Nutritional composition of maize husk silage generated from solid state fermentation by Trichoderma viride UP01

Thana, Somchart; Tosawat, Anorach; Sorachakula, Choke; Danmek, Khanchai

doi:10.30848/pjb2019-6(28)

Cited by 3 publications

(2 citation statements)

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“…Maftukhah and Abdullah [22] also reported that A. niger can produce cellulase from rice straw under the SSF method with the optimum incubation time being 6 days at 30 °C. Trichoderma viride can produce cellulase using maize husk silage with the highest activity after 7 days of incubation at 30 °C through the SSF method [23]. The difference in incubation time is due to differences in substrates, types of microorganisms, and other factors in the fermentation process.…”

Section: Cellulase Productionmentioning

confidence: 99%

Cellulase Production from Paecilomyces Lilacinus ICP1 Using Coffee Pulp as Substrate

Trianawati¹,

Winarsa²,

Siswoyo³

et al. 2022

Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)

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Cellulase can be produced by a microorganism, such as fungi. The cellulolytic microorganism used in this study was Paecilomyces lilacinus ICP1. P. lilacinus ICP1 is one of the indigenous fungi from coffee pulp that has been known to produce cellulose. Coffee pulp is expected to be able to replace the use of pure cellulose substrate because of its high cellulose content of 63% and other organic materials that can support the growth of cellulolytic microorganisms. One method that is often used for enzyme production by utilizing agricultural or plantation waste is Solid State Fermentation (SSF). Cellulase production by Paecilomyces lilacinus ICP1 using coffee pulp waste as a substrate has been investigated. Through Solid State Fermentation of coffee pulp substrate based the P. lilacinus ICP1 can produce cellulase up to 0.17 U/ml with 3 days of incubation at 30 °C. Cellulase activity was measured base on reducing sugar produced using the Somogyi-Nelson method against 0.5% carboxymethyl cellulose in 20 mM acetate buffer pH 5. The crude cellulase was then dialyzed through a cellulose membrane with 12-14 kDa pores in the same buffer. In this step, the activity was 0.47 U/ml, an increase about three times from the initial. These results proved that P. lilacinus ICP1 could secrete extracellular cellulase through Solid State Fermentation using coffee pulp waste without any nutrients added so that it may become a strategy industrial scale to produce cellulase cheaply.

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Section: Cellulase Productionmentioning

confidence: 99%

Cellulase Production from Paecilomyces Lilacinus ICP1 Using Coffee Pulp as Substrate

Trianawati¹,

Winarsa²,

Siswoyo³

et al. 2022

Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)

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show abstract

“…Also, Sharma and Arora (2011) reported the use of fungal inoculums for the fermentation of agricultural residues in order to improve the nutrition values of substrates. During solid-state fermentation (SSF), the increased nutrition values were due to bioconversion of substrate that had been degraded by fungal enzymes (Thana et al 2019). Maize cob contains 5.22% protein, anti-nutrients, such as saponin (2.09%), tannin (0.03%) and phytate (0.72%) (Ukanwoko and Nwachukwu 2017), cellulose (27.71%) and hemicellulose (38.78%) but also contain a significant amount of lignin (9.4%) (Shinners et al 2007), calcium (17.19 mg/100 g), potassium (375.25 mg/100 g), sodium (129.19 mg/100 g) (Abubakar et al 2016) and antioxidant activity of 0.3-10 µmol/ gdw (Nawaz et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Blood chemistry of West African dwarf goats fed treated maize cob- and maize husk-based diets with mixture of microorganisms

2021

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Background Twenty-four West African Dwarf (WAD) goats with average weight of 6.57 ± 0.56 kg were used in investigating the effect of microbial-treated maize cob and husk diets on blood profile. The goats were assigned to six dietary treatments replicated four times in a completely randomized design. Chopped maize cobs and husks were sterilized for 60 min to eliminate any form of contamination and then inoculated with mixture of 15 ml each of Lactobacillus delbrueckii and Neurospora crassa and incubated anaerobically for 5 days and then air-dried. The treated maize cob and husk were incorporated in the diets at the rate of 0% (diet), 20% (diet B), 30% (diet C) [maize cob diets], and 0% (diet D), 20% (diet E), 30% (diet F) [maize husk diets], respectively. The animals were acclimatized for two weeks while the experimental period lasted for 56 days. A completely randomized design was adopted. Results The results showed that the dietary treatments significantly (P < 0.05) influenced the erythrocyte sedimentation rate which ranged from 0.50 to 1.00 mm/hr. Significant (P < 0.05) differences were observed in the globulin (28.16 g/dl diet A—59.26 g/dl diet B), aspartate aminotransferase (48.00 u/l diet E—128. 25 u/l diet C) and alanine aminotransferase (8.20 u/l diet A—33.33 u/l diet D). Serum electrolytes values were significantly (P < 0.05) influenced by dietary treatments. Conclusion Based on the findings of this study, microbial-treated maize cob and husk diets supported erythropoiesis did not induce any liver damage and the serum electrolytes of the animals were improved. Hence, microbial-treated maize cob and husk can be a suitable alternative feed source at 30% incorporation in a complete diet for WAD goats as adverse implications on the health of the animals was not observed.

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Occurrence of an invertase producing strain of Aspergillus niger LP5 isolated from longan pollen and its application in longan syrup production to feed honey bees (Apis mellifera L.)

et al. 2022

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Nutritional composition of maize husk silage generated from solid state fermentation by Trichoderma viride UP01

Cited by 3 publications

References 0 publications

Cellulase Production from Paecilomyces Lilacinus ICP1 Using Coffee Pulp as Substrate

Cellulase Production from Paecilomyces Lilacinus ICP1 Using Coffee Pulp as Substrate

Blood chemistry of West African dwarf goats fed treated maize cob- and maize husk-based diets with mixture of microorganisms

Occurrence of an invertase producing strain of Aspergillus niger LP5 isolated from longan pollen and its application in longan syrup production to feed honey bees (Apis mellifera L.)

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