Occurrence and clonal relatedness of sec/tst-gene positive Staphylococcus aureus isolates of quartermilk samples of cows suffering from mastitis

Zschöck, M.; Risse, Kathrin; Sommerhäuser, Jürgen

doi:10.1111/j.1472-765x.2004.01519.x

Cited by 22 publications

(16 citation statements)

References 20 publications

(32 reference statements)

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“…Strains of S. aureus have been observed to produce numerous putative virulent extracellular factors (protein A, clumping factor, fibronectin, fibrinogen, collagen and pseudo capsule ligand binding proteins etc. ), enzymes (hemolysins, coagulase, nucleases, and collagenase), and enterotoxins, and exfoliative toxins (Sutra and Poutrel 1994;Zschöck et al 2004;Reinoso et al 2008). The adhesin genes have been attributed to express different proteins that are imperatively involved in the adhesion, invasion, and infection of S. aureus (Palma et al 1999;Zecconi et al 2005).…”

Section: Introductionmentioning

confidence: 98%

Prevalence of adhesin and toxin genes among isolates of Staphylococcus aureus obtained from mastitic cattle

Kumar

Yadav

Anand

et al. 2010

World J Microbiol Biotechnol

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Staphylococcus aureus is recognized as a major etiological agent of the most important udder disease; mastitis. The virulence potential of S. aureus isolates is attributed to a combination of extracellular factors and invasive properties that are controlled at the genomic level. In this study molecular variations were studied among the 128 S. aureus isolates obtained from the mastitic crossbred cattle. The polymorphic patterns of protein A, coagulase, and IG genes were studied in the isolates using the PCR and PCR-RFLP methods. In addition, presence of other virulence genes responsible for the expression of adhesins and toxins was also screened. A considerable genetic variation was observed in amplified fragment of SPA, IG and CLF genes. The PCR-RFLP of COA gene yielded five different patterns. Occurrence of EBP, ENO and FNBA was found to be common in both high and low virulence isolates. However, the prevalence of FNBB and toxin genes was higher in clinical than subclinical isolates. The distribution proportions of adhesin genes EBP, FIB, FNBB, CNA, BBP, MAP CAP5, and CAP8 were 60.2, 59.4, 21.9, 6.3, 5.5, 80.5, 56.3, and 22.7%; for AGR I, II, III, and IV were 44.5, 32.8, 12.5, and 10.2%; and for toxins genes HLB, SEB, SEC, SED, SEE, SEG, and SEI were 82.0, 3.1, 5.5, 3.9, 0.8, 16.4, and 55.5%, respectively. The proportions of FNBB, EBP, FIB, HLB, BBP, SEG, and SEI genes were observed more in clinical cases. The significant genetic variations in SPA, COA, IG, and CLF genes were useful to differentiate the isolates that might be valuable for mastitis reduction programme.

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Section: Introductionmentioning

confidence: 98%

Prevalence of adhesin and toxin genes among isolates of Staphylococcus aureus obtained from mastitic cattle

Kumar

Yadav

Anand

et al. 2010

World J Microbiol Biotechnol

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“…In studies in which the production of staphylococcal virulence factors has been detected, most S. aureus isolates originating from bovine mastitis specimens have been shown to produce alpha-and beta-hemolysin and leukocidins or carry the respective genes (1,34). Some superantigen determinants in particular, like sec and tst, have been common and typical for certain genetic lineages (9,51). The genes seg to seo, which belong to the enterotoxigenic cluster egc, have been found frequently in S. aureus isolates from bovine mastitis specimens (9,10,40).…”

