Dissolved organic carbon (DOC) represents a heterogeneous group of naturally-occurring molecules in aquatic environments, and recent studies have evidenced that optically dark DOCs can exert some positive effects on ionoregulatory homeostasis of aquatic organisms in acidic waters. We investigated the effects of Luther Marsh DOC, a dark allochthonous DOC, on ion regulation and N-waste excretion of zebrafish acutely exposed to either neutral or low pH in ion-poor water. In the first experiment, simultaneous exposure to pH 4.0 and DOC greatly attenuated the stimulation of Na diffusive losses (J ), and prevented the blockade of Na uptake (J ) seen in zebrafish exposed to pH 4.0 alone, resulting in much smaller disturbances in Na net losses (J ). DOC also attenuated the stimulation of net Cl losses (J ) and ammonia excretion (J ) during acidic challenge. In the second experiment, zebrafish acclimated to DOC displayed similar regulation of J and J , and, therefore, reduced J at pH 4.0, effects which persisted even when DOC was no longer present. Protective effects of prior acclimation to DOC on J and J at pH 4.0 also occurred, but were less marked than those on Na balance. Urea fluxes were unaffected by the experimental treatments. Overall, these effects were clearly beneficial to the ionoregulatory homeostasis of zebrafish at low pH, and were quite similar to those seen in a recent parallel study using darker DOC from the upper Rio Negro. This suggests that dark allochthonous DOCs share some chemical properties that render fish tolerant to ionoregulatory disturbances during acidic challenge.