Oleic acid inhibits stearic acid-induced inhibition of cell growth and pro-inflammatory responses in human aortic endothelial cells

Harvey, Kevin; Walker, Candace L.; Xu, Zhidong; Whitley, Phillip; Pavlina, Thomas M.; Hise, Mary; Zaloga, Gary P.; Siddiqui, Rafat A.

doi:10.1194/jlr.m010371

Cited by 99 publications

(90 citation statements)

References 47 publications

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“…Another study reported an increased expression of inflammatory genes, TNF-a after butter compared to olive oil and vegetable/fish oil [14]. A recent in vitro study demonstrated that presence of as little as 1/10 ratio of oleic acid to that of stearic acid attenuates stearic acid-included pro-inflammatory responses in endothelial cells by reducing cellular stearic acid incorporation and NF-jB activation [24]. It is therefore, possible that a lack of differences between cocoa butter and refined olive oil on serum TG, IL-6 and hsCRP is mainly due to attenuation of stearic a There was no difference in the effect of the test meals (Mixed model ANCOVA), but a significant increase was found over time for both test meals (P = 0.003), but there were difference between the two test meals (P = 0.41).…”

Section: Discussionmentioning

confidence: 97%

Dietary Cocoa Butter or Refined Olive Oil Does Not Alter Postprandial hsCRP and IL‐6 Concentrations in Healthy Women

Tholstrup

Teng

Raff

2011

Lipids

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Contrary to other long chain saturated fatty acids (SFA), fats high in stearic acid do not raise plasma cholesterol concentrations, however, a slight elevation in inflammatory markers, plasma fibrinogen and interleukin-6 (IL-6), has been observed in the fasting state. The effect of stearic acid on inflammation in the postprandial state has not yet been reported. We conducted a single blind crossover, randomized, postprandial study to compare the effects of a fat load of cocoa butter high in stearic acid and olive oil in ten healthy women. The test meals contained 1 g of fat per kg body weight (mean 62 g). Blood samples were collected at 0 (fasting), 4 and 6 h. Both diets resulted in a significant increase in serum triacylglycerol (TAG) concentration over time (P = 0.003) and a decrease in serum IL-6 concentration after 4 h followed by an increase to post absorptive values after 6 h (P < 0.001); whereas serum high sensitivity C-reactive protein (hsCRP) concentration was not affected. There was no difference between diets in effects on serum TAG, hsCRP and IL-6 concentrations and no association between postprandial lipemia and inflammatory markers. High intake of dietary fats increase postprandial serum TAG, however, may not affect inflammatory markers postprandially. Thus, fat rich in stearic acid does not seem to increase postprandial inflammation.

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Section: Discussionmentioning

confidence: 97%

Dietary Cocoa Butter or Refined Olive Oil Does Not Alter Postprandial hsCRP and IL‐6 Concentrations in Healthy Women

Tholstrup

Teng

Raff

2011

Lipids

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“…Moreover, in vitro experiments with human aortic endothelial cells have demonstrated that oleic acid neutralizes the negative impact of stearic acid on inflammatory signaling cascade by displacing stearic acid from phospholipid bilayer and then reducing NF-κB phosphorylation and ICAM-1 expression (Harvey et al, 2010b). Oleic acid also reduces VCAM-1 expression induced by TNF-α in endothelial cells through a mechanism involving both NF-κB activation and preservation of antioxidant systems (Massaro et al, 2002).…”

Section: Discussionmentioning

confidence: 97%

Phenolic content of extra virgin olive oil is essential to restore endothelial dysfunction but not to prevent vascular inflammation in atherosclerotic lesions of Apo E deficient mice

Claro

Ogalla

Rodríguez‐Rodríguez

et al. 2015

Journal of Functional Foods

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“…Previous studies indicated that C18:0 was the substrate for the synthesis of AA and C18:1. However, AA could not be synthesized with C18:1 and stearoyl-CoA desaturase could increase the content of C18:1, thereby inhibiting the AA reaction and achieving the purpose of relieving inflammation [17]. Moreover, C18:1 could relieve cell growth inhibition and pro-inflammatory effect caused by C18:0 and inhibit apoptosis and inflammation [18].…”

Section: Discussionmentioning

confidence: 98%

Dynamic analysis of phospholipid metabolism of mouse macrophages treated with common non-steroidal anti-inflammatory drugs

Peng

Zhao

et al. 2015

Mol Cell Biochem

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Through studying the changes of the total phospholipid components in mouse macrophages under the inflammatory status and the drug intervention status, we found the targets of non-steroidal anti-inflammatory drugs on phospholipids, thus providing the basis for the targets of in vitro anti-inflammatory effects of non-steroidal anti-inflammatory drugs. After RAW264.7 cells were pretreated with common non-steroidal anti-inflammatory drugs (aspirin and ibuprofen) and, respectively, stimulated with KLA for various periods (0.5, 4, 12, 16, and 24 h), the phospholipids were extracted. The dynamic changes of phospholipids in cells under various stimulations were analyzed with UPLC-Q-TOF-MS technique. Through the statistical analysis of Simca-P, we explored the potential targets of non-steroidal anti-inflammatory drugs on phospholipids. Through the dynamic analysis of phospholipids, we found two biomarkers (PC(17:1/18:1), PA(18:0/18:4)) which might be in vitro intervention inflammatory response targets of non-steroidal anti-inflammatory drugs. The analysis results show that in anti-inflammatory effects, non-steroidal anti-inflammatory drugs can inhibit COX, induce the cellular fatty acid desaturation and the changes of phospholipid components, stimulate free fatty acids, activate calcium ion channels of endoplasmic reticulum, and promote cell endocytosis, thus controlling inflammation and activating cells. Non-steroidal anti-inflammatory drugs can promote endocytosis, alter cell inflammatory response, and activate the process cells, thus realizing the anti-inflammatory effects.

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Oleic acid inhibits stearic acid-induced inhibition of cell growth and pro-inflammatory responses in human aortic endothelial cells

Cited by 99 publications

References 47 publications

Dietary Cocoa Butter or Refined Olive Oil Does Not Alter Postprandial hsCRP and IL‐6 Concentrations in Healthy Women

Dietary Cocoa Butter or Refined Olive Oil Does Not Alter Postprandial hsCRP and IL‐6 Concentrations in Healthy Women

Phenolic content of extra virgin olive oil is essential to restore endothelial dysfunction but not to prevent vascular inflammation in atherosclerotic lesions of Apo E deficient mice

Dynamic analysis of phospholipid metabolism of mouse macrophages treated with common non-steroidal anti-inflammatory drugs

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