Obtenção de híbridos somáticos de limão 'Cravo' e tangerina 'Cleópatra'

Latado, Rodrigo Rocha; Derbyshire, Maria Teresa Vitral de Carvalho; Tsai, Siu Mui; Neto, Augusto Tulmann

doi:10.1590/s0100-204x2002001200009

Cited by 5 publications

(3 citation statements)

References 15 publications

(12 reference statements)

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“…In Brazil, citrus protoplast fusion approaches have been developed since 1995, leading to the production of somatic hybrids for rootstock improvement aiming at the control of important diseases, such as blight and citrus tristeza virus (CTV) (Mendes-da-Glória et al, 2000;Mendes et al, 2001;Latado et al, 2002;Costa et al, 2003).…”

Section: Hibridação Somática Entre Citrus Sinensis (L) Osbeck E C Gmentioning

confidence: 99%

Somatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) Osbeck

Calixto

Filho

Mendes

et al. 2004

Pesq. agropec. bras.

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This work had as objective to produce citrus somatic hybrids between sweet oranges and pummelos. After chemical fusion of sweet orange embryogenic protoplasts with pummelo mesophyll-derived protoplasts, plants were regenerated by somatic embryogenesis and acclimatized in a greenhouse. The hybrids of 'Hamlin' sweet orange + 'Indian Red' pummelo and 'Hamlin' sweet orange + 'Singapura' pummelo were confirmed by leaf morphology, chromosome counting and molecular analysis. These hybrids have potential to be used directly as rootstocks aiming blight, citrus tristeza virus, and Phytophthora-induced disease tolerance, as well as for rootstocks improvement programs.

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Section: Hibridação Somática Entre Citrus Sinensis (L) Osbeck E C Gmentioning

confidence: 99%

Somatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) Osbeck

Calixto

Filho

Mendes

et al. 2004

Pesq. agropec. bras.

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“…entre Citrus e espécies/gêneros relacionados, sexualmente incompatíveis que possuam características de interesse agronômico (GROSSER, GMITTER JÚNIOR, CHANDLER, 2000;LATADO et al, 2002).…”

Section: Genitores Referênciasunclassified

Fusão de protoplastos visando a reconstrução da laranja azeda

Carvalho¹

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PROTOPLAST FUSION AIMING THE RECONSTRUCTION OF SOUR ORANGE The aim of this work was to apply the technique of chemical fusion of protoplasts, in order to develop interspecific somatic hybrids between mandarins (Citrus reticulata) and pummelos (Citrus grandis), in order to produce similar to sour orange (Citrus aurantium). The sources for protoplasts were embryogenic suspension cultures of 'Page' tangelo (C. reticulata x C. paradisi) and 'Murcott' tangor (C. reticulata x C. sinensis) and young leaves from seedlings of 'Lau Tau' and 'Ogami' pummelos (Citrus grandis). After the fusion, the protoplasts were cultivated in the absence of light, until the formation of microcolonies, and were then cultivated in double-phase EME medium, supplemented with 13.33 g/L of maltose for embryogenesis induction. The globular embryos thus formed were transferred to EME medium with 25 g/L of sucrose and, when in cotyledonal stage, were transferred to a culture medium supplemented with 1.5 g/L of malt extract. The shoots obtained were grafted in vitro onto 'Hamlin' and 'Valencia' sweet oranges. The regenerated plants were cultivated in a greenhouse, over commercial substrate. In this process, 17 plants were obtained. These plants presented phenotypic conformation different from the genitors, with leaves with reduced size, round apex, dark green coloration, rough leaf blade and absence of developed petiole. The analysis by flow cytometry confirmed the diploid character of the regenerated plants. RAPD molecular markers presented a similar band pattern between the regenerated specimen and the genitor 'Page' tangelo. The protocol used for isolation, hybridization and cultivation of protoplasts, as well as for the regeneration and acclimatization of the plants allowed the obtainment of 17 plants from the combination of 'Page' tangelo + 'Lau Tau' pummelo, with phenotypic conformation different from the genitors, two plants from the combination of 'Murcott' tangor + 'Ogami' pummelo and one plant from the combination of Murcote' tangor + 'Lau Tau' pummelo.

