Oleanolic acid, a natural triterpenoid improves blood glucose tolerance in normal mice and ameliorates visceral obesity in mice fed a high-fat diet

Melo, Célio L. de; Queiroz, Maria Goretti Rodrigues de; Fonseca, Said G C; Bizerra, Ayla Márcia Cordeiro; Lemos, Telma L. G.; Melo, Tiago Sousa de; Santos, Flávia Almeida; Rao, V. S. N.

doi:10.1016/j.cbi.2010.02.028

Cited by 124 publications

(89 citation statements)

References 32 publications

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“…Two representative anti-obesity drugs, Orlistat and Sibutramine, have been reported to increase in both AST and ALT levels in high fat diet-induced animal even if they have beneficial effects toward suppressing obesity. 24,25) Our result also showed the upregulation of AST in Orlistat-treated mice in accordance with previous literatures. In comparison, serum AST and ALT levels, the hepatotoxicity markers, were significantly decreased in D. oppositifolia-treated high-fat diet-fed mice at end of the study.…”

Section: Discussionsupporting

confidence: 93%

Anti-obesity Effect of <i>Dioscorea oppositifolia</i> Extract in High-Fat Diet-Induced Obese Mice and Its Chemical Characterization

Jeong

Jegal

Ahn

et al. 2016

Biological & Pharmaceutical Bulletin

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Dioscorea oppositifolia is a well-known edible and traditional medicine for the treatment of gastrointestinal diseases. In our previous study, D. oppositifolia exhibited both pancreatic lipase inhibition and an anti-adipogenesis effect in vitro. This study was performed to investigate the anti-obesity effect of D. oppositifolia on high-fat diet-induced obese mice. Female ICR mice were fed a high-fat diet with the 100 mg/ kg of D. oppositifolia n-BuOH extract for 8 weeks. The high-fat diet mice received the 15 mg/kg Orlistat orally as a positive control. The body weight, parametrial adipose tissue weight, and the levels of triglyceride (TG), total cholesterol (TC), and low density lipoprotein (LDL)-cholesterol in blood serum of female ICR mice were significantly decreased by feeding a high-fat diet with the n-BuOH extract of D. oppositifolia. An inhibitory effect of D. oppositifolia extract on dietary fat absorption was also clearly shown. The D. oppositifolia sample was found to contain 3,5-dimethoxy-2,7-phenanthrenediol and (3R,5R)-3,5-dihydroxy-1,7-bis(4-hydroxyphenyl)-3,5-heptanediol as main components based on its phytochemical analysis. The present study is the first report of the anti-obesity effect by D. oppositifolia n-BuOH extract using an established disease model. The increase in fecal fat excretion by treatment of D. oppositifolia may be an effective approach for treating obesity and related diseases.Key words Dioscorea oppositifolia; anti-obesity; high-fat diet-induced obese mouse; fecal fat excretion; phytochemical analysis Obesity is the disease associated with accumulation of excessive body fat resulting from energy imbalance.1) The obesity epidemic has become a public health problem, leading to secondary chronic diseases like dyslipidemia, cardiovascular disease, and type 2 diabetes.2,3) Furthermore, obese persons experience a number of physical problems such as sleep apnoea and joint pain, which shortens the life expectancy and impacts on the quality of life. 4) Natural phytochemicals have become more attractive as sources of industrial and medicinal materials in overweight and obese patients due to potential anti-obesity properties.5) Numerous trials have been conducted to discover pharmacological features of crude plant extracts, isolates, and phytochemical combinations against obesity. 6,7)Nevertheless, attention has continuously focused on new anti-obesity agents from herbal resources to minimize adverse effects associated with the present anti-obesity drugs. 6)Dioscorea oppositifolia L. (Dioscoreaceae, synonym: D. opposita THUNB.) has been cultivated in China, Japan, and Korea as a food and widely used as a traditional medicine for a long time. Our current pharmacological studies showed that D. oppositifolia possesses significant inhibitory activity against porcine pancreatic lipase in vitro. 8) In addition, the anti-adipogenesis effect of D. oppositifolia n-BuOH soluble extract has been detected in cultured 3T3-L1 adipocyte. 9)Biological activities of Dioscorea species against diverse met...

