Observations on the growth of mouse hepatitis virus (MHV-3) in mouse macrophages

Mallucci, Livio

doi:10.1016/0042-6822(65)90248-5

Cited by 48 publications

(32 citation statements)

References 9 publications

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“…The ability of the cells of the RES to phagocytose virus particles is well documented (15). Some viruses will replicate in macrophage cultures, for example mouse hepatitis virus (16,17), while others are phagocytosed without subsequently replicating, for example vaccinia virus (18,19). It is not possible to say at this stage what happens to Riley virus or the cells at the time of infection and during replication.…”

Section: Discussionmentioning

confidence: 99%

Studies on the Mechanism of Action of Riley Virus

Evans

Salaman

1965

The Journal of Experimental Medicine

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A study was made of the replication of Riley virus in various tissue cultures. It was observed that Riley virus replicated in primary mouse embryo cultures for 8 to 12 days. As they aged primary cultures became less susceptible to Riley virus infection, and subcultured cells were not susceptible. Suspensions of virus, in the absence of cells, were inactivated at 36.5°C. Serial passage by transfer of supernatant fluids to fresh embryo cultures was not successful. Replication of the virus was more active in mouse embryo liver cultures than in the cultures of the embryo minus the livers. In cultures of mouse macrophages, the supernatants remained infective throughout the life of the cultures (21 days). The virus was passed 23 times through fresh macrophage cultures over a period of 80 days, during which the original inoculum (106.0ID50/ml) was theoretically diluted to 10–14. The possibility that the cells of the RES are involved in Riley virus replication is discussed.

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Section: Discussionmentioning

confidence: 99%

Studies on the Mechanism of Action of Riley Virus

Evans

Salaman

1965

The Journal of Experimental Medicine

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“…Cultured mouse peritoneal macrophages are highly susceptible to MHV-3 and the resulting in vitro infection evolves rapidly with formation of large syncytia which are characterized by extensive lysosomal damage (Allison & MaUucci, 1965;Mallucci, 1965Mallucci, , 1966. The mechanisms whereby cell fusion is induced in the course of a virus infection are not well understood, but structural alterations of the cell surface are of obvious importance (Poste & Pasternak, 1978;Knutton, 1980).…”

Section: Discussionmentioning

confidence: 99%

“…Sera were collected 4 weeks later, and pooled and assayed for neutralizing activity. Virus antigen was detected by indirect immunofluorescence using immune and control sera and fluorescein-labelled goat anti-mouse IgG preadsorbed on macrophage cultures (Mallucci, 1965). For acid phosphatase staining, an azo-dye-based method was used (Barka, 1960) and cells were counterstained with Mayer's haemalum nuclear stain.…”

Section: Discussionmentioning

confidence: 99%

Influence of the Cytoskeleton on the Expression of a Mouse Hepatitis Virus (MHV-3) in Peritoneal Macrophages: Acute and Persistent Infection

Mallucci

Edwards

1982

Journal of General Virology

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“…DISCUSSION The data presented in this paper indicate that the DBT cell culture provides a useful medium for plaque assay and propagation of MHV-2 strain of MHV. Propagation of MHV has been achieved in a variety of tissue cultures, such as explant cultures of various mouse tissues [1,2,21,22], primary cultures of mouse macrophages [13,20,24], mouse liver [15,18], kidney [16], spleen [14,24], embryonic cells [5,6,16], and cultures of continuous mouse cell lines [7,14,16]. Nevertheless, it has still remained difficult to obtain consistent results with this virus, and the present findings with DBT cells could be of considerable importance.…”

Section: Factors Influencing Plaque Formationmentioning

confidence: 99%

Mouse Hepatitis Virus (MHV‐2)

Hirano

Fujiwara

Matumoto

1976

Japanese Journal of Microbiology

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Various factor sinfluencing the plaque formation of mouse hepatitis virus (MHV‐2) in DBT cell monolayers were studied and a practical method for plaque assay was developed. Infected DBT cells yielded high‐titered virus and were a satisfactory source of complement‐fixing viral antigen. The predominant cytopathic effect of MHV‐2 in DBT cells was cell rounding and detachment, but no syncytial formation was observed. Fluorescent antibody staining revealed specific fluorescence only in the cytoplasm of infected DBT cells. In one‐step growth experiment, newly formed virus was first recognized within 4‐hr postinfection and showed subsequently a rapid exponential increase. Release of newly formed virus from the cell was rapid, and a continuous release lasted for a certain period of time. The average per‐cell yield of active virus was estimated to be about 6–7 × 102 plaque‐forming units.

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Observations on the growth of mouse hepatitis virus (MHV-3) in mouse macrophages

Cited by 48 publications

References 9 publications

Studies on the Mechanism of Action of Riley Virus

Studies on the Mechanism of Action of Riley Virus

Influence of the Cytoskeleton on the Expression of a Mouse Hepatitis Virus (MHV-3) in Peritoneal Macrophages: Acute and Persistent Infection

Mouse Hepatitis Virus (MHV‐2)

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