2008
DOI: 10.1080/08905430802463487
View full text |Buy / Rent full text
|
Sign up to set email alerts
|

Abstract: One sorghum commercial genotype (MASSA 03) and nine ICRISAT high-lysine genotypes from India were analyzed for storage protein content, distribution profile, and soluble amino acid concentrations. Storage proteins fraction were extracted and separated by SDS-PAGE. Soluble amino acids contents were determined by HPLC. Variations in intensity and appearance and disappearance of protein bands were observed among the sorghum genotypes suggesting genetic variability. Amino acid profile also indicated large variatio… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

1
14
0

Year Published

2011
2011
2017
2017

Publication Types

Select...
2
1

Relationship

0
3

Authors

Journals

citations
Cited by 14 publications
(55 citation statements)
references
References 46 publications
(55 reference statements)
1
14
0
Order By: Relevance
“…The high lysine TG lines also showed considerable reduction in proline (24% lower), alanine (17%), glutamic acid/glutamine (15%), leucine (15%) and phenylalanine (15%), and increases in arginine (76% higher), aspartic acid (48%), methionine (42%) and glycine (32%) compared to the normal sorghums (Table 3). Similar changes in the amino acid profile of high-lysine cereals have been reported (Guiragossian et al, 1978;Singh and Axtell, 1973;Vendemiatti et al, 2008;Zarkadas et al, 2000). The altered amino acid profile observed for TG-P898012 (ABS032) and the backcrosses is probably a direct consequence of co-suppression of the synthesis of the major kafirin sub-classes.…”
Section: Protein Content and Amino Acid Profilesupporting
confidence: 52%
See 1 more Smart Citation
“…The high lysine TG lines also showed considerable reduction in proline (24% lower), alanine (17%), glutamic acid/glutamine (15%), leucine (15%) and phenylalanine (15%), and increases in arginine (76% higher), aspartic acid (48%), methionine (42%) and glycine (32%) compared to the normal sorghums (Table 3). Similar changes in the amino acid profile of high-lysine cereals have been reported (Guiragossian et al, 1978;Singh and Axtell, 1973;Vendemiatti et al, 2008;Zarkadas et al, 2000). The altered amino acid profile observed for TG-P898012 (ABS032) and the backcrosses is probably a direct consequence of co-suppression of the synthesis of the major kafirin sub-classes.…”
Section: Protein Content and Amino Acid Profilesupporting
confidence: 52%
“…Because the kafirins contain essentially no lysine, these results indicate a substantial increase in the proportion of non-kafirin storage proteins and non-storage proteins in the grain, which are higher in lysine content. In other high-lysine sorghum genotypes, the proportion of lysine rich non-kafirin storage proteins (albumins, globulins and glutelins) was considerably higher compared to normal sorghums (Guiragossian et al, 1978;Vendemiatti et al, 2008). While in high-lysine opaque-2 mutant maize genotypes, the increase in lysine content is attributed to elongation factor-1α (EF-1α), a lysine rich (11% lysine) non-zein protein (Habben et al, 1995).…”
Section: Protein Content and Amino Acid Profilementioning
confidence: 99%
“…The transgenic lines had high protein digestibility, improved amino acid score, and protein digestibility corrected amino acid score in contrast to untransformed sorghum lines. According to Vendemiatti et al (2008) a commercial sorghum line, Massa 03, and nine ICRISAT high lysine enriched sorghum lines from India, were evaluated for storage protein content and amino acid composition. The protein content and amino acid composition showed variation among the lines evaluated.…”
Section: Genetic Engineering/biofortificationmentioning
confidence: 99%
“…The prolamin fraction was less complex and more concentrated within the 23-26kDa size range, which contains the more abundant α-kafirin storage proteins. The alkali-soluble glutelin fraction exhibited some similarities to the prolamin fraction, likely due to incomplete extraction of the prolamin fraction, but contained a wider diversity of protein bands visible across a greater size range, which was also verified on 2D gels, and has been reported in previous studies (191,192). It is hypothesised that the glutelin proteins are more diverse because they play both a role in connecting the protein:starch matrix as well as providing a source of hydrolytic enzymes for the breakdown of starch and protein during germination (193).…”
Section: Sds Page and Lc-ms/ms Protein Profilingmentioning
confidence: 63%
“…The development of high-lysine waxy lines with improved amino availability is central to this effort (52,81,104,154,191). Increased expression of albumins and globulins leads to a higher content of essential amino acids.…”
Section: Improving the Nutritional Value Of Sorghummentioning
confidence: 99%