Occurrence and Distribution of Multiple Antibiotic Resistance Bacteria of Public Health Significance in Backwaters and Aquaculture Farm

Pokhrel, Hemanta; Baishya, Sangipran; Phukan, Bipul; Pillai, Devika; Rather, Mohd Ashraf

doi:10.20546/ijcmas.2018.702.121

Cited by 15 publications

(9 citation statements)

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“…MAR index of more than 0.2 is evidence of highrisk source contamination (Krumperman, 1983) . The origin of the isolates from highly contaminated areas executes a much higher risk of spreading MAR to the aquatic environment, subsequently will affect aquaculture production (Pokhrel et al, 2018) . The recommendation of decrease antibiotics usage in aquacultures besides raising aquaculture immunity would inhibit the irrational spreading of drug-resistant pathogens.…”

Section: Discussionmentioning

confidence: 99%

Virulent and Multiple Antimicrobial Resistance Aeromonas hydrophila Isolated from Diseased Nile Tilapia Fish (Oreochromis niloticus) in Egypt with Sequencing of Some Virulence-Associated Genes

Saleh¹,

Elkenany

Younis

2021

Biocontrol Sci.

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Aeromonas hydrophila is a major waterborne pathogen, which induces various diseases in freshwater fish with the capability for zoonotic potential. This study was applied to investigate the prevalence of A. hydrophila in diseased Nile tilapia fish, genetic characterization of the virulence encoding genes (act, aerA, alt, and ast genes) , and antibiotic susceptibility. Out of the 500 diseased Nile tilapia fish samples, 70% (350/500) Aeromonas species were isolated. From which 53.4% (187/350) of Aeromonas hydrophila strains were identified. A. hydrophila was detected in kidneys, followed by liver, spleen, intestine, and gills. The results of virulotyping displayed the presence of act, and aerA genes in a high percentage of 40%, followed by alt gene (30%) , but ast gene was not detected (0%) in A. hydrophila strains. Based on DNA sequence analysis of three virulence associated-genes (act, aerA, and alt genes) , the phylogenetic tree showed the genetic relationship with related species. Finally, the antibiotic susceptibility tests revealed high resistance toward chloramphenicol (67.4%) , followed by amikacin (51.9%) and gentamicin (47.1%) , whereas a high sensitivity was exhibited toward meropenem (90.9%) , followed by ciprofloxacin (84.2%) , amoxicillin-clavulanic acid (73.3%) and trimethoprimsulfamethoxazole (64.2%) . The multidrug-resistant A. hydrophila strains were observed in 69.0% of strains with six resistance patterns.

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Section: Discussionmentioning

confidence: 99%

Virulent and Multiple Antimicrobial Resistance Aeromonas hydrophila Isolated from Diseased Nile Tilapia Fish (Oreochromis niloticus) in Egypt with Sequencing of Some Virulence-Associated Genes

Saleh¹,

Elkenany

Younis

2021

Biocontrol Sci.

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“…The mounting use of antibiotics, not only in health care but also in agriculture and animal husbandry contribute to an emergent problem of antibiotic resistant bacteria (Dhanorkar and Tambekar, 2004). Pokhrel et al (2018) reported, among the isolated environmental bacteria, 3-antibiotic resistance, 4 to 10-antibiotic resistances and more than 10-antibiotic resistance in 6.1%, 44.89% and 48.97% isolates, respectively. The fecal as well as soil isolates of Klebsiella, Citrobacter, Shigella and Staphylococcus, showed resistance to 6 -10 antibiotics tested, and the MAR indices for the isolates ranged 6 -10 (Ayandele et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

Heavy metal tolerance in association with plasmid mediated multiple antibiotic resistances among clinical bacterial isolates

