Occurrence of ‘Candidatus Phytoplasma omanense’-related strains and other phytoplasmas in Sophora alopecuroides plants showing dwarfing and yellowing

Esmaeilzadeh‐Hosseini, Seyyed Alireza; Satta, Eleonora; Babaei, Ghobad; Salehi, Mohammad; Bertaccini, Assunta

doi:10.1007/s13313-020-00712-w

Cited by 5 publications

(6 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Phytoplasmas included in other 16SrIX subgroups are reported in Iran in shrubs or crops: 16SrIX‐I in Onobrychis vicifollia (Esmaeilzadeh‐Hosseini et al, 2016), 16SrIX‐D in Chrysanthemum morifolium (Bayat et al, 2013), 16SrIX‐C and 16SrIX‐H in Sophora spp. (Allahverdi et al, 2017; Esmaeilzadeh‐Hosseini et al, 2020) and 16SrIX‐C in Brassica rapa (Azadvar & Baranwal, 2010). Phytoplasmas included in this ribosomal group, therefore, have important impacts in Iran agriculture.…”

Section: Discussionmentioning

confidence: 99%

“…Total DNA was extracted from 0.3 g midrib tissue of fresh leaves using the procedure of Zhang et al (1998). Total DNA extracted from a Ziziphus jujube plant infected with a phytoplasma strain 16SrVI-A (Babaei et al, 2020) was used as positive control and a DNA sample from a healthy almond plant grown in the greenhouse was used as negative control. DNA samples were subjected to nested PCR, using primers P1/P7 (amplifying 16SrRNA gene, 16S-23S spacer region and the 5′ end of the 23S rRNA gene) (Deng & Hiruki, 1991;Schneider et al, 1995) for direct amplification.…”

Section: Introductionmentioning

confidence: 99%

“…Total DNA extracted from a Ziziphus jujube plant infected with a phytoplasma strain 16SrVI-A (Babaei et al, 2020) was used as positive control and a DNA sample from a healthy almond plant grown in the greenhouse was used as negative control. DNA samples were subjected to nested PCR, using primers P1/P7 (amplifying 16SrRNA gene, 16S-23S spacer region and the 5′ end of the 23S rRNA gene) (Deng & Hiruki, 1991;Schneider et al, 1995) for direct amplification. Subsequently, primers R16mF2/R16mR2 (amplifying approximately 1.4 kbp inside the 16SrRNA gene; Deng & Hiruki, 1991;Schneider et al, 1995) and R16F2n/R16R2 (amplifying approximately 1.2 kbp inside the 16SrRNA gene; Gundersen & Lee, 1996) were used to obtain virtual and real RFLP profiles, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…DNA samples were subjected to nested PCR, using primers P1/P7 (amplifying 16SrRNA gene, 16S-23S spacer region and the 5′ end of the 23S rRNA gene) (Deng & Hiruki, 1991;Schneider et al, 1995) for direct amplification. Subsequently, primers R16mF2/R16mR2 (amplifying approximately 1.4 kbp inside the 16SrRNA gene; Deng & Hiruki, 1991;Schneider et al, 1995) and R16F2n/R16R2 (amplifying approximately 1.2 kbp inside the 16SrRNA gene; Gundersen & Lee, 1996) were used to obtain virtual and real RFLP profiles, respectively. The PCR reaction was performed using EmeraldAmp Max HS PCR master mix (Takara, Japan), in 33 cycles in a Bio-Rad thermal cycler (Bio-Rad, USA) with denaturation for 30 s at 94°C (2 min in the first round), annealing for 30 s at 55°C and primer extension for 1 min at 72°C (10 min in the final cycle).…”

Section: Introductionmentioning

confidence: 99%

“…in India. Indian Journal of Virology, 21, 133-139. https://doi.org/10.1007/s1333 7-011-0023-6 Babaei, G., Esmaeilzadeh-Hosseini, S. A., Zandian, M.,& Bertaccini, A. (2020).…”

mentioning

confidence: 99%

See 4 more Smart Citations

Identification of a 16SrIX‐B phytoplasma strain associated with Daphne mucronata phyllody in Iran

