depth Physiological Analysis of Defined Cell Populations in Acute Tissue Slices of the Mouse Vomeronasal Organ

Contact Info

Product

Resources

About