Das¹,

Mandal²,

Mandal³

2018

Biosci. Biotech. Res. Comm

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The heavy metal tolerance in association with plasmid mediated antibiotic resistance among bacteria has been reported around the globe. This communication conducted an experiment to explore the coexistence of antibiotic resistance and heavy metal tolerance in clinical bacteria and the involvement of R-plasmid in such phenomenon. By disc diffusion method, 6 clinical bacteria: Escherichia coli (n=3), Pseudomonas aeruginosa (n=2) and Proteus mirabilis (n=1), utilized in the study, displayed resistance to multiple antibiotics with MAR (multiple antibiotic resistance) indices 0.15-0.77; such bacterial isolates showed tolerance to Hg 2+ , Cd 2+ , Cr 6+ and Cu 2+ at 3-37.5 μg/ml, 75-800 μg/ml, 100-400 μg/ ml and 600-900 μg/ml, respectively. The SDS treatment induced the test bacteria to mislay their resistance property (following susceptibility test) with a parallel loss of single plasmid (following agarose gel electrophoretic analysis) contained in them. This study confi rms the antibiotic co-resistance with heavy metal tolerance among human pathogenic bacteria, and underlines the regular vigilance of bacterial R-plasmid in order to combat the multiple antibiotic resistances of such bacteria as well as the infection caused by them.

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“…An MAR index above 0.2 is a parameter used to reveal the spread of bacterial resistance in certain population. An MAR index more than 0.2 reveals that the strains of such bacteria originated from a habitat in which different antibiotics are used and the abuse of antibiotics in aquatic and environmental systems [50,51]. A high incidence of MAR is permitted by genetic exchange between MAR pathogens and other bacteria [38].…”

Section: Discussionmentioning

confidence: 99%

“…The use of antimicrobials in aquaculture, could impose an impact on the development of resistance in human health known as a direct spread of resistance from aquatic environments to human. Similarly, increasing microbial resistance problems could spread from country to another, for instant, the export/import of foods like fish and fishery products [51].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Antibiogram of Vibrio parahaemolyticus and Aeromonas hydrophila in the Flesh of Nile Tilapia, with Special Reference to Their Virulence Genes Detected Using Multiplex PCR Technique

Zaher

Nofal²,

Hendam

et al. 2021

Antibiotics

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Vibrio parahaemolyticus and Aeromonas hydrophila are major public health problems and the main cause of bacterial disease in Nile tilapia (Oreochromis niloticus). This study was conducted to determine the prevalence, antibiotic resistance and some virulence genes of both V. parahaemolyticus and A. hydrophila isolates from Nile tilapia. From Manzala Farm at Dakahlia governorate, 250 freshwater fish samples were collected. The confirmed bacterial isolates from the examined Nile tilapia samples in the study were 24.8% (62/250) for V. parahaemolyticus and 19.2% (48/250) for A. hydrophila. multiplex PCR, revealing that the tlh gene was found in 46.7% (29/62) of V. parahaemolyticus isolates, while the tdh and trh virulence genes were found in 17.2% (5/29). Meanwhile, 39.5% (19/48) of A. hydrophila isolates had the 16s rRNA gene and 10.5% (2/19) had the aerA and ahh1 virulence genes. The Multiple Antibiotic Resistance indices of V. parahaemolyticus and A. hydrophila were 0.587 and 0.586, respectively. In conclusion, alternative non-antibiotic control strategies for bacterial infections in farmed fish should be promoted to avoid multidrug-resistant bacteria. Therefore, it is suggested that farmers should be skilled in basic fish health control and that molecular detection methods are more rapid and cost-effective than bacteriological methods.

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Occurrence and Distribution of Multiple Antibiotic Resistance Bacteria of Public Health Significance in Backwaters and Aquaculture Farm

Cited by 15 publications

References 0 publications

Virulent and Multiple Antimicrobial Resistance <i>Aeromonas hydrophila</i> Isolated from Diseased Nile Tilapia Fish (<i>Oreochromis niloticus</i>) in Egypt with Sequencing of Some Virulence-Associated Genes

Virulent and Multiple Antimicrobial Resistance <i>Aeromonas hydrophila</i> Isolated from Diseased Nile Tilapia Fish (<i>Oreochromis niloticus</i>) in Egypt with Sequencing of Some Virulence-Associated Genes

Heavy metal tolerance in association with plasmid mediated multiple antibiotic resistances among clinical bacterial isolates

Prevalence and Antibiogram of Vibrio parahaemolyticus and Aeromonas hydrophila in the Flesh of Nile Tilapia, with Special Reference to Their Virulence Genes Detected Using Multiplex PCR Technique

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