Ocorrência e fatores de risco associados às infecções por Toxoplasma gondii e Neospora caninum em cães no município de Natal, Estado do Rio Grande do Norte, Nordeste do Brasil

Dantas, Sabrina Barros Araújo; Fernandes, Annielle Regina da Fonseca; Neto, Orestes Luiz de Souza; Mota, Rinaldo Aparecido; Alves, Clebert José; Azevêdo, Sérgio Santos de

doi:10.1590/s0103-84782013001100020

Cited by 19 publications

(21 citation statements)

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“…The IFAT results show clearly that there was a higher risk of infection with increasing age, especially among dogs older than 2 years, even though there was no statistical association. These results emphasize that the likelihood of infection increases with the dogs' ages and is in agreement with previous studies (CÃNÓN-FRANCO et al, 2004;DANTAS et al, 2013;LANGONI et al, 2013). In the present study, there was a significant difference regarding dogs seropositive for T. gondii between those with and without a defined breed (p<0.05), which was in agreement with Moura et al (2009).…”

Section: Discussionsupporting

confidence: 93%

“…The high frequency of anti-T. gondii antibodies in dogs (63.7% according to ELISA and 57.4% from IFAT) observed in this study corroborates other Brazilian findings: Minervino et al (2012) in Mato Grosso (52%), Valadas et al (2010) in Pará (69.8%) and Cañón-Franco et al (2004) in Rondônia (76.4%). However, lower values for anti-T. gondii antibodies were observed in other Brazilian states, by Dantas et al (2013) in Rio Grande do Norte (11.5%) and Lopes et al (2011) in Piauí (18%). The data corresponding to the frequency of T. gondii in Brazil are discordant, which may be related to different serological tests, sample sizes, types of dog population (DUBEY & SCHARES, 2011).…”

Section: Discussionmentioning

confidence: 62%

“…These results are probably related to the type of management to which dogs without a defined breed are subjected. However, Dantas et al (2013) and Langoni et al (2013) did not observe any association between seropositivity and breed. The lack of statistical association between activity area, consumption of hunted meat, presence of other pets and cats in the household and presence of antibodies against T. gondii was not expected, since dogs kept in this context would theoretically have greater exposure to sources of infection.…”

Section: Discussionmentioning

confidence: 83%

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Toxoplasma gondii and Neospora caninum in dogs from the state of Tocantins: serology and associated factors

Raimundo

Guimarães

Moraes

et al. 2015

Rev. Bras. Parasitol. Vet.

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This study investigated occurrences of anti-Neospora caninum and anti-Toxoplasma gondii IgG antibodies by means of the enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence assay (IFAT), along with risk factors associated with toxoplasmosis and neosporosis, in 204 dogs from urban and rural areas of the municipality of Araguaína, state of Tocantins, Brazil. One hundred and thirty samples (63.7%) were positive for T. gondii using ELISA: 57.1% and 70.7% in the urban and rural areas, respectively. The seropositivity frequency for T. gondii observed through IFAT was 57.4%, distributed between rural and urban areas as 62.6% and 52.4%, respectively. The factors associated with canine toxoplasmosis were age and breed (p<0.05). In relation to N. caninum, 88 samples (43.1%) were positive, according to ELISA, distributed as 42.9% in urban areas and 43.3% in rural areas. Anti -N. caninum antibodies were detected through IFAT in 62 dogs (30.4%), distributed as 31.3% and 29.5% between rural and urban areas, respectively. Age and breed were associated with neosporosis occurrence (p<0.05) by IFAT. This study provides the first detection of IgG antibodies for canine toxoplasmosis and neosporosis in the state of Tocantins and highlights the importance of dogs in the epidemiological chain of these diseases.Keywords: Dogs, toxoplasmosis, neosporosis, ELISA, IFAT. ResumoEste estudo investigou a ocorrência de anticorpos anti-Neospora caninum e anti-Toxoplasma gondii por ensaio imunoenzimático indireto (ELISA) e reação de imunofluorescência indireta (RIFI), assim como os fatores de risco associados à toxoplasmose e à neosporose em 204 cães provenientes de áreas urbana e rural do município de Araguaína, Estado de Tocantins, Brasil. Cento e trinta amostras (63,7%) foram positivas para T. gondii, destas 57,1% e 70,7% oriundas de áreas urbanas e rurais, respectivamente. Considerando-se o teste RIFI, a frequência de soropositividade para T. gondii foi de 57,4% com distribuição de 62,6% e 52,4% entre áreas rurais e urbanas, respectivamente. Fatores associados à toxoplasmose canina foram raça e idade, com soropositividade maior para animais mais velhos (p<0,05). Em relação à N. caninum, 88 (43,1%) amostras foram positivas, segundo ELISA, sendo distribuídas em 42,9% para área urbana e 43,3% para área rural. Por meio da RIFI, anticorpos anti-N. caninum foram detectados em 62 (30,4%) cães, sendo distribuídos em 31,3% e 29,5% entre áreas rurais e urbanas, respectivamente. Os fatores associados à ocorrência de neosporose, pela RIFI, foram idade e raça (p<0,05). Este estudo representa a primeira detecção de anticorpos IgG para toxoplasmose e neosporose canina no Estado de Tocantins e evidencia a importância de cães na cadeia epidemiológica dessas doenças.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 83%

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Toxoplasma gondii and Neospora caninum in dogs from the state of Tocantins: serology and associated factors

Raimundo

Guimarães

Moraes

et al. 2015

Rev. Bras. Parasitol. Vet.

