Occurrence of a few organo-arsenicals in jellyfish

Abstract

Water‐soluble fractions containing arsenic compounds were extracted with chloroform‐methanol (2:1) from two kinds of jellyfish, Aurelia aurita and Carybdea rastonii. After defatting, each water‐soluble fraction was subjected to analysis by HPLC‐GFAA (column: ODS 120‐T) and HPLC‐ICP MS (column: Supelcosil LC‐SCX) for arsenicals. Arsenobetaine was detected with both analytical systems as the major arsenic compound. Besides arsenobetaine, the tetra‐methylarsonium ion and arsenocholine were also detected by HPLC‐ICP MS. The major arsenical in each jellyfish purified by ion‐exchange chromatography using Dowex 50W × 8 (H+ form) and Dowex 50W × 8 (pyridinium form) was confirmed to be arsenobetaine by thin‐layer chromatography. Copyright © 1999 John Wiley & Sons, Ltd.

Speciation of Arsenic in Different Types of Nuts by Ion Chromatography−Inductively Coupled Plasma Mass Spectrometry

“…The majority of element speciation studies have been focused on marine organisms that accumulate and metabolize arsenic (3)(4)(5)(6). Depending on the type of organism, the primary metabolites identified were arsenobetaine, arsenocholine, and arsenosugars (7)(8)(9)(10)(11). In these organisms, the total arsenic concentration levels reported ranged from 1 to 100 µg g -1 (3,5,6).…”

“…Most commonly, water or methanol/water extracts were analyzed (17,(22)(23)(24). Other solvent mixtures used for the extraction of arsenic species from marine biological samples were chloroform/methanol or chloroform/methanol/water (3,10,11,25). Different extraction conditions have also been explored, including room temperature mixing, Soxhlet extraction, supercritical fluid extraction, extraction assisted by sonication or microwave radiation (MAE), and accelerated solvent extraction (ASE) (3,14,(26)(27)(28)(29).…”