Of humans and canines: A comparative evaluation of heat shock and apoptosis-associated proteins in mammary tumors

Ramasamy, Kumaraguruparan; Karunagaran, Devarajan; Balachandran, C.; Manohar, B. Murali; Nagini, Siddavaram

doi:10.1016/j.cca.2005.08.018

Cited by 42 publications

(55 citation statements)

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“…High temperature or heat stress has been found to induce an increase in HSP protein production in certain tissues [22]. HSP 70 stimulates proliferation of some types of tumor cells [6], and it has been found to be an anti-apoptotic protein that inhibits cell death by apoptosis in human and canine mammary gland tumor cells [18] and human prostatic adenocarcinama cells [20]. The high level of HSP 70 in canine SCTs maintains the viability and development of tumor cells.…”

Section: Discussionmentioning

confidence: 99%

“…A high ROS concentration in the cryptorchid testis is an important risk factor for the occurrence and development of testicular tumors [29]. Heat shock protein (HSP) 70 is the main protein produced abundantly in some tissues exposed to heat stress [22], and it induces the development of certain tumors [6,18,20]. Inhibin (INH) in male animals is a glycoprotein produced by Sertoli cells [23,24,28] and is composed of two subunits, an α-unit and a β-unit [8,23].…”

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confidence: 99%

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Testicular Superoxide Dismutase Activity, Heat Shock Protein 70 Concentration and Blood Plasma Inhibin-.ALPHA. Concentration of Dogs with a Sertoli Cell Tumor in a Unilateral Cryptorchid Testis

et al. 2007

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ABSTRACT. The proportions of Sertoli cell tumor (SCT), seminoma and Leydig cell tumor in 50 dogs with unilateral testicular tumors were 52%, 36% and 12%, respectively. The rate of occurrence of SCT in the cryptorchid testis was very high (71%). The testicular superoxide dismutase (SOD) activity, testicular heat shock protein (HSP) 70 concentration and peripheral blood plasma inhibin (INH)-α concentration of 10 dogs with a unilateral cryptorchid testis and no testicular tumors, 10 dogs with SCT in a unilateral cryptorchid testis and 10 normal dogs, all aged 5-15 years, were measured in order to identify high risk factors for the occurrence of SCT in the canine cryptorchid testis. The mean SOD activity in cryptorchid testes and SCTs was significantly lower and higher, respectively, than in normal testes (both P<0.01). The mean HSP 70 concentration in both cryptorchid testes and SCTs was significantly higher than in normal testes (both P<0.01). The mean plasma INH-α concentration of the cryptorchid and SCT dogs was significantly lower and higher, respectively, than in normal dogs (P<0.05 and 0.01, respectively). The low SOD activity in the cryptorchid testis, low blood plasma INH-α concentration of the cryptorchid dogs and high HSP 70 concentration in the SCTs may be related to the occurrence of SCT and tumor cell proliferation in canine cryptorchid testes.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Testicular Superoxide Dismutase Activity, Heat Shock Protein 70 Concentration and Blood Plasma Inhibin-.ALPHA. Concentration of Dogs with a Sertoli Cell Tumor in a Unilateral Cryptorchid Testis

et al. 2007

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“…Similar studies were conducted by Storm (1996), who stated that tumors with Hsp27 expression showed a higher histological grade than tumors negative for the expression of this protein (Storm et al, 1993). Kumaraguruparan (2006) studied the expression of Hsp70 and Hsp90 in breast cancer in women and found a correlation between the expression of both proteins and proliferative activity (Kumaraguruparan et al, 2006). He suggested that heat shock proteins are important prognostic factors.…”

Section: Discusionmentioning

confidence: 63%

12_Prospective Study of Tumor Markers as Prognostic Factors in the Histopathological Differential Diagnosis of Mammary Gland Neoplasms in Female Canines

Badowska-Kozakiewicz¹

2012

A Bird's-Eye View of Veterinary Medicine

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“…In CMT, Yang et al [14] reported that the levels of cell proliferation and apoptosis did not appear to be correlated with the expression of Bcl-2 and Bcl-2 expression were slightly greater in benign CMTs than in their malignant counterparts. There are data stating that while expression of Bcl-2 and Bcl-X L were increased, expression of Bax was significantly lower in both human breast cancer and CMT tissues compared to corresponding adjacent tissue [15] . Although apoptosis-associated proteins were extensively studied in human breast cancer, there are few studies on CMT.…”

