Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia

Marković, Sanja; Stanković, Slaviša; Jelušić, Aleksandra; Iličić, Renata; Kosovac, Andrea; Poštić, Dobrivoj; Popović, Tatjana

doi:10.1094/pdis-05-20-1076-re

Cited by 18 publications

(22 citation statements)

References 40 publications

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“…Scotch Cara and may indicate the susceptibility of the reported cultivar for soft rot and/ or blackleg infection. Furthermore, the results of this current study highlight a concordance to results were reported by Marković et al (2021) stated that two types of tissue decomposition could be notified for these diseases: the first group of isolates (Like E3, E4 and E7 for Spunta variety, and E4 and E7 for Desiree variety) produced cream-colored rotting tissue with dark brown margins in the zone between the healthy and decomposed tissue, and the second group (Such as isolates E5 for Spunta variety, and E3 and E5 for Desiree variety) devastated the tissue causing cream-colored rotting but with no visible margins.…”

Section: Resultssupporting

confidence: 93%

RAPID SCREENING OF PHYTOPATHOGENIC Erwinia sp. OF TWO POTATO VARIETIES (SPUNTA AND DESIREE) FROM ALGERIAN AGRICULTURAL FIELDS

Mokrani

Nabti

2021

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

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Rapid screening of phytopathogenic Erwinia sp. of two potato varieties (Spunta and Desiree) from Algerian agricultural fileds. Isolation, phenotypic identification and in vitro phytopathogenicity screening of Erwinia sp. from agricultural field of two potato varieties (Spunta and Desiree) in Algeria. The current study aims to isolate, identify and screen phytopathogenic isolates of Erwinia sp. causing potato diseases. The techniques presented in this study for isolation and characterization of phytopathogenic Erwinia sp. are conventional methods that are used in this field of research. Seven phytopathogenic bacteria were recovered from potato tubers of two varieties (Spunta and Desiree). The phenotypic identification allowed characterizing typical colonies of Erwinia sp. on two semi-selective media: King’s B and TCC media. Erwinia sp. formed characteristic colonies on King’s B medium that were round, convex and representing creamy color. While, Erwinia sp. also developed specific colonies on TCC medium which were pale purple, circular, convex, even bulging; smooth and mucous. In vitro phytopathogenicity test on potato slices lead to screen the phytopathogenic isolate E5 characterized by highest rotten tissue zone of (2.33 ± 0.29 cm) and (2.33 ± 0.58 cm) toward Spunta and Desiree varieties, respectively. Followed, by isolate E4 characterized by rotten tissue zone of (1.83 ± 0.58 cm) and (2.17 ± 0.29 cm) toward Spunta and Desiree varieties, respectively; compared to their corresponding uninfected controls. The RTZ (Rotten tissue zone) evidently is proportional to the specific pathogenicity of Erwinia sp. isolates and the characteristic sensitivity of various varieties (Spunta and Desiree). Thus, make determining RTZ a rapid screening technique for the selection of the highest phytopathogenic isolates. This investigation provides valuable information for rapid screening (infected potato tuber) and characterization (isolation using semi-selective media) of pathogenic Erwinia sp. engendering potato disease compared to existing methods like infection of leaves or plants; and phytopathogenic Erwinia sp. identification through PCR amplification or in situ hybridation.

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Section: Resultssupporting

confidence: 93%

RAPID SCREENING OF PHYTOPATHOGENIC Erwinia sp. OF TWO POTATO VARIETIES (SPUNTA AND DESIREE) FROM ALGERIAN AGRICULTURAL FIELDS

Mokrani

Nabti

2021

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

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“…Using traditional isolation and molecular characterization in the same eld in Northern Serbia during 2018, combined infection of P. carotovorum subsp. brasiliensis and D. dianthicola was determined (Marković et al 2020) while the Dickeya spp. was found at the limit of detection in metabarcoding samples.…”

Section: Discussionmentioning

confidence: 99%

“…According to the whole dataset with singletons excluded, the Dickeya genus was not found, although bacteria belonging to the D. dianthicola were isolated and identi ed from the same eld (Marković et al 2020).…”

