Nutritional conditions of the novel freshwater Coccomyxa AP01 for versatile fatty acids composition

Abstract

Aims: To analyse the biomass production and fatty acids (FAs) profiles in a newly isolated chlorophyte, namely Coccomyxa AP01, under nutritionally-balanced (NB) conditions (comparing nitrate and urea as nitrogen sources) and nitrogen or phosphate deprivation. Methods and Results:Lipid yields was about 30-40% of dried biomasses in all examined nutritional conditions. Under NB conditions lipids were principally constituted by monounsaturated FAs, mainly represented by oleic acid, and saturated and polyunsaturated FAs at similar concentrations. Nutrients deprivation induced remarkable changes in FAs profiles, with the highest amounts of saturated (42-46%), followed by similar amounts of monounsaturated and polyunsaturated, and the emergence of rare long-chain FAs. Under phosphate deprivation biomass yield was similar to NB conditions, with the highest yield of saturated (mainly palmitic acid) and of polyunsaturated FAs (33%) (mainly linoleic and linolenic acids).Conclusions: Balanced or deprived nutritional conditions in Coccomyxa AP01 induced a selective production and composition of FAs. The phosphate-deprivation condition concomitantly provided high biomass yield and the production of high value saturated and polyunsaturated FAs with industrial interest.

Orange Peel Waste as Feedstock for the Production of Glycerol-Free Biodiesel by the Microalgae Nannochloropsis oculata

“…The OWE50 medium facilitated N. oculata growth and oil production, with yields and growth parameters, i.e., specific growth rate, biomass yield, and productivity, higher than those obtained during the PA batch process. The FAs profiles of lipids produced by the same microalgal species under different culture conditions may vary significantly [15]. Similar results were obtained for N. oculata using the adopted nutritional regimens.…”

“…Several species of microalgae have been exploited as innovative sources in a variety of biotechnological applications to produce food, additive feed ingredients, cosmetics, biopharmaceuticals, nutraceuticals, pigments, and biofuels [13]. From a long-term sustainable energy perspective, microalgae are considered the most suitable raw material for thirdgeneration biofuel production [14] since their lipid fraction can be extracted and converted into biodiesel [15]. In comparison to oleaginous plants, which are used as a traditional source of oil for biodiesel production, microalgae have several advantages such as high growth rate, high lipid content, ability to survive in adverse conditions, no competition for exploitation of agricultural land and food production, and the ability to sequester CO 2 with higher efficiency [16].…”

Chemical Characterization, Antibacterial Activity, and Embryo Acute Toxicity of Rhus coriaria L. Genotype from Sicily (Italy)

“…The percentage of each FAMEs was calculated by comparison with the corresponding chromatogram peak area. The precision of the method was assessed in terms of RSD% analyzing five times each sample [ 19 ].…”

“…Approximately 0.50 g of R. coriaria sample was digested with 7 mL of HNO 3 (69% v / v ) and 1 mL of H 2 O 2 (30% v / v ) in a pre-washed PTFE vessel. The sample was then cooled down at room temperature, diluted up to 25 mL with ultrapure water, and filtered with a 0.45 µm filter [ 19 , 22 ]. The certified reference materials were processed using the same conditions.…”

Advances in Biosynthesis of Natural Products from Marine Microorganisms

“…There are some examples of high-performance thin-layer chromatography (HPTLC) being applied to extracts to screen, detect, and quantify their antioxidant and inhibitory activities against α-amylase [27], as well as acetylcholine esterase (AChE) inhibitory activity [29]. Recently HPTLC was applied in combination with microchemical (using reagents, such as DPPH free radical, p-anisaldehyde, and Fast Blue B) as well as biochemical (e.g., enzymatic activities of AChE and α-amylase) derivatizations to discover many compounds with bioactivities from marine algae extracts [30,33,34]. These compounds can serve as leads in developing novel therapeutics for the treatment or prevention of diabetes, neurodegenerative diseases, and cancer.…”