Obtaining Wisent early blastocyst in vitro is a basic for protection and creation of biodiversity for this threatened species

Duszewska, A. M.; Gręda, Paweł; Baraniewicz, M.; Bielecki, Wojciech; Niżański, Wojciech; Partyka, Agnieszka; Tracz, Michał; Nowak, Zuzanna; Chełmońska-Soyta, Anna; Olech, Wanda

doi:10.1111/rda.13168

Cited by 7 publications

(5 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Wisent reproduction has been discussed in several articles [17][18][19][20][21][22][23][24][25]. However, there is a paucity of information focused on detailed characteristic of the male gamete in context of cryopreservation process in this species [26].…”

Section: Introductionmentioning

confidence: 99%

The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa

et al. 2022

View full text Add to dashboard Cite

Background The wisent (Bison bonasus) is a species that has undergone a population bottleneck. Homozygosity is prevalent within the population and may have a negative impact on semen quality in wisent bulls. Semen samples containing a large amount of functionally and morphologically impaired or dead spermatozoa have lower tolerance for cryopreservation process. Such samples are prone to involve damage acrosomes, to produce and release reactive oxygen which negatively affects proper function of spermatozoas. It is a good practice to select intact and viable gametes before subjecting the sample to cryopreservation to improve the efficiency of this process. The aim of this study was to assess the ability of Percoll® density gradient centrifugation in order to improve the quality of wisent spermatozoa after cryopreservation. Spermatozoa samples were analysed with computer-assisted semen analysis system and flow cytometry. Results Percoll® density gradient centrifugation resulted in increased percentage of motile spermatozoa, higher proportion of spermatozoa with normal morphology and proper functionality but also in a significant reduction of the total number of gametes. Nevertheless, the concentration of frozen spermatoza was still sufficient for obtaining a few complete insemination doses suggested for cattle from each epididymis. Conclusions While creating a high-quality genetic reserve, for in vitro fertilisation purposes, eliminating detritus and improving the overall quality of samples is more important than total number of spermatozoa. For these reasons, the achievement of higher post thaw quality of spermatozoa justifies the purification of samples by centrifugation in a Percoll® density gradient prior to the cryopreservation process.

show abstract

Section: Introductionmentioning

confidence: 99%

The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa

et al. 2022

View full text Add to dashboard Cite

show abstract

“…As a result of the cited study, domestic cattle and wisent hybrids were born [9]. With the use of gametes frozen in the Tris, egg yolk and glycerol based extender (TEG), an European bison early blastocyst was obtained in vitro [21]. The mentioned studies prove the fertilization capacity of European bison spermatozoa collected and cryopreserved in this way.…”

Section: Discussionmentioning

confidence: 99%

The first report on the use of a zona pellucida binding assay to compare the effects of European bison (Bison bonasus) epididymal spermatozoa cryopreservation in two different extenders

Eberhardt,

Colombo,

Prochowska

et al. 2023

Preprint

View full text Add to dashboard Cite

The wisent (or European bison) Bison bonasus is a species that has gone through a bottleneck, resulting in a narrow gene pool of the current population. Presently, the protection program for this species, in addition to protecting the habitat, is aimed at preserving gene pool. One of its elements is creating a bank of gametes with the intention of using them in constantly developed Assisted Reproductive Techniques in this species. In order to use the potential of the gametes stored in the bank as effectively as possible, it is extremely important to assess their post-freezing quality. Wisent epididymal spermatozoa stored in the bank are evaluated using basic semen analysis methods, the CASA system and flow cytometry. So far, functional tests of spermatozoa have not been introduced to the evaluation protocol of spermatozoa obtained post mortem from European bison. This article, for the first time describes the use of the zona pellucida binding test to assess the quality of cryopreserved wisent epididymal spermatozoa. Due to the close relationship between the species, the Zona binding Assay protocol developed for cattle was used. To exclude the influence of the composition of the extender used for cryopreservation, the spermatozoa were cryopreserved in the Extender based on Tris buffer, egg yolk and glycerol and in the commercial extender Andromed(Minitube GMBH). Regardless of the diluent used, 100% of the oocytes had sperm attached. The average number of spermatozoa attached to the oocyte was 66.26 for the Tris-based diluent group and 27.37 for the Andromed (Minitube GMBH) - frozen group. The advantage of the Tris based extender resulted from ZBA seems to coincide with the results of the basic and advanced semen evaluation. The conducted experiment showed that the ZBA protocol developed for cattle is suitable for the evaluation of wisent epididymal spermatozoa.

show abstract

“…Further research on wisent reproduction made it possible to obtain, as a result of classical in vitro fertilization, an early blastocyst from germ cells collected post mortem (16).…”

Section: Oocyte Harvesting In Vitro Fertilizationmentioning

confidence: 99%

Assisted reproductive techniques in wisents: Achievements and further challenges

Eberhardt

Niżański

Olech

et al. 2021

Medycyna Weterynaryjna

View full text Add to dashboard Cite

The current population of wisents (Bison bonasus, Linnaeus 1758) originates from only 12 individuals that survived World War II in zoos and private enclosures. For better preservation of genetic variability, research is being conducted to introduce assisted reproductive techniques in this species. In this article, we describe the current state of knowledge and prospects for further development in this field. The article is divided into sections dealing with semen collection and preservation, artificial insemination, oocyte collection, in vitro fertilization and embryo transfer, and further challenges.

show abstract

Obtaining Wisent early blastocyst in vitro is a basic for protection and creation of biodiversity for this threatened species

Cited by 7 publications

References 11 publications

The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa

The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa

The first report on the use of a zona pellucida binding assay to compare the effects of European bison (Bison bonasus) epididymal spermatozoa cryopreservation in two different extenders

Assisted reproductive techniques in wisents: Achievements and further challenges

Contact Info

Product

Resources

About