Ocurrence and caracterization of bovine abortion caused by Brucella abortus infection in southern Brazil

Abstract

Ocurrence and caracterization of bovine abortion caused by Brucella abortus infection in southern Brazil RESUMENLa brucelosis causada por Brucella abortus es una enfermedad zoonótica contagiosa que causa abortos en las vacas, así como alteraciones articulares y reproductivos en los toros. En 469 fetos bovinos abortados analizados en un período de nueve años, nueve (1,92%) se debieron a B. abortus infección. El diagnóstico se basó en los estudios de lesiones macroscópicas, histológicas, microbiológicas, serológicas, inmunohistoquímicas y hallazgos moleculares. La edad gestacional de los fetos osciló de 6 a 9 meses. Los principales hallazgos macroscópicos se caracterizaron por una descarga de pus en la cavidad nasal, tráquea y bronquios, y depósitos de fibrina pleural y pericárdico, además de los pulmones se han observado manchas blancas en el parénquima y las zonas rojas en los lóbulos apicales. En la región intercotiledonaria de tres placentas examinadas, hubo áreas enrojecidas intercaladas con zonas redondas de color amarillo-blanco bien definidos, con paredes engrosadas en la superficie de los cotiledones. Microscópicamente se observó bronconeumonía necrosupurativa, neumonía intersticial y placentitis necrosupurativa con áreas de vasculitis, la mineralización, y la trombosis. Brucella abortus fue aislado de los tejidos fetales de siete de los nueve casos investigados. La prueba de inmunohistoquímica fue positiva en cuatro casos, y la PCR amplifica el material genético de las bacterias en tres. La infección con B. abortus tiene que ser considerado como una posible causa de aborto en el ganado en el sur de Brasil.Palabras clave: brucelosis, diagnóstico, ganado, fetos bovinos. SUMMARYBrucellosis caused by Brucella abortus infection is a contagious zoonotic disease that causes abortions in cows, as well as joint and reproductive alterations in bulls. This study reports that eight (1.92%) out of a total of 469 aborted bovine fetuses analysed in a time frame of nine years were caused by B. abortus infection. The diagnosis was based on macroscopic, histological, microbiological, serological, immunohistochemical, and molecular findings. The gestational age of the fetuses studied ranged from 6 to 9 months. The main gross findings were purulent discharge from the nasal cavity, trachea and bronchi; pleural and pericardial fibrin deposits; and non-collapsed lungs with white spots in the parenchyma, and red areas in the apical lobes. Intercotyledonary areas of three placentas examined exhibited reddened areas, and the cotyledons exhibited well-defined, yellowish-white round areas with thickened walls on their surface. Microscopically, the most frequently observed lesions were necrosuppurative bronchopneumonia, interstitial pneumonia, and necrosuppurative placentitis with areas of vasculitis, mineralization, and thrombosis. Brucella abortus was isolated from fetal tissues of seven of the nine cases investigated. Immunohistochemical tests were positive in four cases, and PCR detected B. abortus genetic material in three case...

Research Article Detection of <i>Brucella</i> <i>abortus</i> DNA in the reproductive tract of buffalo (<i>Bubalus</i> <i>bubalis</i>) cows

“…This conclusion agrees with previous studies using buffaloes experimentally inoculated with B. abortus (Sousa et al, 2015a;2015b), and studies of bovine abortions caused by Brucella spp. that can invade host epithelial cells and allow infection through mucosal surfaces (Poester et al, 2013 andAntoniassia et al, 2016). It was possible to detect Brucella DNA from swab samples of the uterine mucus, similar to what has been observed in dogs, where the use of a swab is minimally invasive, avoiding stress in the animals (Fernandes et al, 2013).…”

Trueperella pyogenes como causa de aborto em uma vaca - relato de caso

“…As lesões histológicas também são semelhantes às de aborto por brucelose, tais como pneumonia necrossupurativa e pleurite fibrinosa. As infiltrações de células inflamatórias no fígado e a placentite purulenta também são características em comum entre as duas bactérias (Orlando et al, 2014;Antoniassi et al, 2016).…”

Verminotic pneumonia in South American fur seal (Arctocephalus australis) in Southern Brazil

“…Antigen retrieval was achieved with Proteinase K (Dakocytomation, Carpinteria, CA, USA) for 10 min and the reactions were revealed with chromogen 3-amino-9-ethylcarbazole (AEC Romulin; Biocare, Pacheco, USA). As a positive control, lung slides from a bovine fetus infected with B. abortus (Antoniassi et al ., 2016 ) were used and negative controls were incubated with commercial universal serum (Universal Negative Control Serum; Biocare, Pacheco, USA).…”