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Section: Discussionsupporting

confidence: 93%

Anti-obesity Effect of <i>Dioscorea oppositifolia</i> Extract in High-Fat Diet-Induced Obese Mice and Its Chemical Characterization

Jeong

Jegal

Ahn

et al. 2016

Biological & Pharmaceutical Bulletin

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“…Comparison of TTS and ethanol extract clearly reflects that antidiabetic activity of the extract is due to the major presence of a triterpenoid. Triterpenoid is well known for its antidiabetic activity (de Melo et al, 2010;Kazmi et al, 2012;Ramirez-Espinosa et al, 2011;Sarabu & Tilley, 2005). Triterpenoids can reduce blood glucose through increased insulin secretion, as evidenced in our experiment by STZ-induced diabetic rats, which is capable of modulating pancreatic secretion.…”

supporting

confidence: 55%

Antidiabetic potential of triterpenoid saponin isolated fromPrimula denticulate

Singh

Farswan²,

Ali³

et al. 2014

Pharmaceutical Biology

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Context: Primula denticulate Sm. (Primulaceae), commonly known as drumstick primula, is traditionally used to treat diabetes and urinary disorders. In the present study, a new triterpenoid saponin was isolated. Triterpenoids generally show antidiabetic activity. Considering its traditional use and chemical nature of the molecule, the present study was designed to evaluate the antidiabetic activity. Objective: Antidiabetic activity of triterpenoid saponin (TTS) isolated from P. denticulate. Materials and methods: A new TTS was isolated from the leaf of P. denticulate by column chromatography on CHCl 3 /MeOH (8.5:1.5) fraction. It was further characterized by using NMR, UV, and IR spectroscopic methods. Ethanol and aqueous extracts of the leaf were also prepared. Antidiabetic study for TTS, ethanol extract, and aqueous extract was carried out in streptozotocin (STZ)-induced diabetic rats at doses of 200, 1000, and 1000 mg/kg body weight, respectively. A toxicity study was also performed. -hydroxy-13b,28-epoxy-olean-30-al by NMR, UV, and IR spectroscopic methods. This new TTS was found to be effective in lowering blood-glucose level in the experimental rat model, thus establishing its antidiabetic property (168.8 AE 4.58) when compared with disease control (258.8 AE 0.60). Its LD 50 value was found at a dose of 2000 mg/kg. The level of insulin was restored by TTS and ethanol extract up to 31.49 mU/ml and 38.90 mU/ml, respectively, when compared with disease control (18.45 mU/ml). Discussion and conclusion: Results: Isolated new TTS molecule was characterized as 3-arabinopyranosyloxy]-16a-hydroxy-3b,28-epoxy-olean-30-al possesses potential glucose lowering properties, i.e., antidiabetic potential against STZ-induced diabetic rats.

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“…Inulin (from chicory, obtained from Sigma-Aldrich, St. Louis, MO, USA) was dissolved at 20 % in distilled water and 1mL of this solution was given to each mouse by gavage. The high-fat diet was prepared according to previously reported methods [14,15] and consisted of 150 g of mouse pellet standard chow, 100 g roasted peanuts, 100 g milk chocolate, 50 g sesame crackers and 20 g roasted sesame (Table 1). In addition, the obese group was fed 420 g creamy biscuits (1388 cal) weekly.…”

Section: Experimental Animals and Experimental Protocolmentioning

confidence: 99%

Effect of inulin supplementation in male mice fed with high fat diet on biochemical profile and α-amylase gene expression

Pouyamanesh¹,

Amoli²,

Yaghmaei³

et al. 2016

Trop. J. Pharm Res

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TG (282.15 ± 1.83 mg/dl in O2, (p < 0.001)), and cholesterol levels (335.72 ± 2.23 mg/dl in O2, (p < 0.001)), compared with control. In C2 group, mean body weight was 25.71 ± 0.54 g, TG level 214.29 ± 5.54 mg/dl, and cholesterol level 164.29 ±4.57 mg/dl. Compared to obese group, mice receiving inulin showed lower blood glucose levels (223.10 ± 8.7 mg/dl in E2, p < 0.001), body weight (27.86 ± 0.57 g in E2, p < 0.001), TG (232.14 ± 4.02 mg/dl in E2, p < 0.001) and cholesterol (249.97 ± 2.28 in E2, p < 0.001

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Oleanolic acid, a natural triterpenoid improves blood glucose tolerance in normal mice and ameliorates visceral obesity in mice fed a high-fat diet

Cited by 124 publications

References 32 publications

Anti-obesity Effect of <i>Dioscorea oppositifolia</i> Extract in High-Fat Diet-Induced Obese Mice and Its Chemical Characterization

Anti-obesity Effect of <i>Dioscorea oppositifolia</i> Extract in High-Fat Diet-Induced Obese Mice and Its Chemical Characterization

Antidiabetic potential of triterpenoid saponin isolated fromPrimula denticulate

Effect of inulin supplementation in male mice fed with high fat diet on biochemical profile and α-amylase gene expression

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