Nutrient concentrations in murine follicular fluid and the female reproductive tract

Harris, Sarah E.; Gopichandran, Nadia; Picton, Helen M.; Leese, Henry J.; Orsi, Nicolas M.

doi:10.1016/j.theriogenology.2005.01.004

Cited by 199 publications

(141 citation statements)

References 72 publications

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“…Mouse oviductal fluid at estrus has been found to contain phenylalanine (202 mM) and tryptophan (50 mM) concentrations that are adequate to drive LAAO activity (Harris et al 2005). Human follicular fluid also contains significant amounts of these amino acids (w70 and 30 mM respectively) that, in the long term, might trigger Acrosomal exocytosis was also significantly increased by the presence of phenylalanine in absence and presence of progesterone; however in the presence of the latter, the impact of phenylalanine was evident at lower doses (2.5 mM).…”

Section: Discussionmentioning

confidence: 99%

Human spermatozoa possess an IL4I1 l-amino acid oxidase with a potential role in sperm function

Houston¹,

Curry²,

Aitken³

2015

Reproduction

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Reactive oxygen species (ROS) are known to play an important role in the regulation of human sperm function. In this study, we demonstrate for the first time that human spermatozoa possess interleukin-induced gene 1 (IL4I1), an L-amino acid oxidase (LAAO) which is capable of generating ROS on exposure to aromatic amino acids in the presence of oxygen. The preferred substrates were found to be phenylalanine and tryptophan while the enzyme was located in the acrosomal region and midpiece of these cells. In contrast to equine and bovine spermatozoa, enzyme activity was lost as soon as the spermatozoa became non-viable. On a cell-to-cell basis human spermatozoa were also shown to generate lower levels of hydrogen peroxide than their equine counterparts on exposure to phenylalanine. Stimulation of LAAO activity resulted in the induction of several hallmarks of capacitation including tyrosine phosphorylation of the sperm flagellum and concomitant activation of phospho-SRC expression. In addition, stimulation of LAAO resulted in an increase in the levels of acrosomal exocytosis in both the presence and absence of progesterone stimulation, via mechanisms that could be significantly reversed by the presence of catalase. As is often the case with free radical-mediated phenomena, prolonged exposure of human spermatozoa to phenylalanine resulted in the stimulation of apoptosis as indicated by significant increases in mitochondrial superoxide generation and the activation of intracellular caspases. These results confirm the existence of an LAAO in human spermatozoa with a potential role in driving the redox regulation of sperm capacitation and acrosomal exocytosis.

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Section: Discussionmentioning

confidence: 99%

Human spermatozoa possess an IL4I1 l-amino acid oxidase with a potential role in sperm function

Houston¹,

Curry²,

Aitken³

2015

Reproduction

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“…In dairy heifers, an association was observed between the total concentration of free amino acids in the plasma and oocyte cleavage, suggesting that dietary amino acids could mediate the oocyte quality (Rooke et al, 2009);however, Sinclair, et al (2000) also showed that in heifers excessive levels of protein in the diet negatively affected the subsequent development of oocytes in vitro (Sinclair et al, 2000) In comparison with carbohydrate metabolism, and despite their important roles, there is little information about aminoacid uptake and metabolism by follicles and oocytes, especially during the earlier stages of folliculogenesis in cattle. Studies measuring amino acids in follicular fluid and the reproductive tract in mice have provided valuable information on the availability of substrates, reporting significantly higher levels of some amino acids in the follicular fluid compared with the reproductive tract (Harris et al, 2005). In cattle, LH has been shown to increase glutamine oxidative metabolism by oocytes and COCs (Zuelke and Brackett, 1993).…”

Section: Effect Of Amino Acidsmentioning

confidence: 99%

The effect of nutrition and metabolic status on the development of follicles, oocytes and embryos in ruminants

Dupont

Scaramuzzi

Reverchon

2014

animal

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The impact of nutrition and energy reserves on the fertility of ruminants has been extensively described. However, the metabolic factors and the molecular mechanisms involved in the interactions between nutrition and ovarian function are still poorly understood. These factors could be hormonal (either reproductive and/or metabolic) and/or dietary and metabolic (glucose, amino acids and fatty acids). In this review, we briefly summarize the impact of those nutrients (fatty acids, glucose and amino acids) and metabolic hormones (insulin/IGF-I, growth hormone, T3/4, ghrelin, apelin and the adipokines (leptin, adiponectin and resistin)) implicated in the development of ovarian follicles, oocytes and embryos in ruminants. We then discuss the current hypotheses on the mechanisms of action of these factors on ovarian function. We particularly describe the role of some energy sensors including adenosine monophosphate-activated kinase and peroxisome proliferator-activated receptors in the ovarian cells.

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“…One-cell mouse embryos will develop through the 2-cell block in media that are up to about 70 mOsM more hypertonic when glycine is present than when it is absent (Hadi et al, 2005). Glycine is also abundant in the embryo's environment in vivo, with 0.5-3.0 mM glycine in oviductal fluid (Guerin et al, 1995;Harris et al, 2005), far above the half-maximally effective concentration of 50 mM for protecting embryo development against increased osmolarity (Dawson and Baltz, 1997). Additionally, a single, robust glycine transport mechanism was shown to be present and active in early preimplantation embryos until compaction, and had been identified as the classical amino acid transport system designated GLY (Van Winkle et al, 1988).…”

Section: Organic Osmolyte Usage By Preimplantation Embryosmentioning

confidence: 99%

Cell volume regulation in mammalian oocytes and preimplantation embryos

Baltz

Zhou

2012

Molecular Reproduction Devel

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SUMMARYThe earliest stages of preimplantation embryos are particularly sensitive to increased osmolarity, even within the physiological range. This sensitivity contributed to persistent developmental arrest, even when embryos were cultured in vitro in older, conditioned culture media, and seems to arise when embryos at the 1-and 2-cell stages accumulate inorganic ions used for cell volume homeostasis at too high a level, through activation of coupled Na þ /H þ and HCO 3 À /Cl À exchange. Such accumulation of inorganic ions can be disruptive since, above a certain level, the increased ionic strength disrupts cellular biochemistry and macromolecular functions and alters membrane potential. To counter this, embryos have evolved mechanisms of cell volume regulation that are unique to early preimplantation embryogenesis. The primary role of these is glycine accumulation via the GLYT1 transporter, with a secondary contribution by betaine accumulation via the SIT1 transporter. Independent cell-volume regulation first arises in the oocyte only after ovulation is triggered, when the strong oocyte-zona pellucida adhesion present in germinal vesicle stage oocytes in the ovarian follicle is released and GLYT1 becomes activated to begin accumulating glycine. Open questions still remain regarding how these processes are regulated.Mol. Reprod. Dev. 79: 821-831, 2012. ß

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Nutrient concentrations in murine follicular fluid and the female reproductive tract

Cited by 199 publications

References 72 publications

Human spermatozoa possess an IL4I1 l-amino acid oxidase with a potential role in sperm function

Human spermatozoa possess an IL4I1 l-amino acid oxidase with a potential role in sperm function

The effect of nutrition and metabolic status on the development of follicles, oocytes and embryos in ruminants

Cell volume regulation in mammalian oocytes and preimplantation embryos

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