Occurrence and Topographical Mapping of Gastric <i>Helicobacter</i>‐like Organisms and Their Association with Histological Changes in Apparently Healthy Dogs and Cats

Happonen, Irmeli; Saari, Seppo; Castren, L.; Tyni, O.; Hänninen, M.-L.; Westermarck, Elias

doi:10.1111/j.1439-0442.1996.tb00457.x

Cited by 68 publications

(102 citation statements)

References 21 publications

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“…Urease testing and cytology were less sensitive and did not give any information about the species identity. Helicobacters were found less frequently in the antrum than in other regions of the stomach, a result which is in agreement with former studies (19,20).…”

Section: Discussionsupporting

confidence: 82%

Multiplex PCR Assay for Differentiation ofHelicobacter felis,H. bizzozeronii, andH. salomonis

Baele¹,

Bulck²,

Decostere³

et al. 2004

J Clin Microbiol

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Helicobacter felis, Helicobacter bizzozeronii, and Helicobacter salomonis are frequently found in the gastric mucous membrane of dogs and cats. These large spiral organisms are phylogenetically highly related to each other. Their fastidious nature makes it difficult to cultivate them in vitro, hampering traditional identification methods. We describe here a multiplex PCR test based on the tRNA intergenic spacers and on the urease gene, combined with capillary electrophoresis, that allows discrimination of these three species. In combination with previously described 16S ribosomal DNA-based primers specific for the nonculturable "Candidatus Helicobacter suis," our procedure was shown to be very useful in determining the species identity of "Helicobacter heilmannii"-like organisms observed in human stomachs and will facilitate research concerning their possible zoonotic importance.

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Section: Discussionsupporting

confidence: 82%

Multiplex PCR Assay for Differentiation ofHelicobacter felis,H. bizzozeronii, andH. salomonis

Baele¹,

Bulck²,

Decostere³

et al. 2004

J Clin Microbiol

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“…and experimental infection with H. felis. 36,79,80,91 The reasons for this difference in colonization density are unclear. Differences in the microenvironmental conditions from the different sites of the gastric mucosa may be a factor.…”

Section: Discussionmentioning

confidence: 99%

“…in the present study is similar to that reported in cats, captive exotic carnivores, and small felids naturally infected with Helicobacter. 32,36,39,48 Although the differences in histopathological findings may be related to the species of Helicobacter, factors such as the size of tissue sample examined, the age of the cat, and the presence or absence of clinical signs also must be considered. Differences in the size of the tissue samples taken from H. pylori-and H. felis-infected cats (6 mm) versus those taken from cats infected with H. heilmannii and unclassified Helicobacter spp.…”

Section: Discussionmentioning

confidence: 99%

Histological and Immunohistochemical Detection of DifferentHelicobacterSpecies in the Gastric Mucosa of Cats

et al. 2001

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Abstract. Detailed histopathological evaluation of the gastric mucosa of Helicobacter-infected cats is complicated by the difficulty of recognizing Helicobacter organisms on hematoxylin and eosin (HE)-stained sections and the ability of multiple Helicobacter species to infect cats. In this study, the presence and localization of different species of Helicobacter in the stomachs of cats was investigated using silver staining and immunohistochemistry. Five groups containing 5 cats each were established (group 1: urease negative and Helicobacter free; groups 2, 3, 4, and 5: urease positive and infected with Helicobacter heilmannii, unclassified Helicobacter spp., Helicobacter felis, and Helicobacter pylori, respectively). Gastric samples were evaluated by HE and silver staining and by immunohistochemistry with 3 different anti-Helicobacter primary antibodies. Helicobacter were detected by Steiner stain in all infected cats at the mucosal surface, in the lumen of gastric glands, and in the cytoplasm of parietal cells. In silver-stained sections, H. pylori was easily differentiated from H. felis, H. heilmannii, and unclassified Helicobacter spp., which were larger and more tightly coiled. No organisms were seen in uninfected cats. Helicobacter antigen paralleled the distribution of organisms observed in Steinerstained sections for 2 of the 3 primary antibodies tested. The antisera were not able to discriminate between the different Helicobacter species examined. A small amount of Helicobacter antigen was present in the lamina propria of 3 H. pylori-, 3 H. felis-, and 1 H. heilmannii-infected cat. Minimal mononuclear inflammation was present in uninfected cats and in those infected with unclassified Helicobacter spp. and H. heilmannii cats. In H. felis-infected cats, lymphoid follicular hyperplasia with mild pangastric mononuclear inflammation and eosinophilic infiltrates were present. The H. pylori-infected cats had severe lymphoid follicular hyperplasia and mild to moderate mononuclear inflammation accompanied by the presence of neutrophils and eosinophils. These findings indicate that Steiner staining and immunohistochemistry are useful for detecting Helicobacter infections, particularly when different Helicobacter species can be present. Monoclonal antibodies specific for the different Helicobacter species could be important diagnostic aids. There appear to be differences in the severity of gastritis in cats infected with different Helicobacter species.Gastric spiral-shaped bacteria of the genus Helicobacter have been described in many animal species as well as in humans. 24,81 Although their presence has been documented since the end of the 19th century, it is only since the discovery of Helicobacter pylori in humans by Warren and Marshall in 1983 that much interest has been expressed in gastric Helicobacter in animals. 5,74,77,87 Helicobacter pylori infection in humans has been associated with chronic active gastritis, peptic ulcer, gastric mucosa-associated lymphoid tissue lymphoma, and adenocarcinoma. 10,20,30...

