Occurrence of enterotoxin genes and macrorestriction analysis of Staphylococcus aureus isolated from bovine mastitis and bulk-tank milk samples in Italy. An epidemiological study

Serraino, Andrea; Alberghini, Leonardo; Fontana, Maria Cristina; Annemüller, C.; Lämmler, Christoph; Rosmini, Roberto

doi:10.4081/ijas.2004.47

Cited by 7 publications

(4 citation statements)

References 18 publications

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“…Th e most frequently observed SE was SEA, observed in 12 samples (16.7%), followed by SED (9 samples, 12.5%) and SEC (6 samples, 8.3%). Th ese fi ndings are in agreement with Normano et al (13), Morandi et al (8), and Serraino et al (14), who reported frequent fi ndings of enterotoxins A and D in many cow dairy products. However, some of our fi ndings diff ered from those reported by other researchers in other countries.…”

Section: Sample Testedsupporting

confidence: 90%

Detection of classical enterotoxins of Staphylococcus aureus in raw sheep, goat, camel, and water buffalo milk by ELISA method

Rahimi

2011

Comp Clin Pathol

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Milk and dairy products are frequently contaminated with exterotoxigenic Staphylococcus aureus, which is oft en involved in Staphylococcal food poisoning. Th e aim of the present study is to investigate the presence of classical enterotoxins of S. aureus in raw milk that was produced in Isfahan, Iran. For this purpose, 72 milk samples were collected from the bulk milk tanks of 12 milking farms during 3 diff erent seasons (winter, spring, and summer) and tested for the presence of staphylococcal enterotoxins (SEs) using the enzyme-linked immunosorbent assay (ELISA) method. Out of 72 samples studied, 15 samples (20.8%) were positive for at least 1 SE. Of these, 12 (16.7%) were positive for SEA, 9 (12.5%) for SED, and 6 (8.3%) for SEC. None of the samples was positive for SEB or SEE. Statistical evaluation showed that there were not any signifi cant diff erences (P > 0.05) between the presence of SEs in the milk samples tested in winter, spring, and summer. Further studies should be carried out to investigate the presence of these toxins in diff erent foods and their roles in food poisoning.

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Section: Sample Testedsupporting

confidence: 90%

Detection of classical enterotoxins of Staphylococcus aureus in raw sheep, goat, camel, and water buffalo milk by ELISA method

Rahimi

2011

Comp Clin Pathol

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“…On the other hand, higher contamination rates have also been found [ 35 - 39 ]. The contamination of milk can be internally through the secretion of milk from the infected animal or externally through the infected persons (approximately, 50% of the human population carries S. aureus as commensals) or through the environment (soil, water, dust, and air) [ 40 ]. Milk and milk products are widely consumed since ancient times and its market demand is continuous worldwide [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Detection of Staphylococcus aureus enterotoxigenic strains in bovine raw milk by reversed passive latex agglutination and multiplex polymerase chain reaction

et al. 2017

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Aim:This review gives an outline of the assessment of enterotoxigenic Staphylococcus aureus tainting levels in raw milk from different sources in Egypt and characterization of enterotoxigenic strains utilizing a technique in light of PCR to identify genes coding for the production of staphylococcal enterotoxin (SE). The obtained data were compared with results from the application of the reversed passive latex.Materials and Methods:Multiplex PCR and reversed passive latex agglutination (RPLA) were used. A total of 141 samples of raw milk (cow’s milk=33, buffalo’s milk=58, and bulk tank milk=50) were investigated for S. aureus contamination and tested for enterotoxin genes presence and toxin production.Results:S. aureus was detected in 23 (16.3%) samples phenotypically and genotypically by amplification of nuc gene. The S. aureus isolates were investigated for SEs genes (sea to see) by multiplex PCR and the toxin production by these isolates was screened by RPLA. SEs genes were detected in six isolates (26.1%) molecularly; see was the most observed gene where detected in all isolates, two isolates harbored seb, and two isolates harbored sec. According to RPLA, three isolates produced SEB and SEC.Conclusion:The study revealed the widespread of S. aureus strains caring genes coding for toxins. The real significance of the presence of these strains or its toxins in raw milk and their possible impact a potential hazard for staphylococcal food poisoning by raw milk consumption. Therefore, detection of enterotoxigenic S. aureus strains in raw milk is necessary for consumer safety.

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“…aureus represents one of the most famous microorganisms found in raw milk, that milk offers a suitable medium for this bacterium growth especially when decreasing of hygienic measures as well as of cooling services occurred (16). Milk contamination could be internal through infected animals' secretion, or external due to infected persons because of the commensalism the human population and S. aureus bacteria that approximately 50% of the people are S. aureus carriers, or might cause through the environment such as water, air, soil or dust (17).…”

Section: Discussionmentioning

confidence: 99%

Isolation and molecular detection of enterotoxigenic Staphylococcus aureus from raw milk of cows

Taher¹,

Yassin²,

Abdulkareem³

2021

IJVS

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Staphylococcus aureus is a microbe associated with human's food poisoning. It is caused by isolates producing different heat stable enterotoxins which act as one of the most spread worldwide gastroenteritis causes. The goal of the current study was to assess the incidence of S. aureus (enterotoxigenic) in the raw milk samples of cows which were collected from various places of Baghdad city. Isolation and identification of S. aureus were done by conventional laboratory method and the diagnoses were confirmed by using molecular method RT-PCR to detect the specific gene of S. aureus nuc gene. In addition, we investigate the occurrence of some of Staphylococcal enterotoxins genes such as SEA, SEB, SEC, SED, and SEE. We found that 12/50 (24%) of the isolates were S. aureus and these isolates carried one or more of the enterotoxin genes such as enterotoxin type SEC 12/12 (100) followed by enterotoxin type SEA 7/12 (58.33%) and only 1/12 (8.33%) was type SED, while type SEB and SEE were negative 0%. Some of the isolates have genes that coded for two enterotoxins patterns such as SEA and SEC 7/12 (58.33%) and SEC and SED 1/12 (8.33%). Fewer of the isolates expressed only one genotype of enterotoxin gene like SEC 4/12 (33.3%). This study has proven that there was a high chance of occurrence for the enterotoxigenic S. aureus at the raw milk of cows in Baghdad city.

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Occurrence of enterotoxin genes and macrorestriction analysis of Staphylococcus aureus isolated from bovine mastitis and bulk-tank milk samples in Italy. An epidemiological study

Cited by 7 publications

References 18 publications

Detection of classical enterotoxins of Staphylococcus aureus in raw sheep, goat, camel, and water buffalo milk by ELISA method

Detection of classical enterotoxins of Staphylococcus aureus in raw sheep, goat, camel, and water buffalo milk by ELISA method

Detection of Staphylococcus aureus enterotoxigenic strains in bovine raw milk by reversed passive latex agglutination and multiplex polymerase chain reaction

Isolation and molecular detection of enterotoxigenic Staphylococcus aureus from raw milk of cows

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