Numerical simulation of double emulsion formation in cross-junctional flow-focusing microfluidic device using Lattice Boltzmann method

Askari, Amir Hossein; Shams, Mehrzad; Sullivan, Pierre E.

doi:10.1080/01932691.2018.1518141

Cited by 12 publications

(5 citation statements)

References 49 publications

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“…In this regard, higher IA:LO ratios lead to a dripping regime, while lower ones produce a pseudo-jetting regime where elongated droplets with weaker surface tension are formed. For all the evaluated FRRs (see Supplementary Materials, Table S3) a dripping regime is largely predominant, which is likely to translate into forming monodisperse droplets [85]. In addition, droplet formation within the device was verified by direct microscopic visualization, which indicates that the generated pressures are enough to produce inner and middle phases break up into these structures, as predicted by the in silico analyses (See Supplementary Materials, Video S1, Video S2, Video S3).…”

Section: Low-cost Octanol-assisted Liposomes Assemblymentioning

confidence: 60%

“…This can be explained from the dimensions of the channels of the manufactured device, which are crucial to maintain a laminar regime within the microsystem and to have finer control over the size of the formed emulsions [61]. The uniformity of the synthesized GUVs was under 0.4 for all evaluated operating conditions, indicating that monodispersity was preserved to an acceptable degree as expected for twostep double emulsion generators [85]. Finally, there is no apparent relationship between the LO phase concentration and the GUVs size.…”

Section: Guvs Characterizationmentioning

confidence: 85%

“…This is closely linked to the inner flow rate and the IA:LO ratio, whereby by increasing the FRR an increase in the difference between center and side-channel inflows is observed. This generates smaller and more compact simple emulsions, leading to GUVs of lower size [61,85]. This relationship is no longer applicable at higher concentrations (0.02% and 0.04%) as evidenced by the increase in size obtained for higher FRR and the results obtained in the statistical comparisons.…”

Section: Guvs Characterizationmentioning

confidence: 96%

“…According to the digital microscopy images presented in Figure 4e,f, the single emulsion is formed at the first flow-focusing unit while the double one is formed in the second flow-focusing unit after splitting the single emulsion into two droplets. The double emulsion was successfully formed in a two-step process where two dripping pressurebased instabilities take place in each flow-focusing unit, producing an inner aqueous phase droplet contained within a lipid-carrying organic phase outer droplet [84,85]. Depending on the FRR, different IA:LO ratios might be achieved, which have a significant impact on defining the droplet generation regime.…”

Section: Low-cost Octanol-assisted Liposomes Assemblymentioning

confidence: 99%

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Design and Manufacture of a Low-Cost Microfluidic System for the Synthesis of Giant Liposomes for the Encapsulation of Yeast Homologues: Applications in the Screening of Membrane-Active Peptide Libraries

et al. 2021

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The discovery of new membrane-active peptides (MAPs) is an area of considerable interest in modern biotechnology considering their ample applicability in several fields ranging from the development of novel delivery vehicles (via cell-penetrating peptides) to responding to the latent threat of antibiotic resistance (via antimicrobial peptides). Different strategies have been devised for such discovery process, however, most of them involve costly, tedious, and low-efficiency methods. We have recently proposed an alternative route based on constructing a non-rationally designed library recombinantly expressed on the yeasts’ surfaces. However, a major challenge is to conduct a robust and high-throughput screening of possible candidates with membrane activity. Here, we addressed this issue by putting forward low-cost microfluidic platforms for both the synthesis of Giant Unilamellar Vesicles (GUVs) as mimicking entities of cell membranes and for providing intimate contact between GUVs and homologues of yeasts expressing MAPs. The homologues were chitosan microparticles functionalized with the membrane translocating peptide Buforin II, while intimate contact was through passive micromixers with different channel geometries. Both microfluidic platforms were evaluated both in silico (via Multiphysics simulations) and in vitro with a high agreement between the two approaches. Large and stable GUVs (5–100 µm) were synthesized effectively, and the mixing processes were comprehensively studied leading to finding the best operating parameters. A serpentine micromixer equipped with circular features showed the highest average encapsulation efficiencies, which was explained by the unique mixing patterns achieved within the device. The microfluidic devices developed here demonstrate high potential as platforms for the discovery of novel MAPs as well as for other applications in the biomedical field such as the encapsulation and controlled delivery of bioactive compounds.

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Section: Low-cost Octanol-assisted Liposomes Assemblymentioning

confidence: 60%

Section: Guvs Characterizationmentioning

confidence: 85%

Section: Guvs Characterizationmentioning

confidence: 96%

Section: Low-cost Octanol-assisted Liposomes Assemblymentioning

confidence: 99%

See 2 more Smart Citations

Design and Manufacture of a Low-Cost Microfluidic System for the Synthesis of Giant Liposomes for the Encapsulation of Yeast Homologues: Applications in the Screening of Membrane-Active Peptide Libraries

et al. 2021

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“…130 It has been widely applied in multicomponent multiphase flows, non-Newtonian fluid, fluid-solid coupling, interfacial dynamics, and porous media seepage, 131 and it is known as one of the most promising numerical simulation methods. 132 At present, the lattice Boltzmann method has been used to investigate the formation mechanism of emulsion droplets, 133 immiscible displacements in complex microchannels, 134 droplet relaxation and coalescence characteristics, 135 and deformation and fracture characteristics of emulsion droplets. 136 The applications of the lattice Boltzmann method in multiphase fluid and complex fluid flow are shown in Table structure of the actual core in seepage simulations, and it is more significant to study the microscopic seepage characteristics by the Boltzmann method in porous media.…”

Section: Numerical Simulation Of Microscopic Seepage Flowmentioning

confidence: 99%

A review of crude oil emulsification and multiphase flows in chemical flooding

Yazhou

Yin

et al. 2022

Energy Science & Engineering

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Chemical flooding has become an important method to enhance oil recovery in high‐water cut reservoirs. Crude oil emulsification often occurs during chemical flooding, and it plays a very positive role in increasing crude oil production. Crude oil emulsification increases the complexity of fluid flow in the reservoir including multifield coupling characteristics and multiphase flow and multiphase form characteristics. This paper discusses advanced research techniques and the status of multiphase fluid flows in chemical flooding, including the interfacial rheological properties of emulsions, physical simulation of emulsion seepage, and mathematical models and numerical simulations of seepage. Studies on the mechanism of seepage have analyzed the macroscopic and microscopic aspects of seepage during chemical flooding. Prospective directions for future research are indicated including the study of the interfacial rheological characteristics of emulsions, methods for the evaluation of the seepage characteristics of chemical flooding, and mathematical models of multiphase seepage during chemical flooding.

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