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“…Contact with soil and pets was regarded as an important risk factor for T. gondii infection, which also affects about 65% of pregnant women in this region [16][17][18]. These data are consistent with reports from other Brazilian regions [5,[19][20][21].…”

Section: Introductionsupporting

confidence: 87%

Toxoplasmose ocular com reação em cadeia da polimerase positiva em sangue periférico – relato de dois casos, estado de São Paulo, Brasil

Barbosa¹,

Frederico²,

Previato³

et al. 2016

Sci Med

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Aims:To describe the use of polymerase chain reaction (PCR) in peripheral blood and demonstrate its importance in the clinical follow-up of patients with ocular toxoplasmosis. Case description: Two immunocompetent patients were clinically diagnosed with acute ocular toxoplasmosis. The routine clinical evaluation consisted of fundus examination using binocular indirect ophthalmoscopy, color fundus photography, fluorescein angiography, and spectral domain optical coherence tomography. The serological diagnosis was made by enzyme-linked immunosorbent assay (ELISA) and confirmed by enzyme-linked fluorescent assay (ELFA). The molecular diagnosis was made by PCR in peripheral blood using the B1 gene of Toxoplasma gondii as marker. The younger patient was male, had previous lesion in the right eye, complained of low visual acuity in the left eye and was under treatment. The older patient was male, had retinal detachment, and presented with sudden loss of acuity in the right eye. The fundus examination revealed chorioretinal scar in the left eye. IgG was reactive, IgM was non-reactive, and PCR was positive in the peripheral blood of both patients. New blood samples were collected for serological and molecular monitoring and PCR remained positive in both cases. Six weeks after treatment with oral sulfadiazine and pyrimethamine, the PCR yielded negative results. Conclusions:The results show that T. gondii antigens may be found in peripheral blood during ocular reactivations and that PCR may be a good tool for the follow-up of patients with ocular toxoplasmosis.KEY WORDS: Toxoplasma gondii; toxoplasmosis, ocular; retinochoroiditis; chorioretinitis; retinography; optical coherence tomography; PCR; polymerase chain reaction RESUMO Objetivos: Descrever o uso da reação em cadeia da polimerase (PCR) no sangue periférico e demonstrar sua importância no acompanhamento clínico de pacientes com toxoplasmose ocular. Descrição dos casos: Dois pacientes imunocompetentes foram clinicamente diagnosticados com toxoplasmose ocular aguda. Rotineiramente, a avaliação clínica foi feita por fundoscopia com o uso de oftalmoscópio binocular indireto, retinografia colorida, angiografia fluorescente e tomografia de coerência óptica espectral. A sorologia foi realizada por ensaio imunoenzimático (ELISA) e confirmada por ensaio imunoenzimático fluorescente ELFA (IgG, IgM). O diagnóstico molecular foi realizado por PCR em sangue periférico usando o gene B1 de Toxoplasma gondii como marcador. O paciente mais jovem era do sexo masculino, apresentava lesão prévia no olho direito, queixa de baixa acuidade visual no olho esquerdo e estava sob tratamento. O paciente mais velho era do sexo masculino, apresentava descolamento de retina e súbita diminuição de visão no olho direito. A fundoscopia revelou cicatriz coriorretiniana no olho esquerdo. Ambos os pacientes tinham IgG reagente, IgM não reagente e PCR positivo em sangue periférico. Novas amostras de sangue foram coletadas para monitoramento sorológico e molecular e a PCR permaneceu positiva e...

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“…Estudos recentes no Brasil mostram uma variação na frequência de soropositividade para T. gondii em cães de 11,50% a 88,52% (16)(17)(18)(19)(20)(21) . Essa variação de resultados pode estar relacionada às diferentes técnicas utilizadas e pontos de corte.…”

Section: Discussionunclassified

Frequência De Anticorpos Anti-Toxoplasma Gondii Em Cães Com Sinais Clínicos Compatíveis Com Toxoplasmose

et al. 2016

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Abstract:The aim of this study was to detect antibodies against T. gondii in dogs with clinical signs compatible with toxoplasmosis attended in a veterinary hospital. A serological survey was performed in 598 dogs from 1993 to 2013. The IgG antibodies survey against T. gondii was carried out by means of Indirect Fluorescence Antibodies Test (IFAT); samples with titers ≥16 were considered positive. Among the dogs with clinical signs, 259 (43,31%) were positive for T. gondii with titers varying between 16 to 4096; among the healthy animals, 79 (22.25%) were reagents. The most frequent signals were: neurological (30.43%) and uveitis (8.03%). Among variables race, gender, and age, only the last one affected the observed results with greater frequency f seropositive in elderly dogs. Keywords: clinical signs; dogs; IFAT; serology; Toxoplasma gondii; zoonosis. IntroduçãoToxoplasma gondii é um protozoário intracelular obrigatório que apresenta distribuição cosmopolita e caráter oportunista. Os animais homeotérmicos são considerados hospedeiros intermediários e os felídeos, os definitivos; nestes os oocistos do parasito se desenvolvem e são eliminados com as

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Ocorrência e fatores de risco associados às infecções por Toxoplasma gondii e Neospora caninum em cães no município de Natal, Estado do Rio Grande do Norte, Nordeste do Brasil

Cited by 19 publications

References 16 publications

Toxoplasma gondii and Neospora caninum in dogs from the state of Tocantins: serology and associated factors

Toxoplasma gondii and Neospora caninum in dogs from the state of Tocantins: serology and associated factors

Toxoplasmose ocular com reação em cadeia da polimerase positiva em sangue periférico – relato de dois casos, estado de São Paulo, Brasil

Frequência De Anticorpos Anti-Toxoplasma Gondii Em Cães Com Sinais Clínicos Compatíveis Com Toxoplasmose

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