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“…No Centro de Energia Nuclear na Agricultura-USP, foram criados, também por fusão de protoplastos, híbridos de tangerina 'Cleópatra' com limão 'Cravo' ou laranja 'Caipira ' (LATADO et al, 2002).…”

Section: Introductionunclassified

Citrandarins e outros híbridos de trifoliata como porta-enxertos nanicantes para a laranjeira 'Valência' (Citrus sinensis L. Osbeck).

Blumer¹

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Laranjeiras 'Valência' enxertadas em citrandarins e outros híbridos de trifoliata foram plantadas em 1988, em Itirapina (SP), num Latossolo Vermelho-amarelo-textura arenosa e conduzidas sem irrigação. Os citrandarins Sunki x English (1.628), Cleópatra x Rubidoux (1.660) e Cleópatra x English (710), induziram as maiores produções de frutos nas cinco e nas treze colheitas. Os citranges 'Troyer' e 'Carrizo' foram significativamente menos produtivos que o Sunki x English (1.628) nas cinco primeiras e no total das treze colheitas. Os citrandarins Clementina x Trifoliata (1.615), Cleópatra x Swingle, seleções (715) e (1.614), Cleópatra x Rubidoux (1.600) e Cleópatra x Christian (712) induziram plantas nanicas. Sunki x English (1.628), 'Troyer' e 'Carrizo' proporcionaram as maiores plantas e lideraram a produção de frutos e de sólidos solúveis no período 2001-2003. Nenhuma planta mostrou sintomas de suscetibilidade à tristeza ou ao declínio. As plantas enxertadas no trangpur Cravo x Carrizo (717), mostraram sintoma de incompatibilidade na região de enxertia. "Seedlings" dos citrandarins Cleópatra x Swingle (1.587), Cleópatra x Trifoliata (1.574) e Cleópatra x Rubidoux (1.600) foram mais resistentes à gomose de Phytophthora parasitica que os demais porta-enxertos. vii CITRANDARINS AND OTHERS TRIFOLIATE HYBRIDS AS ROOTSTOCKS FOR 'VALENCIA' SWEET ORANGE (Citrus sinensis L. Osbeck) TREES. AUTHOR: SILVIA BLUMER ADVISER: Dr. JORGINO POMPEU JUNIOR SUMMARY 'Valência' sweet orange trees budded onto citrandarins and others trifoliate hybrids rootstocks were planted in 1988 on a sandy textured Oxisol in São Paulo state, Brazil, and managed without irrigation. Tristeza and blight diseases are endemic in the area. Trees on Sunki x English (1628), Cleopatra x Rubidoux (1660) and Cleopatra x English (710), produced the highest cumulative yields in the first five and in the thirteen crops. 'Carrizo' and 'Troyer' citranges gave the lowest productions in the first five yields but were similar to Sunki x English (1628) in 13-years cumulative yields. Clementine x Trifoliate (1615), Cleopatra x Swingle (715) and (1614) selections, Cleopatra x Rubidoux (1600) and Cleopatra x Christian (712) induced dwarfed trees. Sunki x English (1628) and 'Troyer' and 'Carrizo' citranges induced the largest trees and fruit and soluble solids production by tree in the 2001-2003 period. None tree showed symptoms of tristeza or declinio. Trees on Cravo x Carrizo (717) showed bud-union-ring symptom of incompatibility. Seedlings of Cleopatra x Swingle (1587), Cleopatra x Trifoliate (1574) and Cleopatra x Rubidoux (1600) were more resistent to Phytophthora parasitica infections than the others rootstocks.

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Obtenção de híbridos somáticos de limão 'Cravo' e tangerina 'Cleópatra'

Cited by 5 publications

References 15 publications

Somatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) Osbeck

Somatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) Osbeck

Fusão de protoplastos visando a reconstrução da laranja azeda

Citrandarins e outros híbridos de trifoliata como porta-enxertos nanicantes para a laranjeira 'Valência' (Citrus sinensis L. Osbeck).

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