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“…The high incidence of Helicobacter organisms in dogs' stomach confirmed by different studies [2,4,7,13,[17][18][19] . In spite of confirmation of H. pylori as a main cause SHABESTARI ASL, AMANIZAD, NEJAD PARTOVI, JALILI, MOHAMMAD NEZHAD, SOROUSH, BARZEGARI, BABAZADEH of human chronic gastritis and gastric malignancy, the exact role of gastric helicobacters in dogs has not been established yet [2,7,8] .…”

Section: Discussionmentioning

confidence: 75%

“…[1,8,11,12] , but the exact role of these fastidious bacteria is not confirmed yet [2,7,8] . Until now, different Helicobacter species have been isolated from canine stomachs [2,4,[13][14][15] ; but it is not known whether these species are representative of all canine gastric helicobacters or which of them are most common [8,14] . Different studies have reported dissimilar ranges of contamination [3][4][5][6]16] and some of these reports were showed high prevalence (over 90%) of contaminations [17][18][19] .…”

Section: öZetmentioning

confidence: 99%

Assessment of Gastric Helicobacter spp. in Fresh Gastric Samples of Naturally Infected Dogs by Scanning Electron Microscopy

Asl¹,

Amanizad²,

Partovi³

et al. 2014

Kafkas Univ Vet Fak Derg

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SummaryDifferent species of gastric Helicobacter-Like Organisms (GHLO) reported from dogs' stomach. The aim of present study was to morphological evaluation of gastric Helicobacter spp. in fresh gastric samples of naturally infected dogs. Thirty two gastric samples of the stray dog were taken at necropsy. The specimens were used for rapid urease test, light microscopy, scanning electron microscopy (SEM) and polymerase chain reaction (PCR). Light microscopy examination confirmed the presence of GHLO in 90.5% of stray dogs. 87.5% and 94% of gastric samples were positive in rapid urease test and PCR, respectively. Four distinguishable Helicobacter organisms were confirmed by SEM. Three strains of these organisms were inditified as H. felis, candidatus H. heilmanii and H. bizzozeronii because of their apparent morphological differences and PCR results. The last strain of these bacteria was not distinguishable with routine studies. The large-scale studies with fast and simple recognition methods are recommended to confirm the different types of canine gastric Helicobacter. The results of present study showed further investigation in canine GHLO is required because new species of Helicobacter reported.

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Occurrence and Topographical Mapping of Gastric Helicobacter‐like Organisms and Their Association with Histological Changes in Apparently Healthy Dogs and Cats

Cited by 68 publications

References 21 publications

Multiplex PCR Assay for Differentiation ofHelicobacter felis,H. bizzozeronii, andH. salomonis

Multiplex PCR Assay for Differentiation ofHelicobacter felis,H. bizzozeronii, andH. salomonis

Histological and Immunohistochemical Detection of DifferentHelicobacterSpecies in the Gastric Mucosa of Cats

Assessment of Gastric Helicobacter spp. in Fresh Gastric Samples of Naturally Infected Dogs by Scanning Electron Microscopy

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