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confidence: 99%

Molecular Types and Genetic Profiles of Staphylococcus aureus Strains Isolated from Bovine Intramammary Infections and Extramammary Sites

et al. 2008

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Staphylococcus aureus isolates collected from sites of intramammary infection during a 10-month period and from extramammary sites (dairy cow teat skin, teat canals, and skin lesions; milking liners; and hands and nostrils of milking personnel) at two separately managed Finnish dairy herd establishments were analyzed to study the sources and reservoirs of bovine S. aureus intramammary infection. Selected isolates were subjected to pulsed-field gel electrophoresis (PFGE) typing and PCR analysis for genes encoding hemolysins (hla to hlg), leukocidins (lukED and lukM), superantigens (sea, sec, sed, seg to seo, seu, and tst), adhesins (fnbA and fnbB), and penicillin and methicillin resistance (blaZ and mecA). S. aureus was found throughout the herds in 94% of the cows. Nine PFGE types were found, with the herds each having their own predominant type and sharing one type. The degree of diversity of PFGE types in herd II, which integrated foreign heifers, was higher than that in herd I. For both herds, the majority of the PFGE-typed isolates both from milk and from extramammary sites represented the predominant PFGE types. In isolates from herd I, the most prevalent genes were hla-hlg, lukED, and fnbA; in those from herd II, they were hla, hld, hlg, lukED, and fnbA. The other genes were pulsotype linked within the herds. The predominant PFGE types carried both fnbA and fnbB; only fnbA was detected in the other PFGE types. No connection between specific virulence genes and the origins of isolates was found. The results suggest that for the two herds, most S. aureus isolates from extramammary sites were indistinguishable from the isolates infecting the mammary gland and that those sites can thus act as origins and reservoirs of intramammary infections. However, contamination in the opposite direction cannot be excluded.

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“…tst gene was detected together with sec gene in 107 (97.3%) isolates in this study. A positive correlation between sec-tst genes has been reported in previous studies 11,12 . Co-existence of these genes could be explained by presence of sec gene on a pathogenicity island together with tst 13 .…”

Section: Discussionmentioning

confidence: 67%

Koyunlarda Gangrenli Mastitislerden İzole Edilen Staphylococcus aureus Suşlarının Toksin Genlerinin Araştırılması

Tel¹,

Aslantaş²,

Keskin³

et al. 2009

Kafkas Univ Vet Fak Derg

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SummaryIn this study, it was aimed to determine staphylococcal enterotoxin (SE) genes, toxic shock syndrome toxin (TSST) gene and exfoliative toxin (ET) genes in 110 Staphylococcus aureus strains from gangrenous mastitis cases in seven ewe flocks in Sanliurfa, Turkey. Among investigated isolates, only sec and tst toxin genes were detected. The results of this study showed that the rates of enterotoxin production were high (100%) in S. aureus strains isolated from ovine gangrenous mastitis. Moreover, it was demonstrated that S. aureus causing gangrenous mastitis harboured sec and tst genes suggesting that these genes may have a role in ovine mastitis pathogenesis. Keywords: Gangrenous mastitis, Ewe, Staphylococcus aureus, Toxin genes Koyunlarda Gangrenli Mastitislerden İzole Edilen Staphylococcus aureus Suşlarının Toksin Genlerinin Araştırılması ÖzetBu çalışmada, Şanlıurfa yöresinde, yedi koyun sürüsündeki gangrenli mastitis vakalarından izole edilen 110 Staphylococcus aureus suşlarında stafilakokal enterotoksin, toksik şok sendrom toksin ve eksfoliatif toksin genleri bakımından araştırılması amaçlandı. İncelenen suşlarda yalnızca sec ve tst toksin genleri saptandı. Bu çalışmanın sonucunda koyunlarda gangrenöz mastitislerden izole edilen S. aureus suşlarında enterotoksin üretiminin yüksek (%100) olduğu, izole edilen S. aureus suşlarının sec ve tst genlerini içerdiği ve bu genlerinde koyunlarda mastitisin patogenezisinde rol oynayabileceği kanısına varıldı.

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Occurrence and clonal relatedness of sec/tst-gene positive Staphylococcus aureus isolates of quartermilk samples of cows suffering from mastitis

Cited by 22 publications

References 20 publications

Prevalence of adhesin and toxin genes among isolates of Staphylococcus aureus obtained from mastitic cattle

Prevalence of adhesin and toxin genes among isolates of Staphylococcus aureus obtained from mastitic cattle

Molecular Types and Genetic Profiles of Staphylococcus aureus Strains Isolated from Bovine Intramammary Infections and Extramammary Sites

Koyunlarda Gangrenli Mastitislerden İzole Edilen Staphylococcus aureus Suşlarının Toksin Genlerinin Araştırılması

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