Babaei

Esmaeilzadeh‐Hosseini

Shirmardi

et al. 2021

Forest Pathology

View full text Add to dashboard Cite

During surveys from 2017 to 2018, a phyllody disease was observed in Daphne mucronata plants located in Lordegan and Ahmadliveh areas in Chaharmahal and Bakhtiari province (west of Iran). The main symptoms were yellowing, little leaf, phyllody and flower sterility. Total DNA was extracted from midrib tissue of fresh leaves from 30 symptomatic and five asymptomatic plants. DNA samples were subjected to nested PCR, using primer pairs P1/P7 and R16F2n/R16R2. PCR amplicons of 1.8 and 1.25 kb, respectively, were obtained from all samples from symptomatic plants whilst no amplification was observed in samples from asymptomatic plants. Restriction fragment length polymorphism (RFLP) analysis of R16F2n/R16R2 amplicons using MseI,

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…in India. Indian Journal of Virology, 21, 133-139. https://doi.org/10.1007/s1333 7-011-0023-6 Babaei, G., Esmaeilzadeh-Hosseini, S. A., Zandian, M.,& Bertaccini, A. (2020).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Identification of a 16SrIX‐B phytoplasma strain associated with Daphne mucronata phyllody in Iran

Babaei

Esmaeilzadeh‐Hosseini

Shirmardi

et al. 2021

Forest Pathology

View full text Add to dashboard Cite

show abstract

Molecular diversity of phytoplasmas associated with eggplant phyllody disease in Iran

Salehi

Esmaeilzadeh‐Hosseini

Salehi

et al. 2021

Eur J Plant Pathol

View full text Add to dashboard Cite

During 2015-18, surveys were conducted in the main eggplant growing areas of Iran and in all areas phytoplasma-type symptoms were observed. A total of 350 symptomatic eggplant plants were collected and tested for the phytoplasma presence on 16S rDNA. Diversity of the detected phytoplasmas was verified by molecular analyses, dodder and graft transmission on experimental test plants. Phytoplasmas were detected in all symptomatic samples and, by using nucleotide sequence comparisons and virtual restriction fragment length polymorphism analyses of 16S rDNA, six subgroups including 16SrII-D and -V, 16SrIX-C and -I, 16SrVI-A and 16SrXII-A and molecular variants related to 16SrII-D, 16SrVI-A, 16SrIX-C subgroups were identified. Based on symptomatology in dodder and graft inoculated eggplant and periwinkle plants, the phytoplasmas enclosed in the identified subgroups were differentiable. Collectively, based on the results of the present study and considering the reported presence of phytoplasmas belonging to the same ribosomal subgroups in other crops, eggplant fields play an important role in the epidemiology of other diseases associated with these phytoplasmas in Iran.Phytoplasmas are associated with different destructive plant diseases worldwide (Bertaccini et al., 2014) and are transmitted mainly by leafhoppers, however they could be disseminated also by propagation materials and in several cases by seeds (Satta et al., 2019). Phytoplasma presence is associated with symptoms of yellowing, discoloration, witches' broom, dwarfing, virescence, and phyllody. More than 1000 plant species from different plant families are reported as affected by phytoplasmas (Bertaccini & Duduk, 2009;Lee et al., 2000) and among them, vegetables growing in the major production areas worldwide, are infected by phytoplasmas belonging to numerous ribosomal groups (Kumari et al., 2019). In particular, eggplant (Solanum melongena L.) was reported as infected with strains belonging to 16SrI in Japan, Bangladesh and India

show abstract

Diversity, distribution, and status of phytoplasma diseases in Iran

Esmaeilzadeh‐Hosseini¹,

Azadvar²,

Babaei³

et al. 2023

Diversity, Distribution, and Current Status

View full text Add to dashboard Cite

Occurrence of ‘Candidatus Phytoplasma omanense’-related strains and other phytoplasmas in Sophora alopecuroides plants showing dwarfing and yellowing

Cited by 5 publications

References 41 publications

Identification of a 16SrIX‐B phytoplasma strain associated with Daphne mucronata phyllody in Iran

Identification of a 16SrIX‐B phytoplasma strain associated with Daphne mucronata phyllody in Iran

Molecular diversity of phytoplasmas associated with eggplant phyllody disease in Iran

Diversity, distribution, and status of phytoplasma diseases in Iran

Contact Info

Product

Resources

About