Section: Introductionmentioning

confidence: 99%

Köpek Meme Tümörlerinde Bcl-2, Bcl-XL ve Bax Sunulumu İle Apoptotik İndeksin Değerlendirilmesi

Yıldırım¹,

Sönmez²,

Özyoğurtçu³

et al. 2014

Kafkas Univ Vet Fak Derg

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SummaryMammary tumours are the most common neoplasms in intact female dogs. Dysregulation of programmed cell death mechanisms plays an important role in the pathogenesis and progression of mammary gland tumours. The aim of this study was to investigate the relationship between some anti-apoptotic proteins (Bcl-2, Bcl-X L and Bax), apoptotic index (AI) and histopathological diagnosis, tumour grading, tumour staging and survival time of canine mammary tumours (CMT). Twenty seven tissue samples were collected from twenty seven animals with mammary tumours. The samples were evaluated and graded histopathologically. All cases were staged according to the TNM system. The expression of Bcl-2, Bcl-X L and Bax proteins was investigated using indirect immunoperoxidase test and apoptosis was evaluated using terminal deoxynucleotidyltransferase (TdT)-mediated nick end-labelling (TUNEL) technique. Follow-up examination and survival estimation analysis were performed. While there was a significant statistical relation between Bcl-2 expression and histopathological diagnosis (P<0.005), there was no considerable association between histopathological diagnosis and Bax, Bcl-X L and AI (P>0.05). The differences between T1 and T5, T2 and T5 stages were statistically significant in terms of Bax expression (P<0.05), and Bax expressions were higher in T5 when compared with T1 or T2. No association between survival time and Bcl-2, Bax, Bcl-X L and AI was determined (P>0.05). Bcl-2 was overexpressed in highly malignant tumours such as solid and tubulopapillary adenocarcinomas and Bax had high expression levels in metastatic tumours. As a result, it is concluded that Bcl-2 and Bax expression can be accessory parameters for anticipating the biologic behaviour and prognosis of CMT but these markers alone are not sufficient for the determination of survival time. Keywords ÖzetMeme tümörleri intakt dişi köpeklerin en sık karşılaşılan tümörleridir. Programlı hücre ölüm mekanizmalarında meydana gelen düzensizlikler meme bezi tümörlerinin patogenezis ve progresyonunda önemli bir rol oynamaktadır. Bu çalışmanın amacı köpek meme tümörlerinde (KMT) bazı anti-apoptotik proteinler (Bcl-2, Bcl-X L and Bax) ve apoptotik indeksin (AI), histopatolojik tanı, tümör derecelendirmesi, tümör evreleri ve kalan yaşam süreleri ile ilişkisini ortaya koymaktır. Meme tümörü olan yirmi yedi hayvandan yirmi yedi biyopsi örneği toplandı. Örnekler histopatolojik olarak incelendi ve derecelendirildi. Bütün olgular TNM sistemine göre evrelendi. Bcl-2, Bcl-X L ve Bax proteinlerinin sunulumu indirect immunoperoksidaz testi ile ve apoptozis ise terminal deoksinükleotidiltransferaz (TdT)-aracılı nick end-labelling (TUNEL) tekniği ile incelendi. Hasta takibi ve hayatta kalma süresi tahmini analizleri gerçekleştirildi. Bcl-2 sunulumu ve histopatolojik tanı arasında önemli bir istatistiksel ilişki belirlenirken (P<0.005), histopatolojik tanı ile Bax, Bcl-X L ve AI arasında dikkate değer bir ilişki saptanmadı (P>0.05). Bax sunulumu açısından T1 ve T5 ile T2 ve T5 evreleri aras...

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Of humans and canines: A comparative evaluation of heat shock and apoptosis-associated proteins in mammary tumors

Cited by 42 publications

References 31 publications

Testicular Superoxide Dismutase Activity, Heat Shock Protein 70 Concentration and Blood Plasma Inhibin-.ALPHA. Concentration of Dogs with a Sertoli Cell Tumor in a Unilateral Cryptorchid Testis

Testicular Superoxide Dismutase Activity, Heat Shock Protein 70 Concentration and Blood Plasma Inhibin-.ALPHA. Concentration of Dogs with a Sertoli Cell Tumor in a Unilateral Cryptorchid Testis

12_Prospective Study of Tumor Markers as Prognostic Factors in the Histopathological Differential Diagnosis of Mammary Gland Neoplasms in Female Canines

Köpek Meme Tümörlerinde Bcl-2, Bcl-XL ve Bax Sunulumu İle Apoptotik İndeksin Değerlendirilmesi

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