Oct-4: Control of totipotency and germline determination

Repression of Ets-2-Induced Transactivation of the Tau Interferon Promoter by Oct-4

“…GST-Oct-4 and various truncation products were coupled to glutathione-Sepharose beads and tested for their abilities to trap Ets-2 that had been labeled with [ 35 S]methionine by in vitro translation. As expected, 35 S-labeled Ets-2 did form a complex with full-length GST-Oct-4 (Fig. 6A).…”

A fully automated FAIMS-DIA proteomic pipeline for high-throughput characterization of iPSC-derived neurons

“…Next, we used the FAIMS (-35V)-DIA datasets to assess the efficiency of neuronal differentiation efficacy in KOLF2.1J-derived Neurons (Supplemental Table S2). As expected, protein expression of pluripotency and neural precursor markers POU5F1 (OCT4) (Pesce & Scholer, 2000), PODXL (TRA-1-60) (Draper et al, 2002;Henderson et al, 2002), and SOX2 (Botquin et al, 1998;Ellis et al, 2004) were significantly reduced at the early stage of neuron differentiation (Fig. 5A).…”

Errata

“…Oct4, a transcriptional factor, has been suggested as an embryonic stem cell marker 4. Although one study showed Oct4 gene expression in human tumors but not in normal somatic tissues 5, we found that Oct4 nuclear expression is strongly associated with benign lesions but not prostate cancer.…”