Ochratoxin A kinetics: A review of analytical methods and studies in rat model

Vettorazzi, Ariane; González-Peñas, Elena; Ceráin, Adela López de

doi:10.1016/j.fct.2014.07.020

Cited by 38 publications

(22 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For the interpretation of in vivo data from toxicological reports an understanding of the kinetics of ochratoxins is crucial, including the absorption, distribution, metabolism, and excretion of the toxins [ 65 ]. The toxicokinetics of OTA and their toxicological implications for animals and humans have been extensively reviewed by various authors [ 7 , 39 , 53 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 ]. For example, the available kinetic data for rats indicate that strain, sex and age differences in OTA kinetics may account for the different sex and species sensitivities towards OTA [ 69 ].…”

Section: Occurrence Of Ochratoxinsmentioning

confidence: 99%

Comparative Ochratoxin Toxicity: A Review of the Available Data

Heussner

Bingle

2015

Toxins

267

160

View full text Add to dashboard Cite

Ochratoxins are a group of mycotoxins produced by a variety of moulds. Ochratoxin A (OTA), the most prominent member of this toxin family, was first described by van der Merwe et al. in Nature in 1965. Dietary exposure to OTA represents a serious health issue and has been associated with several human and animal diseases including poultry ochratoxicosis, porcine nephropathy, human endemic nephropathies and urinary tract tumours in humans. More than 30 years ago, OTA was shown to be carcinogenic in rodents and since then extensive research has been performed in order to investigate its mode of action, however, this is still under debate. OTA is regarded as the most toxic family member, however, other ochratoxins or their metabolites and, in particular, ochratoxin mixtures or combinations with other mycotoxins may represent serious threats to human and animal health. This review summarises and evaluates current knowledge about the differential and comparative toxicity of the ochratoxin group.

show abstract

Section: Occurrence Of Ochratoxinsmentioning

confidence: 99%

Comparative Ochratoxin Toxicity: A Review of the Available Data

Heussner

Bingle

2015

Toxins

267

160

View full text Add to dashboard Cite

show abstract

“…OTA is mainly detoxified into ochratoxin α (OTα) by animal tissues and by intestinal anaerobic bacteria. Other metabolites of OTA are formed in the liver by cytochrome P450 and these metabolites differ from one animal species to another [13]. OTA and OTα are excreted in faeces, whereas other OTA metabolites, together with OTA and OTα, are found in urine [14].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Evaluation of the Efficiency of Yeast Cell Wall Extract to Adsorb Ochratoxin A and Mitigate Accumulation of the Toxin in Broiler Chickens

Vartiainen

Yiannikouris

Apajalahti

et al. 2020

Toxins

View full text Add to dashboard Cite

Ochratoxin A (OTA) is a common mycotoxin contaminant in animal feed. When absorbed from the gastrointestinal tract, OTA has a propensity for pathological effects on animal health and deposition in animal tissues. In this study, the potential of yeast cell wall extracts (YCWE) to adsorb OTA was evaluated using an in vitro method in which consecutive animal digestion events were simulated. Low pH markedly increased OTA binding to YCWE, which was reversed with a pH increased to 6.5. Overall, in vitro analysis revealed that 30% of OTA was adsorbed to YCWE. Additional computational molecular modelling revealed that change in pH alters the OTA charge and modulates the interaction with the YCWE β-d-glucans. The effectiveness of YCWE was tested in a 14-day broiler chicken trial. Birds were subjected to five dietary treatments; with and without OTA, and OTA combined with YCWE at three dosages. At the end of the trial, liver OTA deposition was evaluated. Data showed a decrease of up to 30% in OTA deposits in the liver of broilers fed both OTA and YCWE. In the case of OTA, a tight correlation between the mitigation efficacy of YCWE between in vitro and in vivo model could be observed.

show abstract

“…Biomarker-based approaches are more and more used to assess dietary exposure, as they allow to analyze physiological samples like urine or blood for each test person individually [4]. Various sample preparation and quantification techniques are described for mycotoxins and their metabolites in several physiological samples [5–9]. Main challenges in biomonitoring-based methods are usually low analyte concentrations and matrix interferences during analysis.…”

Section: Introductionmentioning

confidence: 99%

Multi-mycotoxin analysis using dried blood spots and dried serum spots

et al. 2017

View full text Add to dashboard Cite

In this study, a rapid multi-mycotoxin approach was developed for biomonitoring and quantification of 27 important mycotoxins and mycotoxin metabolites in human blood samples. HPLC-MS/MS detection was used for the analysis of dried serum spots (DSS) and dried blood spots (DBS). Detection of aflatoxins (AFB1, AFB2, AFG1, AFG2, AFM1), trichothecenes (deoxynivalenol, DON; DON-3-glucoronic acid, DON-3-GlcA; T-2; HT-2; and HT-2-4-GlcA), fumonisin B1 (FB1), ochratoxins (OTA and its thermal degradation product 2’R-OTA; OTα; 10-hydroxychratoxin A, 10-OH-OTA), citrinin (CIT and its urinary metabolite dihydrocitrinone, DH-CIT), zearalenone and zearalanone (ZEN, ZAN), altenuene (ALT), alternariols (AOH; alternariol monomethyl ether, AME), enniatins (EnA, EnA1, EnB, EnB1) and beauvericin (Bea) was validated for two matrices, serum (DSS), and whole blood (DBS). HPLC-MS/MS analysis showed signal suppression as well as signal enhancement due to matrix effects. However, for most analytes LOQs in the lower pg/mL range and excellent recovery rate were achieved using matrix-matched calibration. Besides validation of the method, the analyte stability in DBS and DSS was also investigated. Stability is a main issue for some analytes when the dried samples are stored under common conditions at room temperature. Nevertheless, the developed method was applied to DBS samples of a German cohort (n = 50). Besides positive findings of OTA and 2’R-OTA, all samples were positive for EnB. This methodical study establishes a validated multi-mycotoxin approach for the detection of 27 mycotoxins and metabolites in dried blood/serum spots based on a fast sample preparation followed by sensitive HPLC-MS/MS analysis. Graphical Abstractᅟ Electronic supplementary materialThe online version of this article (doi:10.1007/s00216-017-0279-9) contains supplementary material, which is available to authorized users.

show abstract

Ochratoxin A kinetics: A review of analytical methods and studies in rat model

Cited by 38 publications

References 89 publications

Comparative Ochratoxin Toxicity: A Review of the Available Data

Comparative Ochratoxin Toxicity: A Review of the Available Data

Comprehensive Evaluation of the Efficiency of Yeast Cell Wall Extract to Adsorb Ochratoxin A and Mitigate Accumulation of the Toxin in Broiler Chickens

Multi-mycotoxin analysis using dried blood spots and dried serum spots

Contact Info

Product

Resources

About