Occlusion phenomenon of redox probe by protein as a way of voltammetric detection of non-electroactive C-reactive protein

Kowalczyk, Agata; Sęk, Jakub P.; Kasprzak, Artur; Popławska, Magdalena; Grudziński, Ireneusz P.; Nowicka, Anna M.

doi:10.1016/j.bios.2018.06.019

Cited by 24 publications

(11 citation statements)

References 50 publications

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“…These sensitivities are much lower than the ones obtained in the other systems addressed in this study, which points out the lower binding affinities of the probes used in this immunoassay. However, despite these low SNR values, this sensitivity is in the concentration range of CRP in healthy humans plasma (between 3 and 10 µg mL -1 ), which increases dramatically under cardiovascular diseases, inflammations, and infections (Kowalczyk et al, 2018;Uddin et al, 2018).…”

Section: Immunosensingmentioning

confidence: 96%

Disk-based one-dimensional photonic crystal slabs for label-free immunosensing

Sancho-Fornes

Avella-Oliver

Carrascosa

et al. 2019

Biosensors and Bioelectronics

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One-dimensional photonic crystal slabs are periodic optical nanostructures that produce guided-mode resonance. They couple part of the incident light into the waveguide generating bandgaps in the transmittance spectrum, whose position is sensitive to refractive index variations on their surface. In this study, we present one-dimensional photonic crystal slab biosensors based on the internal nanogrooved structure of Blu-ray disks for label-free immunosensing. We demonstrated that this polycarbonate structure coated with a critical thickness of TiO2 generates guided-mode resonance. Its optical behavior was established comparing it with other compact disk structures. The results were theoretically calculated and experimentally demonstrated, all them being in agreement. The bioanalytical performance of these photonic crystals was experimentally demonstrated in a model assay to quantify IgGs as well as in two immunoassays to determine the biomarkers C-reactive protein and lactate dehydrogenase (detection limits of 0.1, 87, and 13 nM, respectively). The results are promising towards the development of new low-cost, portable, and label-free optical biosensors that join these photonic crystals with dedicated bioanalytical scanners based on compact disk drives.

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Section: Immunosensingmentioning

confidence: 96%

Disk-based one-dimensional photonic crystal slabs for label-free immunosensing

Sancho-Fornes

Avella-Oliver

Carrascosa

et al. 2019

Biosensors and Bioelectronics

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“…However, it is difficult to immobilize/adsorb Fc onto the surface of an electrode [44]. Branching conjugation of Fc with polymer enables the circumvention of this drawback [44][45][46][47][48][49]. For instance, Li et al synthesized a water soluble Fc-terminated hyperbranched polyurethane (HPU-Fc), which was used for the fabrication of a non-enzymatic glucose sensor through an electrodeposition process [47].…”

Section: Branched Polymersmentioning

confidence: 99%

The Bioanalytical and Biomedical Applications of Polymer Modified Substrates

Sun

2022

Polymers

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Polymers with different structures and morphology have been extensively used to construct functionalized surfaces for a wide range of applications because the physicochemical properties of polymers can be finely adjusted by their molecular weights, polydispersity and configurations, as well as the chemical structures and natures of monomers. In particular, the specific functions of polymers can be easily achieved at post-synthesis by the attachment of different kinds of active molecules such as recognition ligand, peptides, aptamers and antibodies. In this review, the recent advances in the bioanalytical and biomedical applications of polymer modified substrates were summarized with subsections on functionalization using branched polymers, polymer brushes and polymer hydrogels. The review focuses on their applications as biosensors with excellent analytical performance and/or as nonfouling surfaces with efficient antibacterial activity. Finally, we discuss the perspectives and future directions of polymer modified substrates in the development of biodevices for the diagnosis, treatment and prevention of diseases.

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“…EIS-based immunosensors are nondestructive and require no labeling of the antibody for signal generation ( Repo et al, 2002 ; Chowdhury et al, 2017 ). Electrochemical methods are based on the interaction between the antibody and the antigen or aptamer and protein interactions ( Wang et al, 2017 ; Kowalczyk et al, 2018 ). The results are direct in that the affinity interactions between the antibody and antigen can be directly detected using the changes in charge transfer resistance (R CT ) ( Rodriguez et al, 2005 ; Bogomolova et al, 2009 ).…”

Section: Introductionmentioning

confidence: 99%

Oriented Antibody Covalent Immobilization for Label-Free Impedimetric Detection of C-Reactive Protein via Direct and Sandwich Immunoassays

Adesina

Mashazi

2021

Front. Chem.

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The detection and monitoring of biological markers as disease indicators in a simple manner is a subject of international interest. In this work, we report two simple and sensitive label-free impedimetric immunoassays for the detection of C-reactive protein (CRP). The gold electrode modified with boronic acid–terminated self-assembled monolayers afforded oriented immobilization of capture glycosylated antibody (antihuman CRP monoclonal antibody, mAb). This antibody-modified surface was able to capture human CRP protein, and the impedance signal showed linear dependence with CRP concentration. We confirmed the immobilization of anti-CRP mAb using surface sensitive X-ray photoelectron spectroscopy (XPS) and electrochemical impedance. The oriented covalent immobilization of mAb was achieved using glycosylated Fc (fragment, crystallizable) region specific to boronic acid. The direct immunoassay exhibited a linear curve for concentration range up to 100 ng ml−1. The limit of detection (LoD) of 2.9 ng ml−1, limit of quantification (LoQ) of 9.66 ng ml−1, and sensitivity of 0.585 kΩ ng−1 ml cm−2 were obtained. The sandwich immunoassay was carried out by capturing polyclonal anti-CRP antibody (pAb) onto the CRP antigen immunoreaction. The impedance signal after pAb capture also showed linear dependence with CRP antigen concentration and acted as a CRP antigen detection signal amplifier. The detection of the CRP antigen using sandwich pAb immunoassay improved LoD to 1.2 ng ml−1, LoQ to 3.97 ng ml−1, and enhanced the sensitivity to 0.885 kΩ ng−1 ml cm−2. The real sample analysis, using newborn calf serum, showed excellent selectivity and % recovery for the human CRP ranging from 91.2 to 96.5%. The method was reproducible to 4.5% for direct immunoassay and 2.3% for sandwich immunoassay.

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Occlusion phenomenon of redox probe by protein as a way of voltammetric detection of non-electroactive C-reactive protein

Cited by 24 publications

References 50 publications

Disk-based one-dimensional photonic crystal slabs for label-free immunosensing

Disk-based one-dimensional photonic crystal slabs for label-free immunosensing

The Bioanalytical and Biomedical Applications of Polymer Modified Substrates

Oriented Antibody Covalent Immobilization for Label-Free Impedimetric Detection of C-Reactive Protein via Direct and Sandwich Immunoassays

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