Oblique incidence reflectometry: optical models and measurements using a side-viewing gradient index lens-based endoscopic imaging system

Wall, R. Andrew; Barton, Jennifer K.

doi:10.1117/1.jbo.19.6.067002

Cited by 5 publications

(1 citation statement)

References 43 publications

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“…Indirect methods are more complicated in their use, involve mathematical models and/or simulations of light propagation, and require sophisticated (and sometimes expensive) instrumentation. [13][14][15][16] Time-resolved diffuse reflectance, 17,18 frequency domain, [19][20][21] spatially resolved continues wave, [22][23][24][25][26] low coherence interferometry, 27,28 and oblique incidence reflectometry 29,30 are among the methods used to extract optical properties of sample tissue. Each of these methods has varying capabilities regarding accuracy, levels of noise and artifacts, field of view (FOV), spatial and temporal resolution, speed, penetration depth, etc.…”

Section: Introductionmentioning

confidence: 99%

Multimodal optical setup based on spectrometer and cameras combination for biological tissue characterization with spatially modulated illumination

Baruch

Abookasis

2017

J. Biomed. Opt

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The application of optical techniques as tools for biomedical research has generated substantial interest for the ability of such methodologies to simultaneously measure biochemical and morphological parameters of tissue. Ongoing optimization of optical techniques may introduce such tools as alternative or complementary to conventional methodologies. The common approach shared by current optical techniques lies in the independent acquisition of tissue’s optical properties (i.e., absorption and reduced scattering coefficients) from reflected or transmitted light. Such optical parameters, in turn, provide detailed information regarding both the concentrations of clinically relevant chromophores and macroscopic structural variations in tissue. We couple a noncontact optical setup with a simple analysis algorithm to obtain absorption and scattering coefficients of biological samples under test. Technically, a portable picoprojector projects serial sinusoidal patterns at low and high spatial frequencies, while a spectrometer and two independent CCD cameras simultaneously acquire the reflected diffuse light through a single spectrometer and two separate CCD cameras having different bandpass filters at nonisosbestic and isosbestic wavelengths in front of each. This configuration fills the gaps in each other’s capabilities for acquiring optical properties of tissue at high spectral and spatial resolution. Experiments were performed on both tissue-mimicking phantoms as well as hands of healthy human volunteers to quantify their optical properties as proof of concept for the present technique. In a separate experiment, we derived the optical properties of the hand skin from the measured diffuse reflectance, based on a recently developed camera model. Additionally, oxygen saturation levels of tissue measured by the system were found to agree well with reference values. Taken together, the present results demonstrate the potential of this integrated setup for diagnostic and research applications.

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Section: Introductionmentioning

confidence: 99%

Multimodal optical setup based on spectrometer and cameras combination for biological tissue characterization with spatially modulated illumination

Baruch

Abookasis

2017

J. Biomed. Opt

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Energy flow difference structure design based on micro hemisphere structure

Fan¹,

Shi²,

Xia³

2019

Tenth International Conference on Thin Film Physics and Applications (TFPA 2019)

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Fiber-bundle microendoscopy with sub-diffuse reflectance spectroscopy and intensity mapping for multimodal optical biopsy of stratified epithelium

Greening

James

Powless

et al. 2015

Biomed. Opt. Express

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Early detection of structural or functional changes in dysplastic epithelia may be crucial for improving long-term patient care. Recent work has explored myriad non-invasive or minimally invasive "optical biopsy" techniques for diagnosing early dysplasia, such as high-resolution microendoscopy, a method to resolve sub-cellular features of apical epithelia, as well as broadband sub-diffuse reflectance spectroscopy, a method that evaluates bulk health of a small volume of tissue. We present a multimodal fiber-based microendoscopy technique that combines highresolution microendoscopy, broadband (450-750 nm) sub-diffuse reflectance spectroscopy (sDRS) at two discrete source-detector separations (374 and 730 μm), and sub-diffuse reflectance intensity mapping (sDRIM) using a 635 nm laser. Spatial resolution, magnification, field-of-view, and sampling frequency were determined. Additionally, the ability of the sDRS modality to extract optical properties over a range of depths is reported. Following this, proof-of-concept experiments were performed on tissuesimulating phantoms made with poly(dimethysiloxane) as a substrate material with cultured MDA-MB-468 cells. Then, all modalities were demonstrated on a human melanocytic nevus from a healthy volunteer and on resected colonic tissue from a murine model. Qualitative in vivo image data is correlated with reduced scattering and absorption coefficients. References and links 1. P. Sharma and E. Montgomery, "Gastrointestinal dysplasia," Pathology 45(3), 273-285 (2013). 2. P. M. Speight, "Update on oral epithelial dysplasia and progression to cancer," Head Neck Pathol. 1(1), 61-66 (2007). 3. Y. Zhang, "Epidemiology of esophageal cancer," World J. Gastroenterol. 19(34), 5598-5606 (2013). 4. N. Harpaz and A. D. Polydorides, "Colorectal dysplasia in chronic inflammatory bowel disease: pathology, clinical implications, and pathogenesis," Arch. Pathol. Lab. Med. 134(6), 876-895 (2010). 5. M. Ponz de Leon and C. Di Gregorio, "Pathology of colorectal cancer," Dig. Liver Dis. 33(4), 372-388 (2001). 6. M. J. Arends, C. H. Buckley, and M. Wells, "Aetiology, pathogenesis, and pathology of cervical neoplasia," J.Clin. Pathol. 51(2), 96-103 (1998 170-173 (2005). 11. M. Gu, H. Bao, and H. Kang, "Fibre-optical microendoscopy," J. Microsc. 254(1), 13-18 (2014) properties of pigmented skin lesions including melanoma using oblique incidence diffuse reflectance spectrometry," J.

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Oblique incidence reflectometry: optical models and measurements using a side-viewing gradient index lens-based endoscopic imaging system

Cited by 5 publications

References 43 publications

Multimodal optical setup based on spectrometer and cameras combination for biological tissue characterization with spatially modulated illumination

Multimodal optical setup based on spectrometer and cameras combination for biological tissue characterization with spatially modulated illumination

Energy flow difference structure design based on micro hemisphere structure

Fiber-bundle microendoscopy with sub-diffuse reflectance spectroscopy and intensity mapping for multimodal optical biopsy of stratified epithelium

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