Section: Bacterial Community Compositionmentioning

confidence: 99%

The Potential use of Bacterial Diversity Analysis of Soft Rotted Potato Tubers and Their Geocaulospheres

Marković¹,

Popović²,

Berić³

et al. 2021

Preprint

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The soft rot caused by Pectobacterium and Dickeya spp. is among the most important potato disease, responsible for outbreaks worldwide. In 2018, potato tubers (cultivar Lady Claire) with and without visible soft rot symptom, together with their geocaulospheres were sampled from the field in Bačka region (Serbia). The 16S rRNA Next Generation Sequencing (NGS) of tubers with and without soft rot symptom and their corresponding soils was performed to detect differences in microbial diversity and, using this data to predict causal agent(s) of disease and which samples are potentially best for isolating biocontrol strains. The ubiquitous soil bacteria from the phyla Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes were dominant in all samples. The sequences identified as Pectobacterium aroidearum, P. carotovorum, and P. polaris were present in all tested samples and it can be hypothesized that they caused soft rot. The K3 sample showed the presence of genera with potential antimicrobial activity (Pseudomonas, Arthrobacter, Chryseobacterium, Bacillus, and Exiguobacterium). This result shows that diversity analysis could be used for checking for the presence of potential antagonistic bacteria at infected sites. Also, following the presence or absence of particular taxa could point out a capacity of soil to endure a growth season without an outbreak of soft or to maintain it without significant losses. This is a new approach to interpreting the results of the diversity of bacterial communities of tubers and can be useful for screening the health status of the soil.

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“…Multilocus sequence analysis (MLSA) was performed with a total of 36 randomly selected isolates, with three per year of isolation, location, and potato cultivar (Table 1). Total DNA was extracted using the modified CTAB protocol described by Marković et al (2021).…”

Section: Methodsmentioning

confidence: 99%

Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers

Marković¹,

Stanković

Iličić

et al. 2021

Plant Pathology

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Since 2011, the outbreaks of brown rot caused by Ralstonia solanacearum race 3, biovar 2, phylotype IIB‐1 (R3/B2/PIIB‐1) have significantly compromised potato production in Serbia. During 6 years of monitoring (2013–2018) among 3,524 potato tuber samples, 344 were found positive for brown rot disease. R. solanacearum R3/B2/PIIB‐1 was isolated from seven cultivars among 12 monitored, and in five localities among 17 monitored. Cultivar Lady Claire was found to have the highest disease frequency (31.98%). A total of 78 isolates were identified by R. solanacearum‐specific primer pairs (PS‐1/PS‐2 and OLI‐1/Y‐2), as well as the following tests: restriction fragment length polymorphism analysis, biovar determination, immunofluorescence, biochemical analysis, and pathogenicity. The genetic composition of 36 selected isolates assessed using multilocus sequence analysis with seven genes (adk, gapA, gdhA, gyrB, ppsA, hrpB, and fliC) showed that all isolates originating from Serbian potato were homogeneous. By using the TCS algorithm of concatenated sequences to get insight into the phylogeography of isolates and other R. solanacearum strains deposited in the NCBI database, we showed that their origin is undetermined. Peroxidase (POD) activity was measured in brown rotted potato tubers. A positive correlation was found between POD activity and disease severity rated on the analysed tubers. In general, POD activity increased by 2–22 times in vascular necrotic tissues compared to non‐necrotic ones, and depended on disease severity but not on cultivar. Native polyacrylamide gel electrophoresis analysis of POD profiles resulted in a total of 10 distinct POD isoforms, of which PODs 3–5 were highly intensified in response to R. solanacearum.

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Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia

Cited by 18 publications

References 40 publications

RAPID SCREENING OF PHYTOPATHOGENIC Erwinia sp. OF TWO POTATO VARIETIES (SPUNTA AND DESIREE) FROM ALGERIAN AGRICULTURAL FIELDS

RAPID SCREENING OF PHYTOPATHOGENIC Erwinia sp. OF TWO POTATO VARIETIES (SPUNTA AND DESIREE) FROM ALGERIAN AGRICULTURAL FIELDS

The Potential use of Bacterial Diversity Analysis of Soft Rotted Potato Tubers and Their Geocaulospheres

Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers

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