Occurrence of IL-1, IL-10, CD25, CD40, and CD69 in the tissue of palatine tonsils

Abstract

Introduction Palatine tonsil disease often coexists with dermatological diseases. Correct diagnosis of inflammation of the palatine tonsil tissue and removal of the diseased palatine tonsils results in remission of the disease. Aim To determine similarities and differences in the immunohistochemistry profile of the palatine tonsil tissue between tonsillitis and hypertrophy, including location of the immunohistochemistry reactions in specific histological sites. Material and methods A prospective analysis of 50 palatine tonsils that had undergone tonsillectomy due to tonsillitis (30 cases) and hypertrophy (20 cases) was performed. The collected material underwent immunohistochemistry staining for: IL-1, IL-10, CD25, CD40, and CD69, and subsequently phenotypic expression of the obtained results was performed including their histological location. Results Statistically significant differences ( p < 0.05) between the tonsillitis and hypertrophy groups were found for almost all IHC reactions in the epithelium covering the tonsils for CD-25, CD-69, IL-1, IL-10. Furthermore, significant differences between these groups were found for IL-10 reaction in the subepithelial inflammatory infiltrate and follicular centres of lymphatic follicles as well as for CD-69 reaction between the follicles. When all the locations were summarized, significant ( p < 0.05) differences were found for all IHC reactions except for CD-40. Conclusions The investigated markers and cytokines: CD25 and CD69, and IL-1 and IL-10 are more abundant in tonsillitis than in hypertrophy of the palatine tonsils.

Selected Immunohistochemical Assessment and Clinical Examinations in the Diagnosis of Palatine Tonsil Diseases

“…For the study, we selected those cytokines that appear to be most relevant in the pathogenesis of palatine tonsil disease in the literature. Therefore, we analyzed the following: CD25, CD40, CD69, IL-1, IL-2, IL-6, IL- 8, IL-10, and IL37 [ 13 , 14 , 15 , 16 , 17 , 18 ].…”

Algorithms for the diagnosis and treatment of chronic tonsillitis

“…Хрони ческое воспаление небных миндалин, как правило, разви вается и протекает на фоне нарушений гуморального и клеточного иммунитета с угнетением неспецифических факторов естественной резистентности организма [8][9][10]. В то же время в ткани небных миндалин наблюдаются структурные изменения, которые при хроническом тонзил лите проявляются в виде перестройки сосудистого русла с постепенным замещением паренхимы соединительной тканью за счет клеточной деятельности фибробластов и образованием инкапсулированных очагов некроза с вовлечением в патологический процесс регионарных лимфатических узлов [11,12]. Структурные изменения хорошо идентифицируются при использовании спектраль ных методов диагностики, включая ультразвуковое иссле дование (УЗИ) небных миндалин, что позволяет дополнить диагностический алгоритм при хроническом тонзиллите для уточнения его формы, а также для контроля эффектив ности лечения [13].…”